Journal of Korean Neurosurgical Society

  • 제60권4호
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  • Pages.391-396
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  • 2017
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  • 2005-3711(pISSN)
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  • 1598-7876(eISSN)
대한신경외과학회 (The Korean Neurosurgical Society)
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Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke

  • Wei, Hu (Department of Neurology, Affiliated Provincial Hospital of Anhui Medical University) ;
  • Sun, Tao (Department of Neurology, Nanjing General Hospital of Nanjing Command) ;
  • Tian, Yanghua (Department of Neurology, the First Hospital of Anhui Medical University) ;
  • Wang, Kai (Department of Neurology, the First Hospital of Anhui Medical University)
  • 투고 : 2016.10.25
  • 심사 : 2017.02.23
  • 발행 : 2017.07.01
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초록

Objective : To investigate the neuroprotective effects of Ginkgolide B (GB) against ischemic stroke-induced injury in vivo and in vitro, and further explore the possible mechanisms concerned. Methods : Transient middle cerebral artery occlusion (tMCAO) mice and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated N2a cells were used to explore the neuroprotective effects of GB. The expression of brain-derived neurotrophic factor (BDNF) was detected via Western blot and qRT-PCR. Results : GB treatment (4 mg/kg, i. p., bid) significantly reduced neurological deficits, water content, and cerebral infarct volume in tMCAO mice. GB also significantly increased Bcl-2/Bax ratio, reduced the expression of caspase-3, and protected against OGD/R-induced neuronal apoptosis. Meanwhile, GB caused the up-regulation of BDNF protein in vivo and in vitro. Conclusion : Our data suggest that GB might protect the brain against ischemic insult partly via modulating BDNF expression.

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참고문헌

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