Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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The Effect of Barbaloin on LPS-stimulated Inflammatory Reaction in Mice Peritoneal Macrophages

  • Jeon, Yong-Deok (Department of Oriental Medicine Resources, Chonbuk National University) ;
  • Lee, Jong-Hyun (College of Pharmacy, Dongduk Women's University)
  • Received : 2017.04.29
  • Accepted : 2017.06.14
  • Published : 2017.06.30
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Abstract

Barbaloin is a major component of Aloe vera, which has been used for a laxative. Also, barbaloin is C-glucoside of aloe emodin anthrone which is founded in Aloe vera. Barbaloin has varieties of pharmacological activity such as inhibitory effects on inflammation, histamine release, cancer and microbial infection. But the effect of barbaloin on lipopolysaccharide (LPS)-stimulated macrophages has not been understood. In this study, we evaluated the effects of barbaloin against LPS-stimulated production of nitric oxide (NO), inflammatory cytokines and MAPKs activation in macrophage. We treated barbaloin (0.1, 1, 10, $100{\mu}M$) in LPS-stimulated mice peritoneal macrophage. Our results showed that barbaloin significantly inhibited production of NO and cytokines of tumor necrosis factor $(TNF)-{\alpha}$, interleukin (IL)-6, interleukin $(IL)-1{\beta}$ in LPS-stimulated peritoneal macrophage. Moreover, barbaloin inhibited the phosphorylation of ERK and JNK in a dose dependent manner. These results indicated that barbaloin could be useful for inflammatory diseases.

