한국유기농업학회지 (Korean Journal of Organic Agriculture)

  • 제25권2호
  • /
  • Pages.295-310
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  • 2017
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  • 1229-3571(pISSN)
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  • 2287-819X(eISSN)
한국유기농업학회 (Korean Association of Organic Agriculture)
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참나무과 수목 5종 수용성 추출물의 항균활성

Antifungal Activity on the Water Extracts of Five Fagaceae Plants

  • 문상호 (제주대학교 농학과) ;
  • 송창길 (제주대학교 농학과) ;
  • 김태근 (제주대학교 농학과) ;
  • 오동은 (제주대학교 농학과) ;
  • 김현철 (제주특별자치도 세계유산.한라산연구원)
  • 투고 : 2016.09.23
  • 심사 : 2017.03.22
  • 발행 : 2017.05.31
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초록

본 연구는 자생식물 참나무과(Fagaceae)인 붉가시나무, 참가시나무, 종가시나무, 개가시나무, 구실잣밤나무 5종을 이용하여 친환경 농자재로 개발을 위한 기초자료를 제공하고자 수용성 추출액 농도에 따른 Colletotrichum gloeosporioides, Diaporthe citri, Phytophthora capsici 등 5종의 작물 병원균에 하여 생장을 조사하였다. 공여체식물별에 따른 수용성 추출액 농도가 증가됨에 따라 작물 병원균 균사의 생장이 감소하는 경향을 보이나 공여체식물과 병원균의 종류에 따라 억제의 정도차이를 보였다. 구실잣밤나무의 수용성 추출액인 경우 Diaporthe citri, Phytophthora capsici, Pythium graminicola에 대하여 25% 처리구에서 약 84%의 균사 생장을 억제하였고 50% 이상 처리구에서는 87%의 균사 생장을 억제하는 것으로 분석되었다. Diaporthe citri의 경우 붉가시나무 수용성 추출액에서는 균사의 생장을 억제하지 못하는 것으로 분석되었다. 참가시나무, 종가시나무, 개가시나무는 15% 내외로 미비하게 균사생장을 억제 되었다. 항균활성을 보이는 수용체식물의 총 페놀 함량은 구실잣밤나무 22.32 mg/g, 참가시나무 8.32 mg/g, 종가시나무 6.83 mg/g, 개가시나무 5.95 mg/g, 붉가시나무 5.24 mg/g 순으로 분석되었으며, 참나무과 5종 중 구실잣밤나무의 수용성 추출액이 가장 항균력이 우수한 것으로 판단된다.

This study investigated the growth of five phytopathogenic fungi including Colletotrichum gloeosporioides, Diaporthe citri, Phytophthora capsici and others according to different concentrations of water extract in order to provide reference data for developing environment-friendly agricultural materials using five native Fagaceae species including Quercus acuta, Quercus salicina, Quercus glauca, Quercus gilva and Castanopsis cuspidata var. sieboldii. As the concentration of aqueous extracts of Fagaceae increased according to donor plants, the mycelial growth of phytopathogens showed a decreasing tendency. Differences were found in the degree of inhibition according to types of donor plants and pathogenic fungi. Diaporthe citri, Phytophthora capsici, Pythium graminicola on the water extract of Castanopsis cuspidata var. sieboldii inhibited mycelial growth by 84% in 25% of the treatment group and by 87% in more than half of the treatment group. The water extract of Quercus acuta was found to have no inhibitory effect against the mycelial growth of Diaporthe citri. The aqueous extracts of Quercus salicina, Quercus glauca and Quercus gilva insignificantly inhibited mycelial growth by approximately 15%. The total phenolic content of receptor plants exhibiting antifungal activity was highest in Castanopsis cuspidata var. sieboldii with a content of 22.32 mg/g phenols, followed by Quercus salicina with 8.32 mg/g, Quercus glauca with 6.83 mg/g, Quercus gilva with 5.95 mg/g, and Quercus acuta with 5.24 mg/g. The aqueous extracts of Castanopsis cuspidata var. sieboldii among the five Five Fagaceae Plants of were the most effective antifungal activity.

