Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Fucoidan Suppresses Prostaglandin E2 Production and Akt Activation in Lipopolysaccharide-Stimulated Porcine Peripheral Blood Mononuclear Cells

  • Park, Geon-Tae (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University) ;
  • Ahn, Changhwan (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Byeong-Teck (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Ji-Houn (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University) ;
  • Jeung, Eui-Bae (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University) ;
  • Yang, Mhan-Pyo (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University)
  • Received : 2017.03.17
  • Accepted : 2017.06.13
  • Published : 2017.06.30
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Abstract

Fucoidan, a cell wall polysaccharide found in the brown seaweed, is reported to have broad-spectrum biological activities. The objectives of this study were to examine the effect of fucoidan on prostaglandin $E_2$ ($PGE_2$) and cyclooxygenase-2 (COX-2) expression in lipopolysaccharide (LPS)-stimulated porcine peripheral blood mononuclear cells (PBMCs) and to determine whether these effects are involved in Akt activation. The levels of $PGE_2$ production in the culture supernatants from PBMCs were determined by the enzyme-linked immunosorbent assay (ELISA) kit and the levels of COX-2 mRNA were measured by real time polymerase chain reaction (RT-PCR). Akt activity was determined by Western blot analysis. Fucoidan in LPS-$na{\ddot{i}ve}$ PBMCs has no effect on $PGE_2$ production and COX-2 mRNA expression. Furthermore, fucoidan does not affect Akt activation in LPS- $na{\ddot{i}ve}$ PBMCs. However, $PGE_2$ production and COX-2 mRNA expression on PBMCs were remarkably enhanced by LPS stimulation. Akt activity was also increased by LPS. Increasing effects of $PGE_2$ production and COX-2 mRNA expression in PBMCs induced by LPS were suppressed by addition of fucoidan. In addition, fucoidan reduced an increase in Akt activity in LPS-stimulated PBMCs. These results suggested that fucoidan exerts potent anti-inflammatory properties by suppression of $PGE_2$ production, COX-2 mRNA expression and Akt activation in LPS-stimulated PBMCs.

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