Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enterococcus faecium FC-K Derived from Kimchi Is a Probiotic Strain That Shows Anti-Allergic Activity

  • Rho, Man-Kwang (Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Kim, Young-Eun (Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Rho, Hyun-In (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Tae-Rahk (Cellbion Co., Ltd) ;
  • Kim, Yoon-Bum (Department of Oriental Dermatology, College of Korean Medicine, Kyung Hee University) ;
  • Sung, Won-Kyung (Department of Oriental Dermatology, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Tae-Woo (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Dae-Ok (Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Kang, Hee (Graduate School of East-West Medical Science, Kyung Hee University)
  • Received : 2016.11.07
  • Accepted : 2017.03.15
  • Published : 2017.06.28
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Abstract

A rise in the occurrence of allergic diseases is attributed to the dysregulated balance of type 1/type 2 immunity, where type 2 T-helper (Th2) cells predominate over type 1 T-helper (Th1) cells, leading to an abnormally increased production of IgE in response to unharmful antigens. Kimchi, a traditional Korean fermented food, is a rich source of beneficial lactic acid bacteria. In this study, we investigated the ability of Enterococcus faecium FC-K derived from kimchi to induce type I immunity in the presence of Th2 polarizing conditions in vitro and in vivo. Stimulation of mouse peritoneal macrophages with E. faecium FC-K induced the production of tumor necrosis factor alpha, interleukin (IL)-6, and IL-12. Under the in vitro Th2 conditions in which splenic T cells were activated in the presence of IL-4, E. faecium FC-K enhanced the ability of T cells to produce interferon $(IFN)-{\gamma}$. Using the ovalbumin (OVA)-induced allergy model, male BALB/c mice receiving E. faecium FC-K reduced the serum level of total IgE, but not that of OVA-specific IgE. Furthermore, the population of activated splenic B cells during OVA immunization was decreased in E. faecium FC-K-treated mice, accounting for a reduction of total IgE in the serum. Restimulating splenocytes from OVA-immunized mice with OVA ex vivo resulted in an increased production of $IFN-{\gamma}$, with no effect on IL-4, in E. faecium FC-K-treated mice. These observations provide the evidence that E. faecium FC-K can be a beneficial probiotic strain that can modulate the Th2-mediated pathologic response.

Keywords

References

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