키워드

참고문헌

  1. Arnason, J. T., B. J. R. Philogene, P. Morand, K. Imrie, S. Iyengar, F. Duval, C. Soucy Breau, J. V. Scaiano, N. H. Werstiuk, B. Hasspieler, and A. E. R. Downe. 1989. Naturally occurring and synthetic thiopenes as photoactivated insecticides. In Insecticides of Plant Orgin. (Eds. J. T. Arnason, B. J. R. Philogene and P. Morand) ACS symposium series no. 387, American Chemical Society, Washington, DC. pp. 164-172.
  2. Chai, M. L., J. M. Lee, M. H. Park, and D. H. Kim. 2001. In vitro selection for large patch resistance in zoysiagrass. J. Kor. Soc. Hort. Sci. 42: 249-253.
  3. Chang, C. S. 2007. Fagaceae Dumort. The Genera of Vascular Plants of Korea. Academy Publishing. Co. Seoul, 1268-1274.
  4. Choi, G. B., S. B. Woo, J. Y. Song, J. H. Kang, T. K. Kim, H. C. Kim, and C. K. Song. 2015. Herbicidal and Antifungal Activities of the aqueous extracts of Persicaria longiseta. Korean J. Org. Agric. 23(3): 481-495. https://doi.org/10.11625/KJOA.2015.23.3.481
  5. Clark, A. M., F. S. EI Feraly, and W. S. Li. 1981. Antimicrobial activity of phenolic constituent of Mangolina grandiflora L. J. Pharm. Sci., 70: 951-952. https://doi.org/10.1002/jps.2600700833
  6. Darley Hill, S. and W. C. Johnson. 1981. Acorn dispersal by blue jay (Cyanocitta cristata). Oecologia 50: 231-232. https://doi.org/10.1007/BF00348043
  7. Geon, M. G., I. S. Kim, S. C. Lee, T. K. Son, and G. Y. Shim. 2005. Effects of pyroligneous acid on control of large patch in zoysiagrass. Kor. Turfgrass Sci. 19: 73-83.
  8. Graham, H. D. 1992. Modified prussian blue assay for total phenol compound. J. Agric. Food Chem. 40: 801-807. https://doi.org/10.1021/jf00017a018
  9. Han, B. H., J. Y. Kim, I. T. Choi, and K. J. Lee. 2007. Vegetation sturcure of evergreen broad-leaved forest in Dongbaekdongsan (Mt.), Jeju-Do, Korea. Kor. J. Env. Eco. 21(4): 336-346.
  10. Hong M. G., Y. H. Chung, and J. O. Hong. 1988. Development of Vegetable Fungicide for Valsa ceratosperma. Collected Papers for Farming (Crop Protection). 30(3): 24-30.
  11. Horsley, S. B. 1977. Allelopathic interference among plants. II. Physiological modes of action. In H. E. Wilcox and A. F. Hamer (eds.). Proceedings of the 4th Nothern American Forest Biology Workshop. School of Continuing Education, College of Environmental Science and Forestry, Syracuse. pp. 93-136.
  12. Huang, C. C., Y. C. Chang, and B. Bartholomew. 1999. Fagaceae. In Wu, C. Y & Raven P. H. (eds.), Flora of China. Sci. Press, Beijing & Missouri Bot. Garden Press, St. Louis 4: 380-400.
  13. Huang, S., Z. Zhang, Y. Li, and H. Xu. 2010. Anti-Insect Activity of the Methanol Extracts of Fern and Gymnosperm. Agricultural Science in China. 9(2): 249-256. https://doi.org/10.1016/S1671-2927(09)60090-0
  14. Hyoun, D. G., J. Y. Song, T. K. Kim, D. C. Jung, S. C. Song, Y. S. Kang, J. W. Cha, H. S. Lee, Y. H. Yang, H. C. Kim, and C. K. Song. 2014. Herbicidal and Antifungal Activities of the aqueous extracts of Several Naturalized Plants. Korean J. Org. Agric. 22(2): 303-319. (in Korean). https://doi.org/10.11625/KJOA.2014.22.2.303
