Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Analysis of Semen Parameters in α1,3-Galactosyltransferase-/- Boars

  • Hwang, In-Sul (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Lee, Seung-Chan (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Kim, Sung Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kwon, Dae-Jin (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Park, Mi-Ryung (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Yang, Hyeon (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Ock, Sun-A (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Woo, Jae-Seok (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Im, Gi-Sun (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Hwang, Seongsoo (Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • Received : 2017.03.18
  • Accepted : 2017.06.05
  • Published : 2017.06.30
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Abstract

It is very difficult to get the information about semen quality analysis in transgenic pigs because of limited numbers and research facilities. Therefore, in the present study, we analyzed the semen quality of transgenic boars generated for xenotransplantation research. Briefly, the semen samples were collected from 5 homozygous ${\alpha}1,3$-Galactosyltransferase knock-out ($GalT^{-/-}$) transgenic boars and immediately transported to the laboratory. These semen samples were decupled with DPBS and conducted to analyze semen parameters by a computer-assisted semen analysis (CASA) system. The boar semen were examined all 12 parameters such as total motility (TM), curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), and hyperactivated (HYP), etc. In results, among the 5 $GalT^{-/-}$ boars, three boars (#134, 144, and 170) showed normal range of semen parameters, but #199 and 171 boars showed abnormal ranges of semen parameters according to standard ranges of semen parameters. Unfortunately, #171 boar showed azoospermia symptom with rare sperm counts in the original semen. Conclusively, assessment of semen parameters by CASA system is useful to pre-screening of reproductively healthy boar prior to natural mating and artificial insemination for multiplication and breeding.

