Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibitory Effect of a Sesquiterpene from Artemisia iwayomogi on Expression of Inducible Nitric Oxide Synthase by Suppression of I-κBα Degradation in LPS-stimulated RAW 264.7 Cells

  • Kim, Na Yeon (College of Pharmacy, Sookmyung Women's University) ;
  • Koh, Hye Jin (College of Pharmacy, Sookmyung Women's University) ;
  • Li, Hua (College of Pharmacy, Sookmyung Women's University) ;
  • Lee, Hwa Jin (Department of Natural Medicine Resources, Semyung University) ;
  • Ryu, Jae-Ha (College of Pharmacy, Sookmyung Women's University)
  • Received : 2016.12.29
  • Accepted : 2017.01.30
  • Published : 2017.06.30
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Abstract

A sesquiterpene was purified from Artemisia iwayomogi methanolic extract during the course of searching anti-inflammatory principle from medicinal plants. A sesquiterpene identified as armefolin inhibited the production of nitric oxide (NO) and attenuated inducible nitric oxide synthase (iNOS) protein level in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Armefolin also down-regulated mRNA expressions of iNOS and pro-inflammatory cytokines, interleukin-$1{\beta}$ and interleukin-6 in LPS-activated macrophages. Moreover, armefolin suppressed the degradation of inhibitory-${\kappa}B{\alpha}$ (I-${\kappa}B{\alpha}$) in LPS-activated macrophages. These data suggest that armefolin from A. iwayomogi can suppress the LPS-induced production of NO and the expression of iNOS gene through inhibiting the degradation of I-${\kappa}B{\alpha}$. Taken together, armefolin from A. iwayomogi might be a candidate as promising anti-inflammatory agent.

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