Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
권호 표지
DOI QR코드

DOI QR Code

Ginsenosides analysis in the crude saponin fraction extracted from Korean red ginseng, and its efficacious analysis against acute pulmonary inflammation in mice

  • Lee, Seung Min (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Lim, Heung Bin (Department of Industrial Plant Science & Technology, Chungbuk National University)
  • Received : 2017.03.20
  • Accepted : 2017.05.08
  • Published : 2017.06.25
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we isolated ginseng crude saponin (GCS) from Korean red ginseng (KRG) and determined the ginsenoside content in it to investigate the physiological and pathological effects of GCS on acute pulmonary inflammation induced by intratracheal instillation of cigarette smoke condensates (CSC) and lipopolysaccharide (LPS) solution in BALB/c mice. GCS was orally administered at doses of 10 mg/kg and 25 mg/kg for 3 weeks. The recovery rate of GCS from KRG was 6.5 % and total ginsenosides from GCS was 1.13 %, and the content of Rb1 was the highest among them. Total inflammatory cells in the lung homogenates and bronchoalveolar lavage fluid (BALF) increased following intratracheal administration of CSC and LPS. However, GCS administration impaired this increase. Furthermore, it inhibited the increase in leukocytes in the blood, considerably decreased neutrophils in BALF, and declined infiltration of inflammatory cells and deposition of collagen in the tracheal and alveolar tissue. In this study, GCS was found to have a protective effect against acute pulmonary inflammation and it may be beneficial in preventing various respiratory diseases.

