The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Effects of Garlic on Uncoupling Protein 2 (UCP2) Transcriptional Regulation in Metabolic Tissues of UCP2 Transgenic Mice Fed on a High-Fat Diet

마늘이 고지방 식이를 섭취한 UCP2 형질전환 마우스의 대사성 조직에서 UCP2 전사 조절에 미치는 영향

  • Lee, Mak-Soon (Dept. of Nutritional Science and Food Management, Ewha Womans University) ;
  • Lee, Seohyun (Dept. of Nutritional Science and Food Management, Ewha Womans University) ;
  • Shin, Yoonjin (Dept. of Nutritional Science and Food Management, Ewha Womans University) ;
  • Jung, Sunyoon (Dept. of Nutritional Science and Food Management, Ewha Womans University) ;
  • Park, Seonyoung (Dept. of Nutritional Science and Food Management, Ewha Womans University) ;
  • Kim, Yangha (Dept. of Nutritional Science and Food Management, Ewha Womans University)
  • 이막순 (이화여자대학교 식품영양학과) ;
  • 이서현 (이화여자대학교 식품영양학과) ;
  • 신윤진 (이화여자대학교 식품영양학과) ;
  • 정선윤 (이화여자대학교 식품영양학과) ;
  • 박선영 (이화여자대학교 식품영양학과) ;
  • 김양하 (이화여자대학교 식품영양학과)
  • Received : 2017.04.20
  • Accepted : 2017.05.26
  • Published : 2017.06.30
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Abstract

This study was performed to investigate the effects of garlic on uncoupling protein 2 (UCP2) transcriptional regulation of UCP2-luciferase transgenic mice fed on a high fat diet to induce obesity. To examine the transcriptional regulation of UCP2, we generated transgenic mice with a UCP2 promoter (-1,830/+30 bp) containing luciferase as a reporter gene. UCP2-luciferase transgenic mice were fed a 45% high-fat diet for 8 weeks to induce obesity. Subsequently, mice were maintained on either a high-fat control diet (TG-CON), or high-fat diets supplemented with 2% (TG-GL2) or 5% (TG-GL5) garlic for a further 8 weeks. Dietary garlic reduced body weight and energy efficiency ratio in the TG-GL5 group, compared to the TG-CON group. Furthermore, garlic supplementation significantly decreased white adipose tissue fat mass and plasma levels of triglycerides, total cholesterol, and leptin in the TG-GL2 and TG-GL5 groups, compared to the TG-CON group. Specifically, UCP2 promoter activity in metabolic tissues such as liver, white adipose tissue, brown adipose tissue, and skeletal muscle was increased by garlic supplementation. These results suggest that dietary garlic was partially associated with an increase of UCP2 transcriptional activity in metabolic tissues for decreasing obesity.

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References

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