Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Physicochemical Properties of Meat Batter Added with Edible Silkworm Pupae (Bombyx mori) and Transglutaminase

  • Park, Yoo-Sun (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Choi, Yun-Sang (Food Processing Research Center, Korean Food Research Institute) ;
  • Hwang, Ko-Eun (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Kim, Tae-Kyung (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Lee, Cheol-Won (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Shin, Dong-Min (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Han, Sung Gu (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
  • Received : 2017.05.16
  • Accepted : 2017.05.28
  • Published : 2017.06.30
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Abstract

This study was conducted to investigate the physicochemical properties of meat batters prepared with fresh pork meat, back fat, water, and salt and formulated with three different amounts (5%, 10%, and 15%) of silkworm pupae (Bombyx mori) powder and transglutaminase (TG). Meat batters formulated with silkworm pupae powder showed significantly higher contents of protein and ash than control batter. Addition of silkworm pupae to batter also showed significantly lower cooking loss than the control. Moreover, meat batter containing 15% silkworm pupae showed no significant difference in redness value compared to the control. In addition, pH, viscosity, hardness, gumminess, and chewiness were improved after the addition of silkworm pupae. Furthermore, meat batter formulated with TG and silkworm pupae showed improved hardness, gumminess, chewiness and viscosity compared to control batter. Addition of 1% TG with 15% silkworm pupae to meat batter resulted in significantly higher pH, textures, and viscosity. Our data suggest that both silkworm pupae and TG can be added to meat batter to improve its physicochemical properties. Therefore, combination of silkworm pupae and TG could be a new nutritional and functional source for meat products.

