Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Glucosinolate and isothiocyabate contents according to processing of Kimchi cabbage (Brassica rapa L. ssp. pekinensis)

배추의 가공에 따른 glucosinolates 및 isothiocyanates 함량 변화

  • Jang, Miran (Plant Resources Research Institute Duksung Women's University) ;
  • Kim, Gun-Hee (Plant Resources Research Institute Duksung Women's University)
  • 장미란 (덕성여자대학교 식물자원연구소) ;
  • 김건희 (덕성여자대학교 식물자원연구소)
  • Received : 2016.12.19
  • Accepted : 2017.06.08
  • Published : 2017.06.30
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Abstract

This study examined the total glucosinolate (GSL) and isothiocyanate (ITC) contents according to different processing conditions; fresh Kimchi cabbage (Brassica rapa L. ssp. pekinensis), salted Kimchi cabbage and kimchi (storage temperature $4^{\circ}C$ and $20^{\circ}C$) using two different cultivars (Bomatnorang and Chunkwang). Four GSL peaks representing gluconapin, glucobrassicanapin, glucobrassicin and 4-methoxyglucobrassicin were detected in Kimchi cabbage by HPLC and HPLC/MS analysis. The total GSL contents of fresh Kimchi cabbage of Bomatnorang and Chunkwang were $21.37{\pm}1.06{\mu}g/g$ dry weight (DW) and $20.96{\pm}3.33{\mu}g/g$ DW, respectively. After salting, the total GSL contents of salted Kimchi cabbage decreased by 39% and 52% in Bomatnorang and Chunkwang, respectively. Finally, the total GSL contents of kimchi after storage at $20^{\circ}C$ decreased by 83% and 56% in Bomatnorang and Chunkwang, respectively. The extracted ITC contents were analyzed by GC/MS. Three ITC peaks were detected in Kimchi cabbage representing 2-phenylethyl ITC, 3-butenyl ITC and 4-pentenyl ITC. The 2-phenylethyl ITC levels increased during the salting process but this generally fell during storage at $20^{\circ}C$ as kimchi. The 3-butenyl ITC levels of Kimchi cabbage according to processing decreased rapidly due to salting and then decreased slowly during storage as kimchi. The 4-pentenyl ITC of Kimchi cabbage was lost during the salting process. The results for the change in GSL and ITC contents during the kimchi making process will be used in the food industry.

본 연구는 두 품종 배추(봄맛노랑 및 춘광)의 가공조건(신선배추, 절임배추, $4^{\circ}C$ 저장 김치 및 $20^{\circ}C$ 저장 김치)에 따른 GSL 및 ITC의 정성 정량 분석을 하였다. HPLC와 HPLC/MS분석 결과 봄맛노랑 및 춘광 배추에는 4종류의 GSL(sinigrin, gluconapin, glucobrassicanapin, 4-methoxyglucobrassicin)가 확인되었다. 신선배추의 총 GSL 함량은 봄맛노랑; $21.37{\pm}1.06{\mu}g/g$ DW, 춘광; $20.96{\pm}3.33{\mu}g/g$ DW에서 절임공정을 거쳐 봄맛노랑, 춘광이 각각 39%, 52% 감소하였다. 또한 김치로 가공하여 $20^{\circ}C$에 저장하였을 때 신선배추 대비 봄맛노랑, 춘광이 각각 83%, 56% 감소하여 GSL의 함량은 절임공정 및 숙성과정에서 크게 소실되는 것으로 나타났다. 또한 GC/MS분석 결과 봄맛노랑 및 춘광 배추에는 3종류의 ITC(2-phenylethyl ITC, 3-butenyl ITC, 4-pentenyl ITC)가 확인되었다. 2-Phenylethyl ITC는 봄맛노랑, 춘광에서 절임공정에 의하여 증가하였다가 $20^{\circ}C$에 저장하며 숙성되는 동안 다량 소실되었다. 3-Butenyl ITC은 절임공정에서부터 감소하는 경향을 나타냈으며, 김치로 저장하는 동안에는 크게 감소하지 않았다. 4-Pentenyl ITC는 신선배추에서만 발견되었다가 가공처리에 의해 완전히 소실되어 관찰되지 않았다. 본 연구는 향후 산업체에서 배추 가공식품의 기초자료로서 활용할 수 있을 것이다.

