The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Inhibition Effects on Oxidative DNA Damage and Anti-inflammatory Effects of Nelumbinis Flos

연꽃의 산화적 DNA 손상 억제 활성 및 항염증 효과

  • Jeong, Hyung Jin (Department of Medicinal Plant Resources, Andong National University) ;
  • Park, Yeon Gyeong (Department of Medicinal Plant Science, Jungwon University) ;
  • Jang, Tae Won (Department of Medicinal Plant Science, Jungwon University) ;
  • Kim, Do Wan (Department of Food Science and Industry, Jungwon University) ;
  • Jeong, Jin Boo (Department of Medicinal Plant Resources, Andong National University) ;
  • Park, Jae Ho (Department of Medicinal Plant Science, Jungwon University)
  • 정형진 (안동대학교 생약자원학과) ;
  • 박연경 (중원대학교 생약자원개발학과) ;
  • 장태원 (중원대학교 생약자원개발학과) ;
  • 김도완 (중원대학교 식품공학과) ;
  • 정진부 (안동대학교 생약자원학과) ;
  • 박재호 (중원대학교 생약자원개발학과)
  • Received : 2017.04.14
  • Accepted : 2017.05.20
  • Published : 2017.05.30
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Abstract

Objective : Nelumbo nucifera, its rhizome and semen have been used as a traditional medicine which was studied on antioxidant, hepatoprotective effect, anti-obesity and the others. However, Nelumbinis Flos have not studied. We investigated protective effects on oxidative DNA damage and anti-inflammatory effects of Nelumbinis Flos. Methods : The antioxidant activity was conducted by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, 2, 2'-Azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) radical scavenging assay and reducing power assay. Total phenolic content was analyzed. Also, phenolic compounds were detected by HPLC/UV. The inhibitory effect on oxidative DNA damage was determined using ${\Phi}X-174$ RF I plasmid DNA cleavage assay. The anti-inflammatory effect of Nelumbinis Flos was measured by the amount of nitric-oxide (NO) produced and protein levels of iNOS, and COX-2 in LPS induced RAW 264.7 cells. Results : The results of DPPH and ABTS radical scavenging activity at $200{\mu}g/m{\ell}$ of extraction were $97.02{\pm}0.88%$ and $96.42{\pm}0.25%$. Reducing power (fold of L-ascorbic acid as control) was $100.14{\pm}0.31$ at $200{\mu}g/m{\ell}$. Total phenol content was $8.70{\pm}0.02mg/g$. Chlorogenic acid, catechin and epicatechin were found by HPLC. Nelumbinis Flos has inhibitory effect in dose-manner against oxidative DNA damage. In addition, it showed the anti-inflammatory effect by suppression of NO production as well as protein levels of iNOS, and COX-2. Conclusion : This study suggested that Nelumbinis Flos showed potential antioxidant and suppression activities of various factors were related in NO produced. Therefore, Nelumbinis Flos as natural plant resources that may help reduce inflammation and alleviate DNA damage.

Keywords

References

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