한방비만학회지 (Journal of Korean Medicine for Obesity Research)

  • 제17권1호
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  • Pages.20-28
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  • 2017
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  • 1976-9334(pISSN)
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  • 2288-1522(eISSN)
한방비만학회 (The Society of Korean Medicine for obesity Research)
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고지방식이로 비만이 유도된 C57BL/6 마우스에서 Metformin과 황금추출물의 병용 투여 시 비만관련 지표에 미치는 영향

The Effect of Scutellariae Radix Combined with Metformin on Obesity-Relating Biomarker in High Fat Fed C57BL/6 Mice

  • 장세주 (동국대학교 한의과대학 한방재활의학과교실) ;
  • 왕경화 (동국대학교 한의과대학 한방재활의학과교실) ;
  • 최한석 (동국대학교 의과대학 내분비내과교실) ;
  • 진영원 (동국대학교 약학대학 약학교실) ;
  • 김호준 (동국대학교 한의과대학 한방재활의학과교실)
  • Chang, Seju (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University) ;
  • Wang, Jing-Hua (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University) ;
  • Choi, Han-Seok (Department of Endocrinology Medicine, College of Medicine, Dongguk University) ;
  • Chin, Young-Won (Department of Pharmacy, College of Pharmacy, Dongguk University) ;
  • Kim, Hojun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University)
  • 투고 : 2017.05.07
  • 심사 : 2017.06.12
  • 발행 : 2017.06.30
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초록

Objectives: The purpose of this study was to investigate anti-obesity effect of Scutellariae Radix extract combined with metformin. Methods: Male C57BL/6 mice, 4 weeks of age, were used to set high-fat diet induced obesity model. They were grouped NOR (normal control), HFD (high fat diet control), MET (metformin, 100 mg/kg/day), MH2 (metformin 50 mg/kg/day+Scutellariae Radix 200 mg/kg/day), and HG4 (Scutellariae Radix 400 mg/kg/day). MET, MH2, and HG4 were orally administered for 10 weeks. Body weight was measured every week. Fasting blood glucose, triglyceride, total cholesterol (TC), high density lipoprotein, glutamic oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) were measured before sacrifice. Oral glucose tolerance test (OGTT) was also performed. Organ weight and internal fat weight were measured after sacrifice. Results: MH2 group showed a reduction of body weight when compared with HFD group. MH2 group showed stable blood level control which was calculated areas under the curves by OGTT. TC, GOT, GPT level, internal fat, and organ weight in MH2 group reduced. Conclusions: The combined treatment of Scutellariae Radix and Metformin has impact on treating obesity. Further studies are needed to evaluate the effect of Metformin and herbal medicine combination therapy.

