Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지
DOI QR코드

DOI QR Code

The Activity of Apo-transferrin on the Growth of Staphylococcus pseudintermedius

  • Bae, Seul-gi (Laboratory of Veterinary Dermatology, College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Youn-Ju (Laboratory of Veterinary Dermatology, College of Veterinary Medicine, Kyungpook National University) ;
  • Oh, Tae-Ho (Laboratory of Veterinary Dermatology, College of Veterinary Medicine, Kyungpook National University)
  • Received : 2017.03.06
  • Accepted : 2017.04.10
  • Published : 2017.04.28
Download PDF
⟨ Previous Next ⟩

Abstract

Apo-transferrin is an iron-binding protein that has been reported to have an antimicrobial effect. It is considered a major component of the host defense mechanism as it limits microbial access to iron. This study was performed to investigate whether bovine apo-transferrin would have an inhibitory effect on the growth of S. pseudintermedius, which is one of the most isolated bacteria from dogs, and to compare the antimicrobial efficacy with bovine holo-transferrin. S. pseudintermedius were grown at $37^{\circ}C$ in 96-well culture plates using Muller Hinton broth containing bovine apo-transferrin or bovine holo-transferrin at concentrations ranging from 0.5 or 2.5 to 5.0 mg/ml. The optical densities of the wells were then measured at 570 nm. In this study, the apo-transferrin showed dose-dependent antimicrobial effect against S. pseudintermedius while holo-transferrin did not inhibit the growth of S. pseudintermedius effectively. The results suggest that iron deprivation is an important pathway for inhibiting bacterial growth and bovine apo-transferrin has great antimicrobial effects against S. pseudintermedius.

