한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제32권3호
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  • Pages.222-227
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  • 2017
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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대기압 유전체장벽방전 플라즈마에 의한 식품유해 미생물 살균

Sterilization of Food-Borne Pathogenic Bacteria by Atmospheric Pressure Dielectric Barrier Discharge Plasma

  • 이승제 (전라북도생물산업진흥원) ;
  • 송윤석 (전라북도생물산업진흥원) ;
  • 박유리 (전라북도생물산업진흥원) ;
  • 유승민 (국가핵융합연구소 플라즈마기술연구센터) ;
  • 전형원 (국가핵융합연구소 플라즈마기술연구센터) ;
  • 엄상흠 (국가핵융합연구소 플라즈마기술연구센터)
  • Lee, Seung Je (Research and Development Office, Jeonbuk Institute for Food-Bioindustry) ;
  • Song, Yoon Seok (Research and Development Office, Jeonbuk Institute for Food-Bioindustry) ;
  • Park, Yu Ri (Research and Development Office, Jeonbuk Institute for Food-Bioindustry) ;
  • Ryu, Seung Min (Innovation Technology Research Division, Plasma Technology Research Center, National Fusion Research Institute) ;
  • Jeon, Hyeong Won (Innovation Technology Research Division, Plasma Technology Research Center, National Fusion Research Institute) ;
  • Eom, Sang Heum (Innovation Technology Research Division, Plasma Technology Research Center, National Fusion Research Institute)
  • 투고 : 2016.11.21
  • 심사 : 2017.05.24
  • 발행 : 2017.06.30
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초록

연구는 대기압 유전체장벽방전 플라즈마 처리에 따른 식품유해 미생물 사멸효과를 조사하기 위해 수행되었다. 플라즈마 처리 시, 활성종 생성 및 농도에 영향을 미치는 노출시간, 노출거리, 산소비율, 전력 변화에 따른 E. coli의 사멸효과를 조사한 결과, E. coli의 사멸율은 플라즈마 처리를 위한 노출시간, 산소비율, 전력의 증가에 따라 증가한 반면, 노출거리의 증가에 따라서는 사멸율이 감소하였다. 이 결과는 미생물 시료가 플라즈마에 노출되는 시간이 증가됨으로서 시료 내 NO 농도가 증가되고, E. coli의 사멸율 역시 증가되는 결과로 뒷받침할 수 있고, 미생물 사멸효과를 높이기 위해서는 활성종의 농도가 증가되어야 함을 의미한다. E. coli와 함께 B. cereus, B. subtilis, B. thuringiensis, B. atrophaeus를 대상으로 대기압 유전체 장벽방전 플라즈마에 의한 살균효과를 조사한 결과, 72.3~91.3%의 높은 사멸율을 나타내었다. 이러한 결과로 미루어, 대기압 유전체장벽방전 플라즈마기술은 다양한 미생물에 적용될 수 있는 유용한 살균기술임을 확인하였다.

This study aimed to explore the potential for food-industry application of atmospheric pressure dielectric barrier discharge plasma (atmospheric pressure DBD plasma) as a non-thermal sterilization technology for microorganism. The effects of the key parameters such as power, oxygen ratio, exposure time and distance on Escherichia coli KCCM 21052 sterilization by the atmospheric pressure DBD plasma treatment were investigated. The experimental results revealed that increasing the power, exposure time or oxygen ratio and decreasing the exposure distance led to an improvement in the sterilization efficiency of E. coli. Furthermore, the atmospheric pressure DBD plasma (1.0 kW power, 1.0% (v/v) $O_2$, 5 min exposure time and 20 mm exposure distance) treatment was very effective for the sterilization of food-borne pathogenic bacteria. The sterilization rate of E. coli, Bacillus cereus KCCM 40935, Bacillus subtilis KCCM 12027, Bacillus thuringiensis KCCM 11429 and Bacillus atrophaeus KCCM 11314 were 72.3%, 74.6%, 88.5%, 84.7% and 91.3%, respectively.

키워드

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