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참고문헌

  1. Haslan DW, James WP. Obesity. Lancet. 2005 ; 366(9442) : 1197-209. https://doi.org/10.1016/S0140-6736(05)67483-1
  2. Dandona P, Aljada A, Chaudhuri A, Mohanty P, Garg R. Metabolic syndrome a comprehensive perspective based on interation between obesity, diabetes, and inflammation. Circulation. 2005 ; 111(11) : 1148-54. https://doi.org/10.1161/01.CIR.0000157160.69812.55
  3. Carriere A, Carmona MC, Fernandez Y. Mitochondrial reactive oxygen species control thetranscription factor CHOP-10/ GADD153 andadipocyte differentiation: a mechanism forhypoxia-dependent effect. J Biol Chem. 2004 ; 279(39) : 40462-9. https://doi.org/10.1074/jbc.M407258200
  4. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007 ; 39(1) :44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  5. Kim HJ, Lee SG, Park SJ, Yu MH, Lee EJ, Lee SP, et al. Antioxidant effects of extracts from fermented red ginseng added with medicinal herbs in STZ-induced diabetic rats. Korean J Food Sci Thchnol. 2012 ; 44(3) : 367-72. https://doi.org/10.9721/KJFST.2012.44.3.367
  6. Kim KS, Kim LH, Rhee YJ, Lee SH, Choi JH, Ko HN. Analysis on research trend of studies related with Scutellariae Radix in Korea. Korean J Orient Physiol Pathol. 2011 ; 25(6) : 1095-101.
  7. Kim EH, Shim B, Kang S, Jeong G, Lee JS, Yu YB, et al. Anti-inflammatory effects of Scutellaria baicalensis extract via suppression of immune modulators and MAP kinase signaling molecules. J Ethnopharmacol. 2009 ; 126(2) : 320-31. https://doi.org/10.1016/j.jep.2009.08.027
  8. Song K, Lee S, Kim B, Park A, Kim A. Extracts of Scutellaria baicalensis reduced body weight and blood triglyceride in db/db mice. Phytother Res. 2013 ; 27(2) : 244-50. https://doi.org/10.1002/ptr.4691
  9. Yoon HJ, Park YS. Effects of Scutellaria baicalensis water extract on lipid metabolism and antioxidant defense system in rats fed high fat diet. J Korean Soc Food Sci Nutr. 2010 ; 39(2) :219-26. https://doi.org/10.3746/jkfn.2010.39.2.219
  10. Viollet B, Guigas B, Sanz Garcia N, Leclerc J, Foretz M, Andreelli F. Cellular and molecular mechanisms of metformin: an overview. Clin Sci. 2012 ; 122(6) : 253-70. https://doi.org/10.1042/CS20110386
  11. Levri KM, Slaymaker E, Last A, Yeh J, Ference J, Amico F. Metformin as treatment for overweight and obese adults: a systematic review. Ann Fam Med. 2005 ; 3(5) : 457-61. https://doi.org/10.1370/afm.343
  12. Balavoine GGA, Geletii YV. Peroxynitrite scavenging by different antioxidants. Part 1: convenient assay, Nitric Oxide. 1999 ; 3(1) : 40-54. https://doi.org/10.1006/niox.1999.0206
  13. Patel RP, McAndrew J, Sellak H, White CR, Jo H, Freeman BA, et al. Biological aspects of reactive nitrogen species. Biochim Biophys Acta. 1999 ; 1411(2-3) : 385-400. https://doi.org/10.1016/S0005-2728(99)00028-6
  14. Kahn BB, Flier JS. Obesity and insulin resistance. J Clin Invest. 2000 ; 106(4) : 473-81. https://doi.org/10.1172/JCI10842
  15. Seju C, Kyungsun H, Wang J, Chae H, Choi Y, Chin Y, et al. Effect of Atractylodes rhizoma alba, Houttuyniae herba, Lonicerae flos, Scutellariae radix and Coptis rhizoma extracts combined with metformin on the antioxidant and adipocyte differentiate inhibition. J Korean Med Obes Res. 2015 ; 15(1) :24-32. https://doi.org/10.15429/jkomor.2015.15.1.24
  16. Kim EK, Lee SH, Jhun JY, Byun JK, Jeong JH, Lee SY, et al. Metformin prevents fatty liver and improves balance of white/brown adipose in an obesity mouse model by inducing FGF21. Mediat Inflamm. 2016 ; 2016 : 5813030.
  17. Matsui Y, Hirasawa Y, Sugiura T, Toyoshi T, Kyuki K, Ito M. Metformin reduces body weight gain and improves glucose intolerance in high-fat diet-fed C57BL/6J mice. Biol Pharm Bull. 2010 ; 33(6) : 963-70. https://doi.org/10.1248/bpb.33.963
  18. Song KH, Lee SH, Kim BY, Park AY, Kim JY. Extracts of Scutellaria baicalensis reduced body weight and blood triglyceride in db/db mice. Phytother Res. 2013 ; 27(2) : 244-50. https://doi.org/10.1002/ptr.4691
  19. Moisan J, Brenton MC, Villeneuve J, Gregoire JP. Hypoglycemia-related emergency department visits and hypoglycemiarelated hospitalizations among new users of antidiabetes treatments. Can J Diabetes. 2013 ; 37(3) : 143-9. https://doi.org/10.1016/j.jcjd.2013.02.039
  20. Hirsh KR, Smith AE, Blue MN, Mock MG, Trexler ET. Influence of segmental body composition and adiposity hormones on resting metabolic rate and substrate utilization in overweight and obese adults. J Endocrinol Invest. 2017. doi:10.1007/s40618-017-0616-z.

피인용 문헌

  1. 최근 10년간 한방비만학회지의 연구동향 분석: 2010-2019년 한방비만학회지 게재논문을 중심으로 vol.20, pp.2, 2017, https://doi.org/10.15429/jkomor.2020.20.2.149