  15. Hyun, J. W., D. H. Kim, K. S. Kim, S. C. Lee, S. W. Ko, and H. C. Lim. 2004. Disease and the Symptoms Recently Occurred on 'Shiranuhi' Citrus Cultivar in Jeju Island. Res. Plant Dis. 10(2): 94-99. https://doi.org/10.5423/RPD.2004.10.2.094
  16. Islam, M. R., Y. T. Jeong, Y. J. Ryu, C. H. Song, and Y. S. Lee. 2009. Isolation, identification and optimal culture conditions of Streptomyces albidoflavus C247 producing antifungal agents against Rhizoctonia solani AG2-2. Mycobiology 37: 114-120. https://doi.org/10.4489/MYCO.2009.37.2.114
  17. Jung, W. C., T. S. Shin, B. S. Kim, J. S. Im, J. H. Lee, and J. W. Kim. 2008. Efficacy of antagonistic bacteria for bacteria for biological control of Rhizoctonia bright (large patch) on zoysiagrass. Res. Plant dis. 14:43-50. https://doi.org/10.5423/RPD.2008.14.1.043
  18. Kang, J. H., D. H. Kim, D. G. Lee, I. S. Kim, M. G. Jeon, I. H. Kim, and S. H. Lee. 2013. Screening of Antifungal Activities of Medicinal Plants for the Control of Turfgrass Fungal Disease. Weed Turf. Sci. 2(1): 70-75. https://doi.org/10.5660/WTS.2013.2.1.070
  19. Kim, C. H. and K. S. Park. 1988. A predictive model of disease progression of red-pepper anthracnose. Kor. J. Plant Pathol. 4: 325-331.
  20. Kira, T. 1991. Forest ecosystems of east and south-east Asia in global perspective. Ecol. Res. 6: 185-200. https://doi.org/10.1007/BF02347161
  21. Ko, M. S. and J. B. Yang. 2011. Antioxidant and Antimicrobial Activities of Smilax china Leaf Extracts. Korean J. Food Preserv. 18(5): 764-772. https://doi.org/10.11002/kjfp.2011.18.5.764
  22. Kong Y. J., G. P. Hong, H. J. Kwon, J. K. Hong, B. K. Park, and D. H. Oh. 2001. Antimicrobial activities of Quercus spp. leaf ethanol extract against foodborne disease microorganism. J. Korean Soc. Food Sci. Nutr. 30(3): 415-420.
  23. Lee, W. C. 1996. Lineamenta Florae Koreae. Academic Press, Seoul.
  24. Lee, Y. S. 1997. Biological control of soilborne pathogenic fungi of grasses. Korea Science and Engineering Foundation, Daejeon, Korea.
  25. Lim. J. S., H. S. Mo, E. H. Lee, K. C. Park, and C. M. Chung. 2014. Suppressive Effects of Homemade Environment-friendly Materials on Alternaria Blight and Anthracnose of Ginseng. Korean J. Org. Agric. 22(4): 705-718. https://doi.org/10.11625/KJOA.2014.22.4.705
  26. Liu, R. H. 2004. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J. Nutr. 134: 3479-3485. https://doi.org/10.1093/jn/134.12.3479S
  27. Nixon, K. C. 2006. Global and neotropical distribution and diversity of Oak (genus Quercus) and oak forests. Ecologica and Conservation of Neotropical Montane Oak Forests 3-13. Springer Berlin Heidelberg.
  28. Oh, K. K., J. G. Kon, and T. H. Kim. 2007. Altitudinal distribution of plant communities at Donnaeko valley in the Mt. Hallasan. Kor. J. Env. Eco. 21(2): 141-148.
  29. Park, D. S., K. Kohmoto, and M. Katayama. 1986. Isolation and Identification of Antifungal Fatty Acids from the Extract of Common Purslane (Portulaca oleracea L.). Journal of Korea Society of Plant Pathology. 2(2): 82-88.
  30. Park, J. H., K. Y. Ryu, H. J. Jee, B. M. Lee, S. B. Paik, and S. H. Kyeong. 1990. Research of Antagonistic Plants and Development of Application Technology for Control of Gray Mold Disease (II). Collected Papers on Agricultural Experimentations and Researches (Academic-Agricultural Industry Cooperation). 33: 29-134.
