Journal of Digestive Cancer Research

  • 제5권2호
  • /
  • Pages.105-112
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  • 2017
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  • 2950-9394(pISSN)
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  • 2950-9505(eISSN)
대한소화기암연구학회 (Korean Society of Gastrointestinal Cancer Research)
권호 표지

Helicobacter pylori 감염 위상피세포에서 MicroRNA 발현 변화

MicroRNA Profile in the Helicobacter pylori-infected Gastric Epithelial Cells

  • 김창환 (가톨릭대학교 의과대학 부천성모병원 내과학교실) ;
  • 김성수 (가톨릭대학교 의과대학 의정부성모병원 내과학교실) ;
  • 김태호 (가톨릭대학교 의과대학 부천성모병원 내과학교실) ;
  • 정우철 (가톨릭대학교 의과대학 성빈센트병원 내과학교실) ;
  • 김재광 (가톨릭대학교 의과대학 부천성모병원 내과학교실)
  • Chang Whan Kim (Department of Internal Medicine, Bucheon St. Mary's Hospital, The Catholic University of Korea) ;
  • Sung Soo Kim (Department of Internal Medicine, Uijeongbu St. Mary's Hospital, The Catholic University of Korea) ;
  • Tae Ho Kim (Department of Internal Medicine, Bucheon St. Mary's Hospital, The Catholic University of Korea) ;
  • Woo Chul Chung (Department of Internal Medicine, St. Vincent's Hospital, The Catholic University of Korea) ;
  • Jae Kwang Kim (Department of Internal Medicine, Bucheon St. Mary's Hospital, The Catholic University of Korea)
  • 투고 : 2017.11.02
  • 심사 : 2017.12.03
  • 발행 : 2017.12.31
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초록

위암 발병에 관여하는 Helicobacter pylori는 위상피세포내에서 많은 miRNA의 변화를 유도하여 발암과정에 역할을 할 것으로 추정하고 있다. 현재까지 H. pylori 감염 시 상피세포에서 miRNA 변화에 대해 명확히 밝혀져 있지 않다. 본 연구의 목적은 H. pylori에 감염된 위상피세포에서 miRNA의 발현 변화를 관찰하고자 하였다. H. pylori에 6시간 동안 감염시킨 AGS 위상피세포주와 AGS 세포주에 3개월 이상 장기간 H. pylori를 감염시켜 얻은 세포주(HS3C)를 대상으로 하였다. 대상 세포주로 부터 miRNA만을 분리한 후, custom microarray를 이용하여 발현 변화를 관찰하였다. 또한 microarray에서 유의한 증감이 관찰된 목표 유전자를 선별하여 real-time PCR을 이용하여 정량적 변화를 확인하였다. miRNA microarray 분석 결과를 토대로 변화가 관찰된 12개의 miRNA를 선별하였다. Real-time PCR 검사로 miRNA의 변화를 검정한 결과, miR-21, miR-221, miR-222은 6시간 동안 감염시킨 AGS 위상피세포주와 HS3C 세포주 모두에서 증가되어 있었다. miR-99b, miR-200b, miR-203b, miR-373은 6시간 동안 감염시킨 AGS 위상피세포주와 HS3C 세포주 모두에서 감소되어 있었다. miR-23a, miR-23b, miR-125b, miR-141, miR-155는 H. pylori에 6시간 동안 감염된 AGS 위상피세포주에서 감소되었으나, HS3C에서는 증가되어 있었다. H. pylori 감염 위상피세포주에서 miR-21, miR-99b, miR-125b, miR-200b, miR-203b, miR-221, miR-222, miR-373의 발현 변화는 위암의 발생기전에 관여할 것으로 추정되며, 각각의 기능과 역할의 규명에 대해서는 후속 연구가 필요하다.

Background: The expression of miRNAs in response to Helicobacter pylori infection has not been well explored. The aims of this study were to evaluate the H. pylori associated miRNAs in the gastric epithelial cells. Methods: We investigated gastric epithelial cell-line (HS3C) exposed H. pylori over 3 months and AGS cell-line (AGS) exposed H. pylori for 6 hour. After the extraction of miRNA from these cell-lines, microarray and real time PCR were performed to confirm the alteration of expression. Results: All 12 miRNAs chosen for real-time PCR are based on the result of microarray and their potential functions related to H. pylori infection. miR-21, miR-221, miR-222 were upregulated in the H. pylori infected AGS cell for 6 hours and HS3C cells. miR-99b, miR-200b, miR-203b and miR-373 were downregulated in the H. pylori infected AGS cell for 6 hours and HS3C cells. miR-23a, miR-23b, miR-125b, miR-141 and miR-155 were upregulated in HS3C cell line but not in H. pylori infected AGS cell for 6 hours. Conclusion: miR-21, miR-99b, miR-125b, miR-200b, miR-203b, miR-221, miR-222, and miR-373 are supposed to be related with oncogenesis of H. pylori infection. Further studies are needed for the evaluation of the function of these confirmed miRNAs.

키워드

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