한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제21권2호
  • /
  • Pages.167-180
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  • 2017
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Involvement of PI3K and MMP1 in PDGF-induced Migration of Human Adipose-derived Stem Cells

  • Lim, Yoonhwa (Dept. of Biotechnology, Seoul Women's University) ;
  • Lee, Minji (Dept. of Biotechnology, Seoul Women's University) ;
  • Jeong, Hyeju (Dept. of Biotechnology, Seoul Women's University) ;
  • Kim, Haekwon (Dept. of Biotechnology, Seoul Women's University)
  • 투고 : 2017.04.28
  • 심사 : 2017.05.11
  • 발행 : 2017.06.30
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초록

Human adult stem cells have widely been examined for their clinical application including their wound healing effect in vivo. To function as therapeutic cells, however, cells must represent the ability of directed migration in response to signals. This study aimed to investigate the mechanism of platelet-derived growth factor (PDGF)-induced migration of the human abdominal adipose-derived stem cells (hADSCs) in vitro. A general matrix metalloproteinase (MMP) inhibitor or a MMP2 inhibitor significantly inhibited the PDGF-induced migration. PDGF treatment exhibited greater mRNA level and denser protein level of MMP1. The conditioned medium of PDGF-treated cells showed a caseinolytic activity of MMP1. Transfection of cells with siRNA against MMP1 significantly inhibited MMP1 expression, its caseinolytic activity, and cell migration following PDGF treatment. Phosphatidylinositol 3-kinase (PI3K) inhibitor reduced the migration by about 50% without affecting ERK and MLC proteins. Rho-associated protein kinase inhibitor mostly abolished the migration and MLC proteins. The results suggest that PDGF might signal hADSCs through PI3K, and MMP1 activity could play an important role in this PDGF-induced migration in vitro.

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참고문헌

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