Keywords

References

  1. Aguila A, Herrera AG, Morrison D, Cosgrove B, Perojo A, Montesinos I, Perez J, Sierra G, Gemmell CG, Brock JH. Bacteriostatic activity of human lactoferrin against Staphylococcus aureus is a function of its iron-binding properties and is not influenced by antibiotic resistance. FEMS Immunol Med Microbiol 2001; 31: 145-152. https://doi.org/10.1111/j.1574-695X.2001.tb00511.x
  2. Ahluwalia M, Brummer E, Sridhar S, Singh R, Stevens D. Isolation and characterisation of an anticryptococcal protein in human cerebrospinal fluid. J Med Microbiol 2001; 50: 83-89. https://doi.org/10.1099/0022-1317-50-1-83
  3. Ardehali R, Shi L, Janatova J, Mohammad SF, Burns GL. The Effect of Apo Transferrin on Bacterial Adhesion to Biomaterials. Artif Organs 2002; 26: 512-520. https://doi.org/10.1046/j.1525-1594.2002.06923.x
  4. Ardehali R, Shi L, Janatova J, Mohammad SF, Burns GL. The inhibitory activity of serum to prevent bacterial adhesion is mainly due to apo transferrin. Biomed Mater Res A 2003; 66: 21-28.
  5. Bannoehr J, Guardabassi L. Staphylococcus pseudintermedius in the dog: taxonomy, diagnostics, ecology, epidemiology and pathogenicity. Vet Dermatol 2012; 23: 253-e252. https://doi.org/10.1111/j.1365-3164.2012.01046.x
  6. Beutler E, Gelbart T, Lee P, Trevino R, Fernandez MA, Fairbanks VF. Molecular characterization of a case of atransferrinemia. Blood 2000; 96: 4071-4074.
  7. Boelaert JR, Vandecasteele SJ, Appelberg R, Gordeuk VR. The effect of the host's iron status on tuberculosis. J Infect Dis 2007; 195: 1745-1753. https://doi.org/10.1086/518040
  8. Bond R, Kim J, Lloyd D. Bovine and canine transferrin inhibit the growth of Malassezia pachydermatis in vitro. Med Mycol 2005; 43: 447-451. https://doi.org/10.1080/13693780400020154
  9. Bullen J, Leigh L, Rogers HJ. The effect of iron compounds on the virulence of Escherichia coli for guinea-pigs. Immunology 1968; 15: 581-588.
  10. Bullen JJ, Rogers HJ, Spalding PB, Ward CG. Iron and infection: the heart of the matter. FEMS Immunol Medl Microbiol 2005; 43: 325-330. https://doi.org/10.1016/j.femsim.2004.11.010
  11. Ellison RD, Giehl TJ, LaForce FM. Damage of the outer membrane of enteric gram-negative bacteria by lactoferrin and transferrin. Infect Immun 1988; 56: 2774-2781.
  12. Ganz T. Iron in innate immunity: starve the invaders. Curr Opin Immunol 2009; 21: 63-67. https://doi.org/10.1016/j.coi.2009.01.011
  13. Geoghegan JA, Smith EJ, Speziale P, Foster TJ. Staphylococcus pseudintermedius expresses surface proteins that closely resemble those from Staphylococcus aureus. Vet Microbiol 2009; 138: 345-352. https://doi.org/10.1016/j.vetmic.2009.03.030
  14. Gray-Owen SD, Schyvers AB. Bacterial transferrin and lactoferrin receptors. Trends Microbiol 1996; 4: 185-191. https://doi.org/10.1016/0966-842X(96)10025-1
  15. Hirose M. The structural mechanism for iron uptake and release by transferrins. Biosci Biotechnol Biochem 2000; 64: 1328-1336. https://doi.org/10.1271/bbb.64.1328
  16. Holbein BE. Enhancement of Neisseria meningitidis infection in mice by addition of iron bound to transferrin. Infect Immun 1981; 34: 120-125.
  17. Kansal RG, Aziz RK, Kotb M. Modulation of expression of superantigens by human transferrin and lactoferrin: a novel mechanism in host-Streptococcus interactions. J Infect Dis 2005; 191: 2121-2129. https://doi.org/10.1086/430386
  18. Khan FA, Fisher MA, Khakoo RA. Association of hemochromatosis with infectious diseases: expanding spectrum. Int J Infect Dis 2007; 11: 482-487. https://doi.org/10.1016/j.ijid.2007.04.007
  19. Matinaho S, von Bonsdorff L, Rouhiainen A, Lonnroth M, Parkkinen J. Dependence of Staphylococcus epidermidis on non-transferrin-bound iron for growth. FEMS Microbiol Lett 2001; 196: 177-182. https://doi.org/10.1111/j.1574-6968.2001.tb10561.x
  20. McEwan N. Adherence by Staphylococcus intermedius to canine keratinocytes in atopic dermatitis. Res Vet Sci 2000; 68: 279-283. https://doi.org/10.1053/rvsc.2000.0378
  21. Miller WH, Griffin CE, Campbell KL, Muller GH. Muller and Kirk's Small Animal Dermatology, 7th ed. St. Louis: Elsevier Health Sciences; 2013: 184-187
  22. Modun BJ, Cockayne A, Finch R, Williams P. The Staphylococcus aureus and Staphylococcus epidermidis transferrinbinding proteins are expressed in vivo during infection. Microbiology 1998; 144: 1005-1012. https://doi.org/10.1099/00221287-144-4-1005
  23. Morris DO, Rook KA, Shofer FS, Rankin SC. Screening of Staphylococcus aureus, Staphylococcus intermedius, and Staphylococcus schleiferi isolates obtained from small companion animals for antimicrobial resistance: a retrospective review of 749 isolates (2003-04). Vet Dermatol 2006; 17: 332-337. https://doi.org/10.1111/j.1365-3164.2006.00536.x
  24. Park RY, Sun HY, Choi MH, Bai YH, Shin SH. Staphylococcus aureus siderophore-mediated iron-acquisition system plays a dominant and essential role in the utilization of transferrin-bound iron. J Microbiol 2005; 43: 183-190.
  25. von Bonsdorff L, Sahlstedt L, Ebeling F, Ruutu T, Parkkinen J. Apotransferrin administration prevents growth of Staphylococcus epidermidis in serum of stem cell transplant patients by binding of free iron. FEMS Immunol Med Microbiol 2003; 37: 45-51. https://doi.org/10.1016/S0928-8244(03)00109-3