Keywords

References

  1. Kim CR, Lim YS, Lee SW, Kim SJ (2011) Identification and quantification of glucosinolates in rocket salad(Eruca sativa). CNU J Agric Sci, 38, 285-294
  2. Fenwick GR, Heaney RK, Mullin WJ (1983) Glucosinolates and their break down products in food and food plants. Crit Rev Food Sci Nutr, 18, 123-201 https://doi.org/10.1080/10408398209527361
  3. Bennett RN, Mellon FA, Botting NP, Eagles J, Rosa EA, Williamson G (2002) Identification of the major glucosinolate (4-mercaptobutyl glucosinolate) in leaves of Eruca sativa L. (salad roket). Phytochemistry, 61, 25-30 https://doi.org/10.1016/S0031-9422(02)00203-0
  4. Jang M, Hong E, Kim GH (2010) Evaluation of antibacterial activity of 3-butenyl, 4-pentenyl, 2-phenylethyl, and benzyl isothiocyanate in Brassica Vegetables. J Food Sci, 75, M412-M416 https://doi.org/10.1111/j.1750-3841.2010.01725.x
  5. Bennet RN, Wallgsrove RM (1994) Secondary metabolites in plant defence mechanisms. New Phytol, 127, 617-633 https://doi.org/10.1111/j.1469-8137.1994.tb02968.x
  6. Fahey JW, Zhang Y, Talalay P (1997) Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proc Natl Acad Sci USA, 94, 10367-10372 https://doi.org/10.1073/pnas.94.19.10367
  7. Smith TK, Mithen R, Johnson IT (2003) Effects of brassica vegetable juice on the induction of apoptosis and aberrant crypt foci in rat colonic mucosal crypts in vivo. Carcinogenesis, 24, 491-495 https://doi.org/10.1093/carcin/24.3.491
  8. Park JH, Lee SJ, Kim BR, Woo ET, Lee JS, Han EH, Lee YH, Park YD (2011) Isolation of myrosinase and glutathione s-transferase genes and transformation of these genes to develop phenylethyl isothiocyanate enriching Chinese cabbage. Kor J Hort Sci Technol, 29, 623-632
  9. Hwang ES (2010) Changes in Myrosinase activity and total glucosinolate levels in Korean Chinese Cabbages by salting conditions. Korean J Food Cookery Sci, 26, 104-109
  10. Kim MK, Hong EY, Kim GH (2010) Change of total glucosinolates Level according to Processing Treatments in Chinese Cabbage (Brassica campestris L. ssp. Pekinensis) from Different Harvest Seasons. Kor J Hort Sci Technol, 28, 593-599
  11. Jung JI, Hong EY, Kim MK, Jung JW, Oh JY, Kwon MS, Lee KP, Kim GH (2009) Changes in total glucosinolates levels and physico-chemical properties of kimchi using Korean Chinese Cabbage of harvest time according to various storage conditions. Korean J Food Preserv, 16, 612-617
  12. Shim YH, Ah GJ, Yoo CH (2003) Characterization of salted Chinese cabbage in relation to salt content, temperature and time. Korean J Soc Food Cookery Sci, 19, 210-215
  13. Hong EY, Kim GH (2006) Changes in isothiocyanate levels in Korean Chinese cabbage leaves during kimchi storage. Food Sci Biotechnol, 15, 688-693
  14. Al-Gendy AA, Lockwood GB (2003) GC-MS analysis of volatile hydrolysis products from glucosinolates in Farsetia aegyptia var. ovalis. Flavour Fragrance J, 18, 148-152 https://doi.org/10.1002/ffj.1163
  15. You Y, Wu Y, Mao J, Zou L, Liu S (2008) Screening of Chinese brassica species for anti-cancer sulforaphane and erucin. Afr J Biotechnol, 7, 147-152
  16. Hwang ES, Hong EY, Kim GH (2012) Determination of bioactive compounds and anti-cancer effect from extracts of Korean Cabbage and Cabbage. Korean J Food Nutr, 25, 259-265 https://doi.org/10.9799/ksfan.2012.25.2.259
  17. Tyagi AK (2002) Influence of water soaking of mustard cake on glucosinolates (thioglucosinolates) in foods and Feeds. Animl Feed Sci Technol, 99, 215-219 https://doi.org/10.1016/S0377-8401(02)00142-6
  18. Chun SS, Choi OJ, Cho YS, Park SK, Park JR (1995) Changes in pungent components of Dolsan leaf mustard kimchi during fermentation. J Korean Soc Food Nutr, 24, 54-59
  19. Park JR, Park SK, Cho YS, Chun SS (1994) Purification and characterization of myrosinase in Dolsan leaf mustard (Brassica juncea ) and changes in myrosinase activity during fermentation of leaf mustard kimchi. J Korean Soc Food Cult, 9, 137-142
  20. Foo HL, Gronning LM, Goodenough L, Bones AM, Danielsen B, Whiting DA, Rossiter JT (2000) Purification and characterization of epithiospecifier protein from Brassica napus: enzymic intra molecular sulfur addition within alkenyl thiohydroximates derived from alkenyl glucosinolate hydrolysis. FEBS Lett, 468, 243-246 https://doi.org/10.1016/S0014-5793(00)01176-5
  21. Kim HR, Cho KJ, Kim JS, Lee IS (2006) Quality changes of mustard leaf (dolsangat) kimchi during low temperature storage. Korean J Food Sci Technol, 38, 609-614
  22. Pyo YH, Kim JS, Hahn YS (2000) Volatile compounds of mustard leaf (Brassica juncea) kimchi and their changes during fermentation. Korean J Food Sci Technol, 32, 56-61
  23. Lee KH, Kuack HS, Jung JW, Lee EJ, Jeong DM, Kang KY, Chae KI, Yun SH, Jang MR, Cho SD, Kim GH, Oh JY (2013) Comparison of the quality characteristics between spring cultivars of Kimchi cabbage (Brassica rapa L. ssp. pekinensis). Korean J Food Preserv, 20, 182-190 https://doi.org/10.11002/kjfp.2013.20.2.182

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