Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Antioxidative and Cellular Protective Effects of Dolwoe (Gynostemma pentaphyllum Makino) Extracts against Oxidative Stress

돌외(Gynostemma pentaphyllum Makino) 추출물의 산화적 스트레스에 대한 항산화 및 세포보호효과

  • Kim, Kyoung Mi (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center) ;
  • Kim, A-Rang (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center) ;
  • Kim, A-Young (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center) ;
  • Ha, Ji Hun (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center) ;
  • Xuan, Song Hua (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center) ;
  • Jeong, Yoon Ju (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center) ;
  • Park, Young Min (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center) ;
  • Jeong, Hyo Jin (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center) ;
  • Hong, In Gi (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center) ;
  • Park, Soo Nam (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center)
  • 김경미 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 김아랑 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 김아영 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 하지훈 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 현송화 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 정윤주 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 박영민 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 정효진 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 홍인기 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 박수남 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터)
  • Received : 2017.05.17
  • Accepted : 2017.06.15
  • Published : 2017.06.30
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Abstract

In this study, we investigated the total phenolic and flavonoid contents, component analysis, antioxidative activity and cellular protective effects against oxidative stress on human skin cells in 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from Gynostemma pentaphyllum (G. pentaphyllum) Makino. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activites ($FSC_{50}$) of the 50% ethanol extracts, ethyl acetate fraction and aglycone fraction were 246.8, 147.2, $128.9{\mu}g/mL$, respectively. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of the 50% ethanol extract, ethyl acetate fraction and aglycone fraction on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system using the luminol-dependent chemiluminescence assay were 37.15, 10.74, $7.19{\mu}g/mL$, respectively. We investigated the cellular protective activity and the results showed that treatment of aglycone fraction ($0.05-0.39{\mu}g/mL$) protect human skin cells in a concentration-dependent manner when the skin cell damages were induced by treating them with $H_2O_2$. These results suggest that extract/fractions of G. pentaphyllum Makino may be applicable as natural antioxidants in cosmetics.

Keywords

References

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