The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Confirmation of Chestnut Powdery Mildew, Erysiphe castaneigena, in Korea with Morphological Characteristics and Molecular Analyses

형태적 특징 및 분자적 분석에 의한 밤나무 흰가루병균 Erysiphe castaneigena의 확인

  • Cho, Sung-Eun (Division of Environmental Science and Ecological Engineering, Korea University) ;
  • Lee, Sang-Hyun (Warm-Temperate and Subtropical Forest Research Center, National Institute of Forest Science) ;
  • Lee, Sun Keun (Division of Forest Diseases and Insect Pests, National Institute of Forest Science) ;
  • Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, Korea University)
  • 조성은 (고려대학교 환경생태공학부) ;
  • 이상현 (국립산림과학원 난대.아열대산림연구소) ;
  • 이선근 (국립산림과학원 산림병해충연구과) ;
  • 신현동 (고려대학교 환경생태공학부)
  • Received : 2017.04.14
  • Accepted : 2017.05.08
  • Published : 2017.06.01
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Abstract

The powdery mildew on Castanea crenata in South Korea was first recorded as Microsphaera alni in 1958. On the basis of its morphological characteristics and host range, the mildew was determined as Microsphaera sinensis in 1988. According to the rules of The International Code of Botanical Nomenclature, M. sinensis was renamed Erysiphe castaneigena in 2006. Nevertheless, taxonomic re-consideration of the morphological and molecular characteristics for the Castanea powdery mildew in Korea has not been performed. In the present study, we studied 34 powdery mildew samples collected from C. crenata in the Korea University Herbarium. On the basis of microscopic examinations of the holomorph and phylogenetic analysis of the internal transcribed spacer regions, we confirmed the powdery mildew fungus as E. castaneigena. In addition, sequence comparison between E. castaneigena and E. alphitoides ex Castanea sp. suggested a close phylogenetic affinity.

국내에서 밤나무 흰가루병균은 1958년에 Microsphaera alni로 처음 기록되었다. 1988년에는 이 흰가루병균의 형태적 특징과 기주를 고려하여 M. sinensis로 동정되었다. 이 학명은 '식물명명에 관한 국제규약'의 규칙에 따라 2006년에 E. castaneigena로 변경되었다. 그럼에도 불구하고, 국내의 밤나무 흰가루병균의 형태적 특징 및 분자적 분석에 의한 분류학적 재검토가 이루어지지 않았다. 본 연구에서는 고려대학교 진균표본실에 보존된 34점의 밤나무 흰가루병균 시료를 대상으로 수행되었다. 무성세대와 유성세대의 형태적 특징을 현미경으로 관찰하고 ITS 영역의 염기서열을 분석함으로써, 한국의 밤나무 흰가루병균은 E. castaneigena로 확인되었다. 또한, 염기서열 분석을 통하여 E. castaneigena와 밤나무 유래의 E. alphitoides는 계통학적으로 근연 관계임을 제시하였다.

Keywords

References

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