Journal of Magnetics

The Korean Magnetics Society (한국자기학회)
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Development of a Magnetic-field Stimulation System for Cell Cultures in situ: Simulation by Finite Element Analysis

  • Dominguez, G. (The Electrical Engineering Department, Center for Research and Advanced Studies) ;
  • Arias, S. (Electronics Department, Autonomous Metropolitan University) ;
  • Reyes, Jose L. (Physiology, Biophysics and Neuroscience Department, Center for Research and Advanced Studies) ;
  • Rogeli, Pablo (The Electrical Engineering Department, Center for Research and Advanced Studies)
  • Received : 2017.03.09
  • Accepted : 2017.06.07
  • Published : 2017.06.30
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Abstract

The effects of exposure to an extremely low-frequency magnetic field (25 Hz 20G) on animal cells have been studied. In some reports, stimulation was performed for fixed frequency and variations in magnitude; however, animal-cell experiments have established that both parameters play an important role. The present work undertook the modeling, simulation, and development of a uniform-magnetic-field generation system with variable frequency and stimulation intensity (0-60 Hz, 1-25G) for experimentation with cell cultures in situ. The results showed a coefficient of variation less than 1 % of the magnetic-field dispersion at the working volume, which is consistent with the corresponding simulation results demonstrating a uniform magnetic field. On the other hand, long-term tests during the characterization process indicated that increments of only $0.4^{\circ}C$ in the working volume temperature will not be an interfering factor when experiments are carried out in in situ cell cultures.

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References

  1. S. Zannella, Proceedings of CAS CERN Accelerator School. Anacapri, Italy (1997).
  2. W. R. Adey, Proc IEEE. 68, 1 (1980). https://doi.org/10.1109/PROC.1980.11693
  3. L. Potenza, L. Cucchiarini, E. Piatti, U. Angelini, and M. Dacha, Bioelectromagnetics 25, 5 (2004). https://doi.org/10.1002/bem.10154
  4. B. Tenuzzo, A. Chionna, E. Panzarini, R. Lanubile, P. Tarantino, B. Di Jeso, M. Dwikat, and L. Dini, Bioelectromagnetics 27, 7 (2006).
  5. L. Dini and L. Abbro. Micron 36, 3 (2005).
  6. S. Ahmadian, R. Z. Saeed, and B. Bahram, Iran. Biomed. J. 10, 1 (2006).
  7. E. Lindstrom, P. Lindstrom, A. Berglund, E. Lundgren, and K. Hansson, Bioelectromagnetics 16, 1 (1995).
  8. C. Fanelli, S. Coppola, R. Barone, C. Colussi, G. Gualandi, P. Volpe, and L. Ghibelli, FASEB J. 13, 1 (1999). https://doi.org/10.1096/fasebj.13.1.1
  9. Y. Ren, J. Zhang, X. Wang, L. Xie, and B. Liu, Metallurgical and Mining Industry 7 (2015).
  10. J. Darnell, H. Lodish, and D. Baltimore, Molecular cell biology, 2nd ed. Scientific American Books, New York (1990) pp. 235-240.
  11. W. B. Jakoby, and I. H. Pastan, Cell culture, Elsevier (1979) pp. 554-556.
  12. S. Magdaleno, J. C. Olivares, E. Campero, R. Escarela, and E. Blanco, Proceedings of the COMSOL Conference 13 (2010).
  13. J. Kirschvink, Bioelectromagnetics 13, 5 (1992).
  14. D. Cvetkovic and I. Cosic, IEEE EMBS (2007) pp. 1675-1678.
  15. K. Dokladny, W. Wharton, R. Lobb, T. Y. Ma, and P. L. Moseley, Cell Stress Chaperones 11, 3 (2006).