Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Biological activity of crude polyphenol fractions of Cedrela sinensis isolated using different extraction methods

참죽의 추출방법에 따른 폴리페놀 분획의 생리기능성

  • Received : 2017.03.14
  • Accepted : 2017.05.09
  • Published : 2017.08.31
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Abstract

The biological activity of crude polyphenol fractions (WphF, EphE, VphF, and SphF) extracted from Cedrela sinensis using hot water, ethanol, and enzymes such as Viscozyme and Shearzyme was examined in this study. The yield of VphF was the highest (43.44%) among all fractions. The total polyphenol and flavonoid content of the fractions were highest after ethanol extraction (447.98 and 337.49 mg/g, respectively). Fractions obtained after hot water and ethanol treatment showed high antioxidant activity. All fractions, except for WphF, showed a significantly higher ${\alpha}$-glucosidase inhibitory activity than the acarbose. EphF and WphF showed the high acetylcholinesterase inhibition activity. All fractions showed more than 50% tyrosinase inhibition activity at 2 mg/mL concentration. According to these results, the crude polyphenol fractions from C. sinensis showed high antioxidative, ${\alpha}$-glucosidase inhibitory, and tyrosinase inhibitory activities. This study suggests that crude polyphenol fractions from C. sinensis, especially the WphF and EphF fractions, are good sources of functional food.

다양한 영양성분을 함유할 뿐만 아니라 생리활성이 우수한 참죽의 기능성 식품소재로의 이용가능성을 확인하고자 참죽으로부터 폴리페놀을 추출하고 이들의 생리기능성을 측정하였다. 이를 위해 동결건조된 참죽분말을 열수, 에탄올 및 효소(Viscozyme, Shearzyme)를 이용하여 폴리페놀을 추출하고 추출방법에 따른 폴리페놀 분획의 기능적 특성을 비교하였다. 참죽으로부터 추출된 폴리페놀 분획의 수율은 VphF가 43.44%로 가장 높았으며, 그 다음 SphF, WphF, EphF 순으로 나타났다. 총 폴리페놀 및 총 플라보노이드 함량은 EphF가 각각 447.98 mg GAE/g 및 337.49 mg QE/g으로 가장 높은 함량을 보였다. 산화방지 활성을 측정한 결과, DPPH 라디칼 소거능, ABTS 라디칼 소거능, $Fe^{2+}$ 킬레이팅 효과 및 환원력 모두 EphF가 유의적으로 가장 높았다. Acetylcholinesterase 저해활성을 측정한 결과, 저농도에서는 EphF가, 고농도에서는 WphF가 가장 높은 활성을 보였다. ${\alpha}$-glucosidase 저해활성은 EphF, VphF, SphF, WphF 순으로 높은 활성을 보였으며, 특히 EphF, SphF, VphF는 대조군인 아카보스보다 유의적으로 높은 활성을 보였다. Tyrosinase 저해활성 측정 결과, 2 mg/mL 농도에서 모든 추출군은 50% 이상의 저해활성을 보였다. 이상의 연구 결과, 참죽의 폴리페놀 분획은 우수한 산화방지 활성뿐만 아니라 ${\alpha}$-glucosidase, acetylcholinesterase, tyrosinase 효소에 대한 높은 저해활성을 보였으며, 특히 에탄올추출 및 열수추출에 의해 얻어진 폴리페놀 분획은 뛰어난 산화방지 활성을 보였다. 이를 통해 참죽은 기능성 식품 소재로 활용가능성이 매우 높을 것으로 판단된다.

Keywords

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