  31. Park, K. S. and C. H. Kim. 1992. Identification, distribution and etiological characteristics of anthracnose fungi of red pepper in Korea. Kor. J. Plant Pathol. 8: 61-69.
  32. Rice. E. L. 1979. Allelopathy-An update. The Botanical Review 45: 15-109. https://doi.org/10.1007/BF02869951
  33. Rice, E. L. 1984. Allelopathy 2nd ed. Academic Press, inc., Orlando, Florida. pp. 1-7.
  34. Rizvi. S. J. H., H. Haque, V. K. Singb, and V. Rizvi. 1992. A discipline called allelopathy. in: Allelopathy. Rizvi. S. J. H and V. Rizvi.. Chapman and Hall. Hong-Kong. pp. 1-10.
  35. Robbins, C. T., T. A. Hanley, A. E. Hagerman, O. Hjeljord, D. L. Baker, C. C. Schwartz, and W. W. Mautz. 1987. Role of tannins in defending plants against ruminants reduction in protein availability. Ecology 68:98-107. https://doi.org/10.2307/1938809
  36. Putnam. A. R. and C. H. Tang. 1986. The science of allelopathy. John Wiley & Sons. Canada. pp. 174-187.
  37. Ryu, S. W., C. W. Jin, H. S. Lee, J. Y. Lee, K. Sapkota, B. G. Lee, C. Y. Yu, M. K. Lee, M. J. Kim, and D. H. Cho. 2006. Changes in total polyphenol, total flavonoid contents and antioxidant activities of Hibiscus cannabinus L. Kor. J. Med. Crop. Sci. 14: 307-310.
  38. Saxena, R. C. 1989. Insecticides from neem. In insecticides of plant origin (J. T. Arnason, B. J. R. Philogene, and P. Morand, eds.). ACS Symp. Ser. No. 387. Am. Chem. Soc. Washington, D.C. pp. 110-135.
  39. Shim, G. Y., J. W. Kim, and H. K. Kim. 1994. Occurrence of Rhizoctonia blight of zoysiagrasses in golf courses in Korea. Korean J. Plant Pathol. 10: 54-60.
  40. Shimada, T. and T. Saitoh. 2003. Negative effacts of acorns on the wood mouse Apodemus speciosus. Popul. Ecol. 45:7-17.
  41. Song, G. P. 2007. The flora and vegetation of evergreen broadleaved forest zone on eastfacing and west-facing slopes of Mt. Halla. Ph. D. dissertation. Jeju National University, Jeju, Korea.
  42. Uddin, W. and G. Viji. 2002. Biological control of turfgrass disease. pp. 313-314. In: Gnanamanckam (ed.). Biological control of crop diseases. Marcel Dekker, Inc. Barsel, New.
  43. Wink, M. 1993. Production and application of phytochemicals from an agricultural perspective. In Phytochemistry and Agriculture (van Beek TA, Breteler H. eds.). Clarendon Press, Oxford, pp. 171-213.
  44. Yang, J. S., S. Y. Kang, and Y. C. Jeun. 2014. Suppression of Citrus Canker by Pretreatment with Rhizobacterial Strains Showing Antibacterial Activity. Res. Plant Dis. 20(2): 101-106. https://doi.org/10.5423/RPD.2014.20.2.101
  45. Yin, J. F., W. H. Zhang, J. Q. Li, Y. H. Li, H. L. Hou, and X. Y. Zhou. 2007. Screening and antagonistic mechanism of biocontrol agents against Phytophthora blight of pepper. Acta Phytopathologica Sinica. 37: 88-94. York. USA.
  46. Yun, J. H., T. Hukusima, M. H. Kim, and M. Yoshikawa. 2011. The comparative study on the distribution and species composition of forest community in Korea and Japan around the ease sea. Kor. J. Eco. 25(3): 327-357.