The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-Inflammatory Effect of Anemarrhenae Rhizoma 80% Ethanol Extract in RAW 264.7 cells

RAW 264.7 세포에서 지모(知母) 80% 에탄올 추출물의 항염증 효과

  • 이영근 (대전보건대학교 화장품과학과) ;
  • 김청택 ((주)알엔에스) ;
  • 최학주 (대전대학교 난치성면역질환의동서생명의학연구센터)
  • Received : 2017.04.11
  • Accepted : 2017.05.20
  • Published : 2017.05.30
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Abstract

Objective : According to recent studies, Anemarrhenae Rhizoma has anti-inflammatory activities of DW extract, but it hasn't not yet conducted to evaluate inflammatory factors about 80% ethanol extract. Therefore, The aim of this study is to investigate the various effects of individual or combined 80% ethanol extract of Anemarrhenae Rhizoma on cell viability and various anti-inflammatory factors. Methods : Anemarrhenae Rhizoma extract was prepared with 80% ethanol. MTT assay, ELISA, and Luminex were performed in LPS-activated RAW 264.7 cell line to measure cytotoxicity, Nitric oxide (NO), cyclooxygenase-2 (COX-2), prostaglandin E2 ($PGE_2$), Leukotriene B4 ($LTB_4$), and cytokines ($IL-1{\beta}$, IL-6, and $TNF-{\alpha}$), respectively. Results : At concentration of $200{\mu}g/m{\ell}$ Anemarrhenae Rhizoma extract, cytotoxicity was observed in RAW 264.7 cells. However, at concentration less than $100{\mu}g/m{\ell}$ of Anemarrhenae Rhizoma, cytotoxicity was not observed in RAW 264.7 cells. All concentration of Anemarrhenae Rhizoma extract showed no difference of NO, and $IL-1{\beta}$level in RAW 264.7 cells compared with control group. In contrast, at concentration of $100{\mu}g/m{\ell}$ Anemarrhenae Rhizoma extract significantly inhibited LPS-induced production of COX-2, PGE2, and $LTB_4$ level in RAW 264.7 cells. In addition, the production of proinflammatory cytokines (IL-6, $TNF-{\alpha}$) in LPS-induced RAW 264.7 cells was significantly decreased at concentration of all or 10, and $100{\mu}g/m{\ell}$, respectively. Conclusion : These findings demonstrate that Anemarrhenae Rhizoma has inhibitory effect on inflammatory mediators in LPS-activated RAW 264.7 cells showing possible developed as a raw material for new therapeutics to ease the symptoms related with inflammatory.

