Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Burkholderia tropica as a Potential Microalgal Growth-Promoting Bacterium in the Biosorption of Mercury from Aqueous Solutions

  • Zarate, Ana (Doctoral Program in Applied Sciences - Coastal Marine Systems, Faculty of Marine Sciences and Biological Resources, University of Antofagasta) ;
  • Florez, July (Microalgae Biotechnology Research Group, Faculty of Basic Sciences, University of Atlantico) ;
  • Angulo, Edgardo (Microalgae Biotechnology Research Group, Faculty of Basic Sciences, University of Atlantico) ;
  • Varela-Prieto, Lourdes (Immunology and Molecular Biology Research Group, University of Atlantico) ;
  • Infante, Cherlys (Faculty of Pharmaceutical Sciences, Campus of Zaragocilla, University of Cartagena) ;
  • Barrios, Fredy (Faculty of Pharmaceutical Sciences, Campus of Zaragocilla, University of Cartagena) ;
  • Barraza, Beatriz (Faculty of Health Sciences, CIUL, Universuty Libre) ;
  • Gallardo, D.I (Department of Mathematics, Faculty of Engineering, University of Atacama) ;
  • Valdes, Jorge (Doctoral Program in Applied Sciences - Coastal Marine Systems, Faculty of Marine Sciences and Biological Resources, University of Antofagasta)
  • Received : 2016.11.21
  • Accepted : 2017.03.16
  • Published : 2017.06.28
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Abstract

The use of microalgal biomass is an interesting technology for the removal of heavy metals from aqueous solutions owing to its high metal-binding capacity, but the interactions with bacteria as a strategy for the removal of toxic metals have been poorly studied. The goal of the current research was to investigate the potential of Burkholderia tropica co-immobilized with Chlorella sp. in polyurethane discs for the biosorption of Hg(II) from aqueous solutions and to evaluate the influence of different Hg(II) concentrations (0.041, 1.0, and 10 mg/l) and their exposure to different contact times corresponding to intervals of 1, 2, 4, 8, 16, and 32 h. As expected, microalgal bacterial biomass adhered and grew to form a biofilm on the support. The biosorption data followed pseudo-second-order kinetics, and the adsorption equilibrium was well described by either Langmuir or Freundlich adsorption isotherm, reaching equilibrium from 1 h. In both bacterial and microalgal immobilization systems in the co-immobilization of Chlorella sp. and B. tropica to different concentrations of Hg(II), the kinetics of biosorption of Hg(II) was significantly higher before 60 min of contact time. The highest percentage of biosorption of Hg(II) achieved in the co-immobilization system was 95% at pH 6.4, at 3.6 g of biosorbent, $30{\pm}1^{\circ}C$, and a mercury concentration of 1 mg/l before 60 min of contact time. This study showed that co-immobilization with B. tropica has synergistic effects on biosorption of Hg(II) ions and merits consideration in the design of future strategies for the removal of toxic metals.

Keywords

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