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References

  1. Ajizian, S.J., B.K. English and E.A. Meals. 1999. Specific inhibitors of p38 and extracellular signal regulated kinase mitogen-activated protein kinase pathway block inducible nitric oxide synthase and tumor necrosis factor accumulation in murine macrophages stimulated with lipopolysaccharide and interferon-gamma. J. Infect. Dis. 7(14):939-944.
  2. Barbara, B.J, M. Niemcewicz, J. Kocik, L. Jung, E. Skopinskarozewska and P. Skopinski. 2014. Oral administration of Aloe vera gel, antimicrobial and anti-inflammatory herbal remedy, stimulates cell-mediated immunity and antibody production in a mouse model. Cent. Eur. J. Immunol. 39(2):125-126.
  3. Chang, F., L.S. Steelman, J.T. Lee, J.G. Shelton, P.M. Navolani, W.L. Blalock, R.A. Franklin and J.A. McCubrey. 2003. Signal transduction mediated by the Ras/Raf/MEK/ERK pathway from cytokine receptors to transcription factors: Potential targeting for therapeutic intervention. Leukemia 17(7):1263-1293. https://doi.org/10.1038/sj.leu.2402945
  4. Chen, Y.Y., M.J. Hsu, C.Y. Hsieh, L.W. Lee, Z.C. Chen and J.R. Sheu. 2014. Andrographolide inhibits nuclear factor-kB activation through JNK-Akt-p65 signaling cascade in tumor necrosis factor-${\alpha}$-stimulated vascular smooth muscle cells. ScientificWorld Journal. Doi:10.1155/2014.130381.
  5. Hattori, M., T. Kanda, Y.Z. Shu, T. Akao, K. Kobashi and T. Namba. 1988. Metabolism of barbaloin by intestinal bacteria. Chem. Pharm. Bull. 36(11):4462-4466. https://doi.org/10.1248/cpb.36.4462
  6. Kaminska, B. 2005. MAPK signaling pathways as molecular targets for anti-inflammatory therapy from molecular mechanisms to therapeutic benefits. Biochim. Biophys. Acta. 1754(1-2):253-262. https://doi.org/10.1016/j.bbapap.2005.08.017
  7. Karin, M.Z, G. Liu and E. Zandi. 1997. AP-1 function and egulation. Curr. Opin. Cell Biol. 9(2):240-246. https://doi.org/10.1016/S0955-0674(97)80068-3
  8. Kim, S.J., W.S. Chung, S.G. Kim and J.Y. Um. 2011. Antiinflammatory effect of Oldenlandia diffusa and its constituent, hentriacontane, through suppression of caspase-1 activation in mouse peritoneal macrophages. Phytother Res. 25(10): 1537-1546. https://doi.org/10.1002/ptr.3443
  9. Kim, S.J., H.J. Shin, B.J. Lee, D.S. Kim, J.H. Lee, M.Y. Jeong, H.L. Kim, J. Park, H. Lim, S.H. Kim, S.H. Hong, M.W. Hwang and J.Y. Um. 2014. The antiinflammatory mechanism of igongsan in mouse peritoneal macrophages via suppression of NF-kB/Caspase-1 activation. Phytother Res. 28(5):736-744. https://doi.org/10.1002/ptr.5058
  10. Limtrakul, P., S. Yodkeeree, P. Pitchakarn and W. Punfa. 2015. Suppression of inflammatory responses by black rice extract in RAW 264.7 macrophage cells via downregulation of NF-kB and AP-1 signaling pathways. Asian Pac J Cancer Prev. 16(10):4277-4283. https://doi.org/10.7314/APJCP.2015.16.10.4277
  11. Li, Q. and I.M. Verma.2002. NF-kappaB regulation in the immune system. Nat. Rev. Immunol. 2(10):725-734. https://doi.org/10.1038/nri910
  12. MacMicking. J., Q.W. Xie and C. Nathan. 1997. Nitric Oxide and macrophage function. Annu. Rev. Immunol. 15:323-330. https://doi.org/10.1146/annurev.immunol.15.1.323
  13. Medzhitov, R. and C.A. Janeway Jr. 1997. Innate immunity: The virtues of a nonclonal system of recognition. Cell 91(3):295-298. https://doi.org/10.1016/S0092-8674(00)80412-2
  14. Namkoong, S., Y.J. Kim, T. Kim and E.H. Sohn. 2012. Study on the immunomodulatory effects of ellagic acid and their mechanisms related to toll-like receptor 4 in macrophages. Korean J. Plant Res. 25(5):561-567. https://doi.org/10.7732/kjpr.2012.25.5.561
  15. Park, Y.D., C.H. Ji, D.S. Choi, M.W. Byun and J.Y. Jeong. 2011. Biological evaluation of isoegomaketone isolated from Perilla frutescens and its synthetic derivatives as antiinflammatory agents. Arch. Pharm. Res. 34(8):1277-1282. https://doi.org/10.1007/s12272-011-0806-8
  16. Park, M.Y., H.J. Kwon and M.K. Sung. 2009. Evauation of aloin and aloe-emodin as anti-inflammatory agents in aloe by using murine macrophages. Biosci. Biotechnol. Biochem. 73(4):828-832. https://doi.org/10.1271/bbb.80714
  17. Qian, Z., Z. Wu, L. Huang, H. Qiu, L. Wang, L. Li, L. Yao, K. Kang, J. Qu, Y. Wu, J. Luo, J.J. Liu, Y. Yang, W. Yang and D. Gou. 2015. Mulberry fruit prevents LPS-induced NF-${\kappa}B$/pERK/MAPK signals in macrophages and suppresses acute colitis and colorectal tumorigenesis in mice. Sci Rep. 1-12.
  18. Reinhart, K. and W. Karzai. 2001. Anti-tumor necrosis factor therapy in sepsis: Update on clinical trials and lessons learned. Crit. Care. Med. 29 (Suppl. 7):S121-S125. https://doi.org/10.1097/00003246-200107001-00037
  19. Rincon, M., C.G. Irvin. 2012. Role of IL-6 in asthma and other inflammatory pulmonary diseases. Int. J. biol. Sci. 8(9): 1281-1290. https://doi.org/10.7150/ijbs.4874
  20. Tina, J., J. Liu, J. Zhang, Z. Hu and X. Chen. 2003. Fluorescence studies on the interactions of barbaloin with bovine serum albumin. Chem. Pharm. Bull. 51(5):589-582. https://doi.org/10.1248/cpb.51.579
  21. Yagi, A., S. Hegazy, A. Kabbash and E.A. Wahab. 2009. Possible hypoglycemic effect of Aloe vera L. high molecular weight fractions on type 2 diabetic patients. Saudi Pharm J. 17(3):209-215. https://doi.org/10.1016/j.jsps.2009.08.007