Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Stability of Retroviral Vectors Against Ultracentrifugation Is Determined by the Viral Internal Core and Envelope Proteins Used for Pseudotyping

  • Kim, Soo-hyun (Department of Chemical and Biological Engineering, Sookmyung Women's University) ;
  • Lim, Kwang-il (Department of Chemical and Biological Engineering, Sookmyung Women's University)
  • Received : 2017.03.21
  • Accepted : 2017.04.18
  • Published : 2017.05.31
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Abstract

Retroviral and lentiviral vectors are mostly pseudotyped and often purified and concentrated via ultracentrifugation. In this study, we quantified and compared the stabilities of retroviral [murine leukemia virus (MLV)-based] and lentiviral [human immunodeficiency virus (HIV)-1-based] vectors pseudotyped with relatively mechanically stable envelope proteins, vesicular stomatitis virus glycoproteins (VSVGs), and the influenza virus WSN strain envelope proteins against ultracentrifugation. Lentiviral genomic and functional particles were more stable than the corresponding retroviral particles against ultracentrifugation when pseudotyped with VSVGs. However, both retroviral and lentiviral particles were unstable when pseudotyped with the influenza virus WSN strain envelope proteins. Therefore, the stabilities of pseudotyped retroviral and lentiviral vectors against ultracentrifugation process are a function of not only the type of envelope proteins, but also the type of viral internal core (MLV or HIV-1 core). In addition, the fraction of functional viral particles among genomic viral particles greatly varied at times during packaging, depending on the type of envelope proteins used for pseudotyping and the viral internal core.

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References

  1. Akpinar, F., and Yin, J. (2015). Characterization of vesicular stomatitis virus populations by tunable resistive pulse sensing. J. Virol. Methods 218, 71-76. https://doi.org/10.1016/j.jviromet.2015.02.006
  2. Beer, C., Meyer, A., Muller, K., and Wirth, M. (2003). The temperature stability of mouse retroviruses depends on the cholesterol levels of viral lipid shell and cellular plasma membrane. Virology 308, 137-146. https://doi.org/10.1016/S0042-6822(02)00087-9
  3. Chai, N., Chang, H.E., Nicolas, E., Gudima, S., Chang, J., and Taylor, J. (2007). Assembly of hepatitis B virus envelope proteins onto a lentivirus pseudotype that infects primary human hepatocytes. J. Virol. 81, 10897-10904. https://doi.org/10.1128/JVI.00959-07
  4. Cronin, J., Zhang, X.Y., and Reiser, J. (2005). Altering the tropism of lentiviral vectors through pseudotyping. Curr. Gene Ther. 5, 387-398. https://doi.org/10.2174/1566523054546224
  5. Galipeau, J., Li, H., Paquin, A., Sicilia, F., Karpati, G., and Nalbantoglu, J. (1999). Vesicular stomatitis virus G pseudotyped retrovector mediates effective in vivo suicide gene delivery in experimental brain cancer. Cancer Res. 59, 2384-2394.
  6. Gruter, O., Kostic, C., Crippa, S.V., Perez, M.T., Zografos, L., Schorderet, D.F., Munier, F.L., and Arsenijevic, Y. (2005). Lentiviral vector-mediated gene transfer in adult mouse photoreceptors is impaired by the presence of a physical barrier. Gene Ther. 12, 942-947. https://doi.org/10.1038/sj.gt.3302485
  7. Hossain, A., Ali, K., and Shin, C.G. (2014). Nuclear localization signals in prototype foamy viral integrase for successive infection and replication in dividing cells. Mol. Cells 37, 140-148. https://doi.org/10.14348/molcells.2014.2331
  8. Jang, Y.H., Song, H.I., Yang, Y., and Lim, K.I. (2016). Reliable RTqPCR-based titration of retroviral and lentiviral vectors via quantification of residual vector plasmid DNA in samples. Biotechnol. Lett. 38, 1285-1291. https://doi.org/10.1007/s10529-016-2110-7
  9. Lim, K.I., Lang, T., Lam, V., and Yin, J. (2006). Model-based design of growth-attenuated viruses. PLoS Comput. Biol. 2, e116. https://doi.org/10.1371/journal.pcbi.0020116
  10. Lim, K.I., Klimczak, R., Yu, J.H., and Schaffer, D.V. (2010). Specific insertions of zinc finger domains into Gag-Pol yield engineered retroviral vectors with selective integration properties. Proc. Natl. Acad. Sci. USA 107, 12475-12480. https://doi.org/10.1073/pnas.1001402107
  11. Lim, J.Y., Nam, J.S., Yang, S.E., Shin, H., Jang, Y.H., Bae, G.U., Kang, T., Lim, K.I., and Choi, Y. (2015). Identification of newly emerging influenza viruses by surface-enhanced raman spectroscopy. Anal. Chem. 87, 11652-11659. https://doi.org/10.1021/acs.analchem.5b02661
  12. Lin, A.H., and Cannon, P.M. (2002). Use of pseudotyped retroviral vectors to analyze the receptor-binding pocket of hemagglutinin from a pathogenic avian influenza A virus (H7 subtype). Virus Res. 83, 43-56. https://doi.org/10.1016/S0168-1702(01)00407-5
  13. Naldini, L., Blomer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F.H., Verma, I.M., and Trono, D. (1996). In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263-267. https://doi.org/10.1126/science.272.5259.263
  14. Patel, M., Giddings, A.M., Sechelski, J., and Olsen, J.C. (2013). High efficiency gene transfer to airways of mice using influenza hemagglutinin pseudotyped lentiviral vectors. J. Gene Med. 15, 51-62. https://doi.org/10.1002/jgm.2695
  15. Peng, K.W., TenEyck, C.J., Galanis, E., Kalli, K.R., Hartmann, L.C., and Russell, S.J. (2002). Intraperitoneal therapy of ovarian cancer using an engineered measles virus. Cancer Res. 62, 4656-4662.
  16. Sanders, B.P., de Los Rios Oakes, I., van Hoek, V., Bockstal, V., Kamphuis, T., Uil, T.G., Song, Y., Cooper, G., Crawt, L.E., Martin, J., et al. (2016). Cold-adapted viral attenuation (CAVA): highly temperature sensitive polioviruses as novel vaccine strains for a next generation inactivated poliovirus vaccine. PLoS Pathog. 12, e1005483. https://doi.org/10.1371/journal.ppat.1005483
  17. Schaffer, D.V., Koerber, J.T., and Lim, K.I. (2008). Molecular engineering of viral gene delivery vehicles. Annu. Rev. Biomed. Eng. 10, 169-194. https://doi.org/10.1146/annurev.bioeng.10.061807.160514
  18. Segura, M.M., Kamen, A., and Garnier, A. (2006). Downstream processing of oncoretroviral and lentiviral gene therapy vectors. Biotechnol. Adv. 24, 321-337. https://doi.org/10.1016/j.biotechadv.2005.12.001
  19. Tao, L., Chen, J., Meng, J., Chen, Y., Li, H., Liu, Y., Zheng, Z., and Wang, H. (2013). Enhanced protective efficacy of H5 subtype influenza vaccine with modification of the multibasic cleavage site of hemagglutinin in retroviral pseudotypes. Virol. Sin. 28, 136-145. https://doi.org/10.1007/s12250-013-3326-5
  20. Vu, H.N., Ramsey, J.D., and Pack, D.W. (2008). Engineering of a stable retroviral gene delivery vector by directed evolution. Mol. Ther. 16, 308-314. https://doi.org/10.1038/sj.mt.6300350

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