Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Extracts of Desmodii Herba Suppresses of Rheumatoid Arthritis

류마티스 관절염에서 광금전초 추출물의 관절염 억제 효과

  • Noh, Eun Mi (Center for Metabolic Function Regulation, Wonkwang University School of Medicine) ;
  • Song, Hyun Kyung (Center for Metabolic Function Regulation, Wonkwang University School of Medicine) ;
  • Kim, Jeong Mi (Center for Metabolic Function Regulation, Wonkwang University School of Medicine) ;
  • Lee, Guem San (Department of Herbology, School of Korean Medicine, Wonkwang University) ;
  • Kwon, Kang Beom (Center for Metabolic Function Regulation, Wonkwang University School of Medicine) ;
  • Lee, Young Rae (Center for Metabolic Function Regulation, Wonkwang University School of Medicine)
  • 노은미 (원광대학교 의과대학 대사질환연구센터) ;
  • 송현경 (원광대학교 의과대학 대사질환연구센터) ;
  • 김정미 (원광대학교 의과대학 대사질환연구센터) ;
  • 이금산 (원광대학교 한의과대학 본초학교실) ;
  • 권강범 (원광대학교 의과대학 대사질환연구센터) ;
  • 이영래 (원광대학교 의과대학 대사질환연구센터)
  • Received : 2017.07.17
  • Accepted : 2017.11.15
  • Published : 2017.12.25
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Abstract

Desomodii Herba (DH) has been shown to exhibit pharmacologyical activities, such as increase myocaridal contraction and secretion of hepatic bile. DH is used to reduce pain caused by rheumatoid arthritis(RA) in Korean medicine. However, the DH exact(DHE) effect and mechanism on rheumatoid arthritis are unknown. In this study, we aimed at the inhibitory effect of DHE on rheumatoid arthritis, and investigated the effect in collagen-induced mice arthritis model and TNF-${\alpha}$ induced MMP-1 and MMP-3 expression including the molecular basis in rheumatoid arthritis synovial fibroblasts (RASFs).The effect of DHE on RA was measured by clinical scoring system. In RASFs, expression of MMP-1 and MMP-3 was assessed by Western blotting and real-time PCR. Also, Western blotting used to evaluate the phosphorylation levels of p38, ERK and JNK and activation of NF-${\kappa}B$ and AP-1. Our results showed that DHE reduced collagen-induced arthritis in mice. DHE inhibits TNF-${\alpha}$ induced MMP-1 and MMP-3 expression and mRNA levels in RASFs. The inhibitory effect of DHE was mediated by the inhibition of the AP-1/JNK signaling pathway. Taken together, our results suggest that the DHE may have preventive potential for rheumatoid arthritis.

Keywords

References

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