Mass Spectrometry Letters

Korean Society for Mass Spectrometry (한국질량분석학회)
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Simple and Rapid Liquid Chromatography-Tandem Mass Spectrometry Analysis of Arctigenin and its Application to a Pharmacokinetic Study

  • Thapa, Subindra Kazi (College of Pharmacy, Wonkwang University) ;
  • Weon, Kwon-Yeon (College of Pharmacy, Catholic University of Daegu) ;
  • Jeong, Seok Won (College of Pharmacy, Catholic University of Daegu) ;
  • Kim, Tae Hwan (Center for Pharmacometrics and Systems Pharmacology, Department of Pharmaceutics, College of Pharmacy, University of Florida) ;
  • Upadhyay, Mahesh (College of Pharmacy, Wonkwang University) ;
  • Han, Yo-Han (College of Pharmacy, Wonkwang University) ;
  • Jin, Jong-Sik (Department of Oriental Medicine Resources, Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Hong, Seung-Heon (College of Pharmacy, Wonkwang University) ;
  • Youn, Yu Seok (School of Pharmacy, Sungkyunkwan University) ;
  • Shin, Beom Soo (School of Pharmacy, Sungkyunkwan University) ;
  • Shin, Soyoung (College of Pharmacy, Wonkwang University)
  • Received : 2017.06.01
  • Accepted : 2017.06.13
  • Published : 2017.06.30
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Abstract

Arctigenin is the main active ingredient of Fructus Arctii, which has been reported with a variety of therapeutic activities including anti-cancer, anti-inflammation, anti-virus, and anti-obesity effects. In this study, a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of arctigenin in rat plasma. The assay utilized a simple protein precipitation with methanol and the mobile phase consisted of 100% methanol and water containing 0.1% formic acid (65:35 v/v). Arctigenin and the internal standard (psoralen) were monitored using a positive electrospray turbo ionspray mode with multiple reaction monitoring transitions of m/z $373.2{\rightarrow}136.9$ and m/z $187.2{\rightarrow}130.9$, respectively, and total chromatographic run time was within 5 min. The lower limit of quantification (LLOQ) of arctigenin was 5 ng/mL in the rat plasma. The intra- and inter-day accuracy of arctigenin at LLOQ and matrix-matched quality control samples ranged 97.4 - 104.8% and 97.2 - 102.0%, respectively. The intra-day precision was within 4.80% and the inter-day precision was within 5.92%. Application of the present method was demonstrated through a pharmacokinetic study after intravenous and oral administration of arctigenin in male Sprague Dawley rats.

Keywords

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