Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effect of pyroligneous acids on urease inhibition

요소분해 저해에 미치는 목초액의 영향 평가

  • Park, Hyun Jun (Soil research institute, Purumbio Co. Ltd.) ;
  • Park, Jin Hee (School of Crop Science and Agricultural Chemistry, Chungbuk National University)
  • Received : 2017.05.23
  • Accepted : 2017.06.11
  • Published : 2017.06.30
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Abstract

This study was conducted to investigate the effect of pyroligneous acids on urea hydrolysis for the purpose of inhibiting ammonia volatilization during urea fertilizer application. Different types of synthetic urease inhibitors have been searched and developed, but their use is limited due to varying inhibition effects on soil urease, and environmental problems. In this study, the effect of pyroligneous acids, a natural substance, on urea hydrolysis in soil was evaluated by analyzing inhibition of urease activity. Pyroligneous acids inhibited plant urease and microbial urease activity, as well as soil urease with various urease complex. In addition, pyroligneous acids exhibited non-competitive urease inhibition effect through urease kinetics and inhibited urea hydrolysis in the soil. This study showed that pyroligneous acids treatment with urea fertilizer decreases the loss of urea fertilizer, improves the efficiency of nitrogen application on plant and reduces the amount of nitrogen fertilizers applied in soil.

본 연구는 요소비료를 처리할 때 가수분해가 일어나 암모니아로 휘산되어 손실되는 것을 억제하기 위한 목적으로 목초액이 요소분해에 미치는 영향을 규명하고자 하였다. 기존에 수많은 요소분해 합성억제제들이 개발 또는 탐색되어 왔으나, 토양 또는 환경 별로 효과가 일정치 않고 합성물질의 경우 환경에 미치는 영향을 고려해야 한다는 점 등 때문에 그 사용이 제한되고 있는 실정이다. 본 연구에서는 친환경 농업자재인 목초액이 토양 중 요소분해에 미치는 영향을 요소분해효소(urease)활성 억제효과를 통해 평가하였다. 목초액은 식물 urease와 미생물 urease 활성저해 효과뿐만 아니라 다양한 urease complex가 존재하는 토양 urease에도 저해효과를 보였다. 이러한 요소분해효소의 저해는 jack bean urease 반응속도를 측정한 결과 non-competitive inhibition으로 판단된다. 또한 목초액을 요소와 함께 토양에 처리하였을 경우 토양 내 요소분해작용을 억제하였다. 이를 통해 목초액을 요소 비료와 같이 처리할 경우 식물에 공급되는 질소의 효율을 증진시킴과 동시에 토양에 공급되는 질소비료의 총량을 절감하여 친환경 농업에 도움이 될 것으로 판단된다.

Keywords

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