Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Complete genome sequence of a novel bacteriophage SPG24 isolated from Cheonggukjang

청국장에서 분리된 신생 박테리오파지 SPG24의 전체 염기 서열

  • Kim, Chaehyun (Department of Microbiology, College of Natural Sciences, Chungbuk National University) ;
  • Lee, Gyu-Cheol (Department of Microbiology, Water Quality Research Center, K-water) ;
  • Kim, In Kyo (Department of Microbiology, College of Natural Sciences, Chungbuk National University) ;
  • Kim, Seok Cheon (Department of Microbiology, College of Natural Sciences, Chungbuk National University) ;
  • Lee, Oh Hyung (Department of Bio Sciences, College of Natural Science, Mokpo National University) ;
  • Lee, Chan Hee (Department of Microbiology, College of Natural Sciences, Chungbuk National University)
  • 김채현 (충북대학교 자연과학대학 미생물학과) ;
  • 이규철 (한국수자원공사 수질연구센터 미생물과) ;
  • 김인교 (충북대학교 자연과학대학 미생물학과) ;
  • 김석천 (충북대학교 자연과학대학 미생물학과) ;
  • 이오형 (목포대학교 자연과학대학 생명과학과) ;
  • 이찬희 (충북대학교 자연과학대학 미생물학과)
  • Received : 2017.06.08
  • Accepted : 2017.06.08
  • Published : 2017.06.30
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Abstract

A Bacillus subtilis strain, designated G24, was isolated from grape during a study on the fermentation of Cheongkukjang, Korean traditional fast-fermented bean paste. Also, a newly isolated bacteriophage SPG24, which was found to inhibit the fermentation process, uses B. subtilis G24 as a host. The complete genome sequence of the bacteriophage SPG24 was 152,060 bp in length, with a G+C content of 42.2%. This sequence included 232 ORF; 58 forward ORFs and 174 reverse ORFs.

Bacillus subtilis를 이용하여 청국장 발효 연구를 진행하던 중 발효능이 뛰어난 B. subtilis G24라는 균주를 새로 분리하였고, 이를 숙주로 이용하는 bacteriophage SPG24를 새로 분리하였다. 본 연구에서는 bacteriophage SPG24의 유전체 서열을 해독하였으며 전체길이 152,060 bp, G+C 함량 42.2%, 232개 ORF의 bacteriophage SPG24는 B. subtilis G24를 숙주로 이용함으로써 청국장 발효를 저해하는 것으로 확인되었다.

Keywords

References

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