Keywords

References

  1. AOAC. (1995) Official methods of analysis of AOAC (Vol. 41, 16th ed.) Association of Official Analytical Chemists, Washington, DC.
  2. Assielou, B., Due, E. A., Koffi, M. D., Dabonne, S., and Kouame, P. L. (2015) Oryctes owariensis larvae as good alternative protein source: Nutritional and functional properties. Ann. Res. Rev. Biol. 8, 1-9.
  3. Atilgan, E. and Kilic, B. (2017) Effects of microbial transglutaminase, fibrimex and alginate on physicochemical properties of cooked ground meat with reduced salt level. J. Food Sci. Technol. 54, 303-312. https://doi.org/10.1007/s13197-016-2463-x
  4. Baker, M. A., Shin, J. T., and Kim, Y. W. (2016) An exploration and investigation of edible insect consumption: The impacts of image and description on risk perceptions and purchase intent. Psychol. Mark. 33, 94-112. https://doi.org/10.1002/mar.20847
  5. Bourne, M. C. (2002) Food Texture and Viscosity: Concept and Measurement (2nd ed.). New York: Academic Press.
  6. Chin, K. B., Go, M. Y., and Xiong, Y. L. (2009) Konjac flour improved textural and water retention properties of transglutaminase-mediated, heat-induced porcine myofibrillar protein gel: Effect of salt level and transglutaminase incubation. Meat Sci. 81, 565-572. https://doi.org/10.1016/j.meatsci.2008.10.012
  7. Choe, J. H., Kim, H. Y., Lee, J. M., Kim, Y. J., and Kim, C. J. (2013) Quality of frankfurter-type sausages with added pig skin and wheat fiber mixture as fat replacers. Meat Sci. 93, 849-854. https://doi.org/10.1016/j.meatsci.2012.11.054
  8. Choi, Y. S., Choi, J. H., Han, D. J., Kim, H. Y., Kim, H. W., Lee, M. A., Chung, H. J., and Kim, C. J. (2012) Effects of Laminaria japonica on the physico-chemical and sensory characteristics of reduced-fat pork patties. Meat Sci. 91, 1-7. https://doi.org/10.1016/j.meatsci.2011.11.011
  9. Choi, Y. S., Jeon, K. H., Park, J. D., Sung, J. M., Seo, D. H., Ku, S. K., Oh, N. S., and Kim, Y. B. (2015) Comparison of pork patty quality characteristics with various binding agents. Korean J. Food Cook. Sci. 31, 588-595. https://doi.org/10.9724/kfcs.2015.31.5.588
  10. Choi, Y. S., Jeong, T. J., Kim, H. W., Hwang, K. E., Sung, J. M., Seo, D. H., Kim, Y. B., and Kim, C. J. (2016) Combined effects of sea mustard and transglutaminase on the quality characteristics of reduced-salt frankfurters. J. Food Process. Preserv. DOI:10.1111/jfpp.12945
  11. Dimitrakopoulou, M. A., Ambrosiadis, J. A., Zetou, F. K., and Bloukas, J. G. (2005) Effect of salt and transglutaminase (TG) level and processing conditions on quality characteristics of phosphate-free, cooked, restructured pork shoulder. Meat Sci. 70, 743-749. https://doi.org/10.1016/j.meatsci.2005.03.011
  12. Gujral, H. S. and Rosell, C. M. (2004) Functionality of rice flour modified with a microbial transglutaminase. J. Cereal Sci. 39, 225-230. https://doi.org/10.1016/j.jcs.2003.10.004
  13. Herrero, A. M., Carmona, P., Cofrades, S., and Jimenez-Colmenero, F. (2008) Raman spectroscopic determination of structural changes in meat batters upon soy protein addition and heat treatment. Food Res. Int. 41, 765-772. https://doi.org/10.1016/j.foodres.2008.06.001
  14. Hong, G. P., Min, S. G., and Chin, K. B. (2012) Emulsion properties of pork myofibrillar protein in combination with microbial transglutaminase and calcium alginate under various pH conditions. Meat Sci. 90, 185-193. https://doi.org/10.1016/j.meatsci.2011.06.023
  15. Hwang, S. Y. and Choi, S. K. (2015) Quality characteristics of muffins containing mealworm (Tenebrio molitor). Korean J. Culinary Res. 21, 104-115.
  16. Ionescu, A., Aprodu, I., Daraba, A., and Porneala, L. (2008) The effects of transglutaminase on the functional properties of the myofibrillar protein concentrate obtained from beef heart. Meat Sci. 79, 278-284. https://doi.org/10.1016/j.meatsci.2007.09.011
  17. Jeon, J. R. and Park, J. R. (1992) Functional properties of silkworm larvae protein concentrate after enzyme treatments. J. Korean Soc. Food Sci. Nutr. 21, 706-711.
  18. Kim, H. M., Kim, J. N., Kim, J. S., Jeong, M. Y., Yun, E. Y., Hwang, J. S., and Kim, A. J. (2015) Quality characteristics of patty prepared with mealworm powder. Korean J. Food Nutr. 28, 813-820. https://doi.org/10.9799/ksfan.2015.28.5.813
  19. Kim, H. W., Setyabrata, D., Lee, Y. J., Jones, O. G., and Kim, Y. H. B. (2016) Pre-treated mealworm larvae and silkworm pupae as a novel protein ingredient in emulsion sausages. Innov. Food Sci. Emerg. Technol. 38, 116-123. https://doi.org/10.1016/j.ifset.2016.09.023
  20. Kim, H. Y., Lee, E. S., Jeong, J. Y., Choi, J. H., Choi, Y. S., Han, D. J., Lee, M. A, Kim, S. Y., and Kim, C. J. (2010) Effect of bamboo salt on the physicochemical properties of meat emulsion systems. Meat Sci. 86, 960-965. https://doi.org/10.1016/j.meatsci.2010.08.001
  21. Kim, I. S., Jin, S. K., Jo, C. U., Lee, M. H., and Jang, A. R. (2008) Quality characteristics of pork patties containing silkworm powder and vegetable worm (Paecilomyces japonica) during cold storage. Korean J. Food Sci. Anim. Resour. 28, 521-528. https://doi.org/10.5851/kosfa.2008.28.5.521