Keywords

References

  1. T. K. Yun, J. Kor. Med. Sci., 16(Suppl), S3 (2001). https://doi.org/10.3346/jkms.2001.16.S.S3
  2. M. Hong, Y. H. Lee, S. Kim, C. S. Bang, J. H. Yoon, G. H. Baik, D. J. Kim, and M. J. Kim. J. Ginseng Res., 40(3), 203-210 (2016). https://doi.org/10.1016/j.jgr.2015.07.006
  3. S. I. Chung, M. Y. Kang, and S. C. Lee, Prev. Nutr. Food Sci., 21(1), 24-30 (2016). https://doi.org/10.3746/pnf.2016.21.1.24
  4. J. G. Park, Y. J. Son, A. Aravinthan, J. H. Kim, and J. Y. Cho, J. Ginseng Res., 40(4), 431-436 (2016). https://doi.org/10.1016/j.jgr.2016.07.006
  5. H. U. Lee, E. A. Bae, M. J. Han, N. J. Kim, and D. H. Kim, Liver Int., 25(5), 1069-1073 (2005). https://doi.org/10.1111/j.1478-3231.2005.01068.x
  6. D. H. Kim, J. H. Chung, J. S. Yoon, Y. M. Ha, S. Bae, E. K. Lee, and H. Y. Chung, J. Ginseng Res., 37(1), 54-60 (2013). https://doi.org/10.5142/jgr.2013.37.54
  7. J. H. Kim and J. S. Choi, Physiol. Res., 65(6), 1031-1037 (2016).
  8. J. Bousquet, J. Kiley, E. D. Bateman, G. Viegi, A. A. Cruz, N. Khaltaev, and G. W. Canonica, Eur. Respir. J., 36(5), 995-1001 (2010). https://doi.org/10.1183/09031936.00012610
  9. H. Oh and Y. E Lee, Osong Public. Health Res. Perspect., 7(6), 385-393 (2016). https://doi.org/10.1016/j.phrp.2016.11.006
  10. J. Lee. J. H. Lee, J. A. Kim, and C. K. Rhee, Int. J. Chron. Obstruct Pulmon Dis., 12, 27-33 (2016). https://doi.org/10.2147/COPD.S121687
  11. J. Vestbo, S. S. Hurd, A. G. Aqusti, P. W. Jones, C. Vogelmeier, A. Anzueto, P. J. Barnes, L. M. Fabbri, F. J. Martinez, M. Nishimura, R. A. Stockley, D. D. Sin, and R. Rodriquez-Roisin, Am. J. Respir Crit. Care Med., 187(4), 347-365 (2013). https://doi.org/10.1164/rccm.201204-0596PP
  12. M. Maskey-Warzechowska, P. Neiman-Gryz, K. Osinka, P. Lis, K. Malesa, K. Gorska, and R. Krenke, Adv. Exp. Med. Biol., 944, 73-78 (2017).
  13. M. Kaur and D. Singh, J. Pharmacol. Exp. Ther., 347(11), 173-180 (2013). https://doi.org/10.1124/jpet.112.201855
  14. K. H. Lee, C. H. Lee, J. Jeong, A. H. Jang, and C. G. Yoo, J. Biol. Chem., 290(47), 28438-28445 (2015). https://doi.org/10.1074/jbc.M115.663567
  15. A. Bhowmik, T. A. Seemungal, R. J. Sapaford, and J. A. Wedzicha, JA, Thorax., 55(2), 114-120 (2000). https://doi.org/10.1136/thorax.55.2.114
  16. J. C. Hogg, F. Chu, S. Utokaparch, R. Woods, W. . Elliott, L. Buzatu, and P. D. Pare, New. Eng. J. Med., 350(26), 2645-2653 (2004). https://doi.org/10.1056/NEJMoa032158
  17. D. Raghavan, T. Bartter, and M. Joshi, Curr. Opin. Pulm. Med., 22(2), 106-112 (2016). https://doi.org/10.1097/MCP.0000000000000245
  18. O. Kornmann, R. Dahl, S. Centanni, A. Dogra, R. Owen, C. Lassen, and B. Kramer, Eur. Respir. J., 37(2), 273-279 (2011). https://doi.org/10.1183/09031936.00045810
  19. S. Apikoglo-Rabus, G. Yesilyaprak, and F. V. Izzettin, Respir. Med., 120, 109-115 (2016). https://doi.org/10.1016/j.rmed.2016.10.006
  20. H. W. Ryu, S. U. Lee, S. Lee, H. H. Song, T. H. Son, Y. U. Kim, H. J. Yuk, H. Ro, C. K. Lee, S. T. Hong, and S. R. Oh, Cytokine, 91, 57-64 (2016).
  21. H. G. Kwak and H. B. Lim, Chin. J. Nat. Med., 12(9), 641-647 (2014). https://doi.org/10.1016/S1875-5364(14)60098-4
  22. S. K. Kim, Y. S. Kwak, S. W. Kim, S. Y. Hwang, Y. S. Ko, and C. M. You, J. Ginseng Res., 22(3), 155-160 (1998).
  23. S. Shibata, O. Tanaka, T. Ando, M. Sado, S. Tsushima, and T. Ohsawa, Chem. Pharm. Bull.(Tokyo), 14(6), 595-600 (1966). https://doi.org/10.1248/cpb.14.595
  24. X. Wang, T. Sakuma, E. Asafu-adjaye, and G. K. Shiu, Anal. Chem., 71(8), 1579-1584 (1999). https://doi.org/10.1021/ac980890p
  25. Y. Chen, Z. Zhao, H. Chen, E. Brand, T. Yi, M. Qin, and Z. Liang, Int. Immunopharmacol., 28(2), 1097-1101 (2017). https://doi.org/10.1016/j.intimp.2015.07.041
  26. H. Lee, K. H. Jung, H. Lee, S. Park, W. Choi, and H. Bae, Int. Immunopharmacol., 28(2), 1097-1101 (2015). https://doi.org/10.1016/j.intimp.2015.07.041
  27. K. Kuwano, J. Araya, H. Hara, S. Minagawa, N. Takasaka, S. Ito, K. Kobayashi, and K. Nakayama, Respir. Investig., 54(6), 397-406 (2016). https://doi.org/10.1016/j.resinv.2016.03.010
  28. L. Cooling, S. Hoffmann, D. Webb, M. Meade, C. Yamada, R. Davenport, and G. Yanik, J. Clin. Apheresis, 32(1), 35-48 (2017). https://doi.org/10.1002/jca.21465
  29. D. D. Sin and S. P. Man, Circulation, 107(11), 1514-1519 (2003). https://doi.org/10.1161/01.CIR.0000056767.69054.B3
  30. N. Takabatake, H. Nakamura, S. Abe, S. Inoue, T. Hino, H. Saito, and H. Tomoike, Am. J. Respir. Crit. Care Med., 161(4), 1179-1184 (2000). https://doi.org/10.1164/ajrccm.161.4.9903022
  31. I. S. Shin, K. S. Ahn, N. R. Shin, H. J. Lee, H. W. Ryu, J. W. Kim, K. Y. Sohn, H. J. Kim, Y. H. Han, and S. R. Oh, Int. Immunopharmacol., 30, 62-68 (2016). https://doi.org/10.1016/j.intimp.2015.11.025
  32. P. J. Barnes, S. D. Shapiro, and R. A. Pauwels, Europ. Respir. J., 22(4), 672-688 (2003). https://doi.org/10.1183/09031936.03.00040703
  33. J. L. Wright and A. Churg, Histopathology, 49(1), 1-9 (2006). https://doi.org/10.1111/j.1365-2559.2006.02395.x
  34. T. Min, M. Bodas, S. Mazur, and N. Vij, J. Molec. Med., 86(6), 577-593 (2011).