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References

  1. Abouna GM. 2008. Organ Shortage Crisis: Problems and Possible Solutions. Transplantation Proceedings 40:34-38. https://doi.org/10.1016/j.transproceed.2007.11.067
  2. Amann RP, Waberski D. 2014. Computer-assisted sperm analysis (CASA): capabilities and potential developments. Theriogenology 81:5-17 e11-13. https://doi.org/10.1016/j.theriogenology.2013.09.004
  3. Berger T, Anderson DL, Penedo MCT. 1996. Porcine sperm fertilizing potential in relationship to sperm functional capacities. Anim. Reprod. Sci. 44:231-239. https://doi.org/10.1016/0378-4320(96)01565-5
  4. Budworth PR, Amann RP, Chapman PL. 1988. Relationships Between Computerized Measurements of Motion of Frozen-Thawed Bull Spermatozoa and Fertility. Journal of Andrology 9:41-54. https://doi.org/10.1002/j.1939-4640.1988.tb01007.x
  5. Choi MS, Shim MR, Oh MY, Kim KW, Lee HC, Yang BC, Chung HK, Kim JH, Lee HT, Hwang IS, Hochi S, Heo YT, Kim NH, Uhm SJ, Park JK, Chang WK, Chung HJ. 2012. Proteins associated with reproductive disorders in testes of human erythropoietin gene-harboring transgenic boars. Theriogenology 78:1020-1029. https://doi.org/10.1016/j.theriogenology.2012.02.013
  6. Flowers WL. 1997. Management of boars for efficient semen production. Reprod. Fertil. 52(suppl.): 67-78.
  7. Gil MC, Garcia-Herreros M, Baron FJ, Aparicio IM, Santos AJ, Garcia-Marin LJ. 2009. Morphometry of porcine spermatozoa and its functional significance in relation with the motility parameters in fresh semen. Theriogenology 71:254-263. https://doi.org/10.1016/j.theriogenology.2008.07.007
  8. Hirano Y, Shibahara H, Obara H, Suzuki T, Takamizawa S, Yamaguchi C, Tsunoda H, Sato I. 2001. Relationships between sperm motility characteristics assessed by the computer-aided sperm analysis (CASA) and fertilization rates in vitro. J. Assist. Reprod. Genet. 18:213-218.
  9. Holt C, Holt WV, Moore HD, Reed HC, Curnock RM. 1997. Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations: results of two fertility trials. J. Androl. 18:312-323
  10. Hwang S, Oh KB, Kim DH, Woo JS, Shim H, Yun IJ, Park JK, Im GS. 2012. Production of ${\alpha}1$,3-Galactosyltransferase (GalT) Double Knock-out (-/-)Transgenic Pigs for Xenotransplantation. Journal of Embryo Transfer 27:9-14.
  11. Hwang S, Oh KB, Kwon DJ, Ock SA, Lee JW, Im GS, Lee SS, Lee K, Park JK. 2013. Improvement of cloning efficiency in minipigs using post-thawed donor cells treated with roscovitine. Mol. Biotechnol. 55:212-216. https://doi.org/10.1007/s12033-013-9671-7
  12. Jung M, Rudiger K, Schulze M. 2015. In vitro measures for assessing boar semen fertility. Reprod. Domest. Anim. 50(suppl. 2):20-24.
  13. Kwon D-J, Kim D-H, Hwang I-S, Kim D-E, Kim H-J, Kim J-S, Lee K, Im G-S, Lee J-W, Hwang S. 2016. Generation of $\alpha$-1,3-galactosyltransferase knocked-out transgenic cloned pigs with knocked-in five human genes. Transgenic Research 1-11.
  14. Lai L, Kolber-Simonds D, Park KW, Cheong HT, Greenstein JL, Im GS, Samuel M, Bonk A, Rieke A, Day BN, Murphy CN, Carter DB, Hawley RJ, Prather RS. 2002. Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295:1089-1092. https://doi.org/10.1126/science.1068228
  15. Lavara R, Moce E, Lavara F, Viudes de Castro MP, Vicente JS. 2005. Do parameters of seminal quality correlate with the results of on-farm inseminations in rabbits? Theriogenology 64:1130-1141. https://doi.org/10.1016/j.theriogenology.2005.01.009
  16. Lee HJ, Lee BC, Kim YH, Paik NW, Rho HM. 2011. Characterization of transgenic pigs that express human decay accelerating factor and cell membrane-tethered human tissue factor pathway inhibitor. Reprod. Domest. Anim. 46:325-332. https://doi.org/10.1111/j.1439-0531.2010.01670.x
  17. Lee HG, Lee HC, Chung HJ, Hwang IS, Choi MS, Byun SJ, Kim MJ, Woo JS, Chang WK, Lee P, Lee HT, Park JK. 2008 Study on the reproductive function in transgenic pig harboring human erythropoietin (hEPO) gene. Reprod. Dev. Biol. 32:117-121.
  18. Park CG, Kim S, Lee P, Han JH, Lee HG, Byun SJ, Yang BS, Lee CH, Lee HT, Chang WK, Park JK. 2006. Sperm fertility of transgenic boar harboring hEPO gene is decreased. Reprod. Dev. Biol. 30:27-34
  19. Samper JC, Hellander JC, Crabo BG. 1991. Relationship between the fertility of fresh and frozen stallion semen and semen quality. J. Reprod. Fertil. Suppl. 44:107-114.
  20. Verstegen J, Iguer-Ouada M, Onclin K. 2002. Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology 57:149-179. https://doi.org/10.1016/S0093-691X(01)00664-1
  21. Vyt P, Maes D, Quinten C, Rijsselaere T, Deley W, Aerts M, Kruif A, van Soom A. 2008. Detailed motility examination of porcine semen and its predictive value towards reproductive performance in sows. Vlaams Diergeneeskd. Tijdschr. 77:291-298.
  22. Yamada K, Yazawa K, Shimizu A, Iwanaga T, Hisashi Y, Nuhn M, O'Malley P, Nobori S, Vagefi PA, Patience C, Fishman J, Cooper DK, Hawley RJ, Greenstein J, Schuurman HJ, Awwad M, Sykes M, Sachs DH. 2005. Marked prolongation of porcine renal xenograft survival in baboons through the use of alpha1,3-galactosyltransferase gene-knockout donors and the cotransplantation of vascularized thymic tissue. Nat. Med. 11:32-34. https://doi.org/10.1038/nm1172