  22. Kim, S. H., Kim, K. B., Noh, J. S., Yun, E. Y., and Choi, S. K. (2014) Quality characteristics of pasta with addition of mealworm (Tenebrio molitor). Food Serv. Ind. J. 10, 55-64.
  23. Kinyuru, J. N., Kenji, G. M., and Njoroge, M. S. (2009) Process development, nutrition and sensory qualities of wheat buns enriched with edible termites (Macrotermes subhylanus) from Lake Victoria region, Kenya. AJFAND. 9, 1739-1750.
  24. Lee, H. C. and Chin., K. B. (2009) Effect of transglutaminase, acorn, and mungbean powder on quality characteristics of low-fat/salt pork model sausages. Korean J. Food Sci. 29, 374-381. https://doi.org/10.5851/kosfa.2009.29.3.374
  25. Lin, S., Huff, H. E., and Hsieh, F. (2000) Texture and chemical characteristics of soy protein meat analog extruded at high moisture. J. Food Sci. 65, 264-269. https://doi.org/10.1111/j.1365-2621.2000.tb15991.x
  26. Meiying, Z., Guocheng, D., and Jian, C. (2002) pH control strategy of batch microbial transglutaminase production with Streptoverticillium mobaraense. Enzyme Microb. Technol. 31, 477-481. https://doi.org/10.1016/S0141-0229(02)00127-8
  27. Min, K. T., Kang, M. S., Kim, M. J., Lee, S. H., Han, J. S., and Kim, A. J. (2016) Manufacture and quality evaluation of cookies prepared with mealworm (Tenebrio molitor) powder. Korean J. Food Nutr. 29, 12-18. https://doi.org/10.9799/ksfan.2016.29.1.012
  28. Motoki, M. and Seguro, K. (1998) Transglutaminase and its use for food processing. Trends Food Sci. Technol. 9, 204-210. https://doi.org/10.1016/S0924-2244(98)00038-7
  29. Muguruma, M., Tsuruoka, K., Katayama, K., Erwanto, Y., Kawahara, S., Yamauchi, K., Sathe, S. K., and Soeda, T. (2003) Soybean and milk proteins modified by transglutaminase improves chicken sausage texture even at reduced levels of phosphate. Meat Sci. 63, 191-197. https://doi.org/10.1016/S0309-1740(02)00070-0
  30. Nowak, V., Persijn, D., Rittenschober, D., and Charrondiere, U. R. (2016) Review of food composition data for edible insects. Food Chem. 193, 39-46. https://doi.org/10.1016/j.foodchem.2014.10.114
  31. Park, G. S. and Park, J. R. (1986) Functional properties of silkworm larvae protein concentrate. Korean J. Food Sci. Technol. 18, 204-209.
  32. Pietrasik, Z. and Duda, Z. (2000) Effect of fat content and soy protein/carrageenan mix on the quality characteristics of comminuted, scalded sausages. Meat Sci. 56, 181-188. https://doi.org/10.1016/S0309-1740(00)00038-3
  33. Pietrasik, Z., Jarmoluk, A., and Shand, P. J. (2007) Effect of non-meat proteins on hydration and textural properties of pork meat gels enhanced with microbial transglutaminase. LWT-Food Sci. Technol. 40, 915-920. https://doi.org/10.1016/j.lwt.2006.03.003
  34. Rosell, C. M., Wang, J., Aja, S., Bean, S., and Lookhart, G. (2003) Wheat flour proteins as affected by transglutaminase and glucose oxidase. Cereal Chem. 80, 52-55. https://doi.org/10.1094/CCHEM.2003.80.1.52
  35. Rumpold, B. A. and Schluter, O. K. (2013) Potential and challenges of insects as an innovative source for food and feed production. Innov. Food Sci. Emerg. Technol. 17, 1-11. https://doi.org/10.1016/j.ifset.2012.11.005
  36. Sah, L. P. and Jung, C. E. (2012) Global perspective of edible insects as human food. Korean J. Soil Zool. 16, 1-8.
  37. Steenkamp, J. B. E. and van Trijp, H. (1996) Quality guidance: A consumer-based approach to food quality improvement using partial least squares. Eur. Rev. Agric. Econ. 23, 195-215. https://doi.org/10.1093/erae/23.2.195
  38. Stevenson, C. D., Dykstra, M. J., and Lanier, T. C. (2013) Capillary pressure as related to water holding in polyacrylamide and chicken protein gels. J. Food Sci. 78, 145-151. https://doi.org/10.1111/1750-3841.12036
  39. Sugumaran, M. (2002) Comparative biochemistry of eumelanogenesis and the protective roles of phenoloxidase and melanin in insects. Pigm. Cell Res. 15, 2-9. https://doi.org/10.1034/j.1600-0749.2002.00056.x
  40. Tan, H. S. G., Verbaan, Y. T., and Stieger, M. (2016) How will better products improve the sensory-liking and willingness to buy insect-based foods? Food Res. Int. 92, 95-105.
  41. Tseng, T. F., Liu, D. C., and Chen, M. T. (2000) Evaluation of transglutaminase on the quality of low-salt chicken meat-balls. Meat Sci. 55, 427-431. https://doi.org/10.1016/S0309-1740(99)00172-2
  42. Zhou, J. and Han, D. (2006) Proximate, amino acid and mineral composition of pupae of the silkworm Antheraea pernyi in China. J. Food Compost. Anal. 19, 850-853. https://doi.org/10.1016/j.jfca.2006.04.008
  43. Zorba, O., Kurt, S., and Genccelep, H. (2005) The effects of different levels of skim milk powder and whey powder on apparent yield stress and density of different meat emulsions. Food Hydrocoll. 19, 149-155. https://doi.org/10.1016/j.foodhyd.2004.05.001

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