Keywords

References

  1. Kim YH. Pathology. Seoul: Hyunmoon. 2010 : 51.
  2. Cheon MS, Yoon TS, Choi GY, Kim SJ, Lee AY, Moon BC, Choo BK, Kim HK. Comparative Study of Extracts from Rhubarb on Inflammatory Activity in RAW 264.7 Cells. Korean J. Medicinal Crop Sci. 2009 ; 17(2) : 109-114.
  3. National College of Oriental Medicine co-edited a book. Herbal medicine. Seoul: Youngrimsa. 1995 : 161-3.
  4. Guojiazhongyixueguanliju. bianweihui. Zhonghuabencao. 8th ed. Shanghai: Shanghaikexuejishu Publisher. 1999 : 56-62.
  5. Jeong KK, Kang H, Myung EG, Shim BS, Kim SH, Choi SH, Ahn KS. Anti-inflammatory Effect of Anemarrhenae Rhizoma on Collagen Induced Arthritis - a Model for Rheumatoid Rrthritis in DBA/1J Mice and Cytokine Production in Raw 264.7 Cells. Korean J Oriental Physiol Pathol. 2008 ; 22(6) : 1416-22.
  6. Kim TH, Kim PH, Jeon BK, Yoon JR, Woo WH, Mun YJ, Lee JC, Lee BK, Lim KS. Effect of Anemarrhenae Rhizoma Ethanol Extract on Apoptosis Induction of HT-29 Human Colon Cancer Cells. The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology. 2011 ; 24(1) : 16-24.
  7. Yim CH, Cho JS, Kim HS, Kwon SM, Kim S, Kim IH, Park HS. The Growth Inhibition Effect of L-1210 and S-180 Cancer Cell Lines by the Extract from Anemarrhena Asphodeloides. Journal of Sasang Constitutional Medicine. 2007 ; 19(2) : 170-8.
  8. Kwon OJ, Lee HY, Kim TH, Kim SG. Antioxidant and pancreatic lipase inhibitory activities of Anemarrhena asphodeloides. Korean Journal of Food Preservation. 2014 ; 21(3) : 421-6. https://doi.org/10.11002/kjfp.2014.21.3.421
  9. Beak GH, Cho SJ, Cho HJ, Hong GH, Kim HW, Cho SI. Effects of Anemarrhenae Rhizoma on anti-oxidative activities. The Korea Journal of Herbology. 2007 ; 22(3) : 101-7.
  10. Kim JY, Yi YS, Lim YH. Biological and antifungal activity of herbal plant extracts against Candida species. Microbiology and Biotechnology Letters. 2009 ; 37(1) : 42-8.
  11. Doh ES. Antifungal activity of Anemarrhena asphodeloides, Coptis japonica and Phellodendron amurense extracts against Phytophthora blight. Korean J. Plant. Res. 1997 ; 10 : 351-9.
  12. Oh JK, Hyun SY, Oh HR, Jung JW, Park C, Lee SY, Park JH, Kim SY, Kim KH, Kim YK, Ryu JH. Effects of Anemarrhena asphodeloides on focal ischemic brain injury induced by middle cerebral artery occlusion in rats. Biological and Pharmaceutical Bulletin. 2007 ; 30(1) : 38-43. https://doi.org/10.1248/bpb.30.38
  13. Seo BW. Effects of Anemarrhenae Rhizoma on lipopolysaccharide-stimulated expression of cyclooxygenase-2 and inducible nitric oxide synthase in mouse BV2 microglial cells. Chungbuk: Semyung University; 2005.
  14. Kim BK. Anti-inflammatory effect of anemarrhena rhizome water extract on mouse macrophages. Gyeonggi-do : Gachon University; 2013.
  15. Yang PJ. Shennongbencaojingjiaozhu. Beijing: Xueyuan Publisher. 1998 : 134-5.
  16. Su SZ. Shangzhijunjixiao. Bencaotujing. Hefei: Anhuikexuejishu Publisher. 1994 : 163-4.
  17. Taohongjingji. Shangzhijunjixiao. Mingyibielv. Beijing: Renmin Publisher. 1986 : 122.
  18. Zhen QZ. Shangzhijunji. Yaoxinglun. Hefei: Anhuikejijishu Publisher. 2006 : 38.
  19. Lishizhenzhuan. Bencaogangmu. Hong Kong: Shiyongshuju. 1957 : 420-2.
  20. Ji JG. Anti-Oxidative and Anti-inflammatory Effect of Combined Extract and Individual Extract of GamiSaengmaeksan. The Korea Journal of Herbology. 2016 ; 31(1) : 69-75. https://doi.org/10.6116/kjh.2016.31.1.69.
  21. Posadas I, Terencio MC, Guillen I, Ferrandiz ML, Coloma J, Paya M, Alcaraz MJ. Co-regulation between cyclo-oxygenase-2 and inducible nitric oxide synthase expression in the time-course of murine inflammation. Naunyn-Schmiedeberg's archives of pharmacology. 2000 ; 361(1) : 98-106. https://doi.org/10.1007/s002109900150
  22. Lee H, Jung JY, Hwangbo M, Ku SK, Kim YW, Jee SY. Anti-inflammatory effects of Lespedeza cuneata in vivo and in vitro. The Korea Journal of Herbology. 2013 ; 28(4) : 83-92. https://doi.org/10.6116/kjh.2013.28.4.83
  23. Kim KA, Lee HS, Yoon HJ, Park SD. Anti-oxidative and Anti-inflammatory Effect of Fractionated Extracts of Cynomorium Songaricum. Korean J. Oriental Physiology & Pathology. 2009 ; 23(6) : 1320-31.
  24. Mantovani A. Cancer: inflaming metastasis. Nature. 2008 ; 457(7225) : 36-37.
  25. WEISZ A, CICATIELLO L, ESUMI H. Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-${\gamma}$, bacterial lipopolysaccharide and NG-monomethyl-L-arginine. Biochemical Journal. 1996 ; 316(1) : 209-15. https://doi.org/10.1042/bj3160209
  26. Canali R, Comitato R, Schonlau F, Virgili F. The anti-inflammatory pharmacology of Pycnogenol(R) in humans involves COX-2 and 5-LOX mRNA expression in leukocytes. International immunopharmacology. 2009 ; 9(10) : 1145-9. https://doi.org/10.1016/j.intimp.2009.06.001
  27. Gabay C. Interleukin-6 and chronic inflammation. Arthritis Research & Therapy. 2006 ; 8(2) : 1-3.
  28. Yun HY, Dawson VL, Dawson TM. Neurobiology of nitric oxide. Critical Reviews TM in Neurobiology. 1996 ; 10(4) : 291-316. https://doi.org/10.1615/CritRevNeurobiol.v10.i3-4.20