Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Identification, Enzyme Assay, and Metabolic Profiling of Trichoderma spp.

  • Bae, Soo-Jung (Department of Biotechnology, Yeungnam University) ;
  • Park, Young-Hwan (Department of Biotechnology, Yeungnam University) ;
  • Bae, Hyeun-Jong (Department of Bioenergy Science and Technology, Chonnam National University) ;
  • Jeon, Junhyun (Department of Biotechnology, Yeungnam University) ;
  • Bae, Hanhong (Department of Biotechnology, Yeungnam University)
  • Received : 2017.02.24
  • Accepted : 2017.04.03
  • Published : 2017.06.28
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Abstract

The goal of this study was to identify and characterize selected Trichoderma isolates by metabolic profiling and enzyme assay for evaluation of their potential as biocontrol agents against plant pathogens. Trichoderma isolates were obtained from the Rural Development Administration Genebank Information Center (Wanju, Republic of Korea). Eleven Trichoderma isolates were re-identified using ribosomal DNA internal transcribed spacer (ITS) regions. ITS sequence results showed new identification of Trichoderma isolates. In addition, metabolic profiling of the ethyl acetate extracts of the liquid cultures of five Trichoderma isolates that showed the best anti-Phytophthora activities was conducted using gas chromatography-mass spectrometry. Metabolic profiling revealed that Trichoderma isolates shared common metabolites with well-known antifungal activities. Enzyme assays indicated strong cell wall-degrading enzyme activities of Trichoderma isolates. Overall, our results indicated that the selected Trichoderma isolates have great potential for use as biocontrol agents against plant pathogens.

Keywords

References

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