대한본초학회지 (The Korea Journal of Herbology)

  • 제32권4호
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  • Pages.1-8
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  • 2017
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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누에 복합 추출물의 면역 활성 증진 효과

Evaluation of Immunopotentiation Activities of Combined Extract of Silkworm and Food material

  • 이아름 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김수현 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김수지 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김경조 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이영철 (상지대학교 한의과대학 본초학교실) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Lee, AhReum (College of Korean Medicine, Daegu Haany University) ;
  • Kim, SooHyun (College of Korean Medicine, Daegu Haany University) ;
  • Kim, SuJi (College of Korean Medicine, Daegu Haany University) ;
  • Kim, KyeongJo (College of Korean Medicine, Daegu Haany University) ;
  • Lee, Young-Cheol (College of Korean Medicine, Sangji University) ;
  • Roh, Seong-Soo (College of Korean Medicine, Daegu Haany University)
  • 투고 : 2017.06.11
  • 심사 : 2017.07.15
  • 발행 : 2017.07.30
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초록

Objectives : Silkworm is known as immunomodulatory substances and contain various bioactive compounds such as serine, tyrosine and alanine. The aim of this study was to investigated the immunopotentiating activity of combine extract that silkworm and food materials (Eucommia ulmoides, Angelica gigas, Acanthopanax, Allium hookeri, Cinnamomum cassia, Liriope platyphylla, Curcuma longa, Achyranthes japonica, Alpinia oxyphylla, Adenophora triphylla). Methods : Among 10 kinds of food materials, to select food materials with the effect of enhancing the immune function mouse splenocyte proliferation ability was measured by 3-[4,5-dimethylthiazol-2-y]-2,5-diphenyl terazolium bromide (MTT) assay. Then, combine extract of silkworm and food materials were evaluated that mouse splenocyte proliferation ability by EZ-cytox cell viability assay. Morever, cytokines production such as IL-2, IL-4, IL10, IL12, $IFN-{\gamma}$ on mouse T lymphocyte stimulated with concanavalin A (ConA) was measured. Results : Eucommia ulmoides, Acanthopanax, Allium hookeri, Cinnamomum cassia, Liriope platyphylla has high proliferation ability of mouse splenocyte compared with Curcuma longa, Achyranthes japonica, Alpinia oxyphylla, Adenophora triphylla. The silkworm and food material combined extract has a relatively high proliferation ability of mouse splenocyte proliferation when the silkworm and food materials are used as a single material. In particularly, combined extract of silkworm and Cinnamomum cassia was stimulate cytokine production on T lymphocyte such as IL12, $IFN-{\gamma}$. Combined extract of silkworm and Liriope platyphylla was stimulate cytokine production on T lymphocyte such as IL2, IL4, IL10. Conclusion : In conclusion, the combined extract of the silkworm and Cinnamomum cassia or Liriope platyphylla may enhance immune function by regulating mouse splenocyte proliferation and stimulating cytokine production.

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