Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Characterization of Gibberellic Acid-Stimulated Arabidopsis (GASA) gene to drought stress response in Poplar (Populus alba × P. glandulosa)

현사시나무 Gibberellic Acid-Stimulated Arabidopsis (GASA) 유전자의 발현 특성 및 건조 스트레스 내성 구명

  • Choi, Hyunmo (Forest Biotechnology Division, National Institute of Forest Science) ;
  • Bae, Eun-Kyung (Forest Biotechnology Division, National Institute of Forest Science) ;
  • Choi, Young-Im (Forest Biotechnology Division, National Institute of Forest Science) ;
  • Yoon, Seo-Kyung (Forest Biotechnology Division, National Institute of Forest Science) ;
  • Lee, Hyoshin (Forest Biotechnology Division, National Institute of Forest Science)
  • 최현모 (국립산림과학원 산림생명공학과) ;
  • 배은경 (국립산림과학원 산림생명공학과) ;
  • 최영임 (국립산림과학원 산림생명공학과) ;
  • 윤서경 (국립산림과학원 산림생명공학과) ;
  • 이효신 (국립산림과학원 산림생명공학과)
  • Received : 2017.02.21
  • Accepted : 2017.03.21
  • Published : 2017.03.31
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Abstract

Gibberellic Acid-Stimulated Arabidopsis (GASA) genes are involved in plant hormone signaling, cell division and elongation, as well as in responses to stress conditions in plants. In this study, we isolated a GASA gene from hybrid poplar (Populus alba ${\times}$ P. glandulosa) and analyzed its physiological phenotype and molecular functions in poplar. PagGASA cDNA encodes a putative protein composed of 95 amino acids containing an N-terminal signal peptide and a conservative cysteine-rich C-terminal domain. Southern blot analysis revealed that one or two copies of the PagGASA are present in the poplar genome. The PagGASA transcripts were highly detected in flowers and roots. Moreover, the expression of PagGASA was induced by growth hormone (gibberellic acid) and stress hormones (abscisic acid, jasmonic acid, and salicylic acid). By using transgenic analysis, we showed that the upregulation of PagGASA in poplar provides high tolerance to drought stress. Therefore, our results suggest that PagGASA plays an important role in drought stress tolerance via stress-related plant hormone signaling in poplar.

GASA는 GA에 의해 조절되는 식물 유전자로서, 여러 식물에 보존되어 있고 다양한 조직에서 식물의 생장과 발달 및 스트레스 반응에 관여하는 것으로 알려져 있다. 본 연구에서는 GASA 유전자를 현사시나무(Populus alba ${\times}$ P. glandulosa)에서 분리하여 이를 PagGASA라 명명하고, 유전자의 구조와 발현특성을 조사하였다. PagGASA 유전자는 95개의 아미노산으로 구성된 단백질을 암호화하며, 아미노 말단에 시그널 펩티드 영역과 카르복시 말단에 12개 시스테인 잔기가 보존되어 있다. PagGASA는 현사시나무의 염색체에 1 ~ 2 copy 존재하며, 꽃과 뿌리에서 높게 발현하였다. 또한 PagGASA는 GA 뿐 아니라 ABA와 JA, SA와 같은 스트레스 관련 식물 호르몬의 처리에 의해서 발현이 증가하는 것으로 나타났다. 현사시나무에 형질전환하여 PagGASA를 과발현시킨 결과 건조 내성에 효과가 있음을 확인하였다. 따라서 PagGASA는 스트레스 관련 식물 호르몬 신호전달과 연결되어 건조 스트레스 방어기작에서 중요한 역할을 할 것으로 생각된다.

Keywords

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