Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Anti-inflammatory effects of ethanolic mulberry extract on the murine macrophage cell line, RAW 264.7

RAW 264.7 큰포식세포에서 상백피 및 상지 에탄올 추출물의 항염증 활성 비교

  • Kim, Yunyoung (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Yang, Yoon Kyoung (Department of Nutritional Science and Food Management, Soongeui Women's College) ;
  • Kim, Dongmin (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Kim, Ji Yeon (Department of Food Science and Technology, Seoul National University of Science and Technology)
  • 김윤영 (서울과학기술대학교 식품공학과) ;
  • 양윤경 (숭의여자대학교 식품영양학과) ;
  • 김동민 (서울과학기술대학교 식품공학과) ;
  • 김지연 (서울과학기술대학교 식품공학과)
  • Received : 2017.01.02
  • Accepted : 2017.03.05
  • Published : 2017.06.30
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Abstract

The aim of this study was to compare the anti-inflammatory effects of ethanol extracts of root peel and spear of mulberry (RME and SME, respectively) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Both extracts significantly inhibited the production of reactive oxygen species (ROS), nitric oxide (NO), and interleukin-6 (IL-6). However, prostaglandin $E_2$ ($PGE_2$) levels decreased in LPS-stimulated RAW 264.7 cells treated with SME. Additionally, the extracts reduced inducible NO synthase (iNOS) expression and cyclooxygenase-2 (COX-2) in mRNA levels. Although ROS production was lower in the RME-treated cells than in the SME-treated cells, the levels of other inflammatory parameters, including IL-6 and $PGE_2$, and mRNA levels of iNOS and COX-2 reduced more in the SME-treated cells. These results indicate that SME showed higher anti-inflammatory activities than RME. Therefore, SME can be used as a functional food ingredient to enhance health.

상백피 및 상지 에탄올 추출물의 항염증 효과를 확인하기 위하여 RAW 264.7 세포를 사용하여 시험하였다. 상백피, 상지 추출물의 활성산소종, 산화질소(II), 프로스타글란딘 $E_2$, 인터류킨-6, 종양괴사인자-${\alpha}$ 등의 생성능과 COX-2, iNOS mRNA 발현을 확인하였다. 산화질소(II) 생성량은 두 추출물 모두 유의하게 비슷한 정도로 감소시켰으나, 활성산소종 생성에서는 상백피 추출물이 상지 추출물 보다 억제 효과가 높게 나타났다. 프로스타글란딘 $E_2$와 인터류킨-6 생성에서는 상지 추출물의 억제 효과가 강하게 나타났으며, COX-2와 iNOS mRNA 발현량 또한 상지 추출물의 상백피에 비해 유의하게 감소시킴을 확인하였다. 본 연구에서 확인한 결과로 상백피, 상지 추출물 모두 염증 매개인자 발현 억제를 통해 항염증 효과를 가짐을 알 수 있었으며, 상지 추출물이 상백피 추출물 보다 염증 완화 효과가 강한 것으로 나타났다. 향후에는 상백피 및 상지 추출물의 주요 활성 성분들의 차이와, 염증완화 메커니즘을 통한 관련 질환에 효과가 있는지 정확한 작용메커니즘 확인 등의 분석실험이 필요할 것으로 생각된다.

Keywords

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