Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of Curcuminoids in Engineered Escherichia coli

  • Kim, Eun Ji (Department of Integrative Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University) ;
  • Cha, Mi Na (Department of Integrative Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University) ;
  • Kim, Bong-Gyu (Department of Forest Resources, Gyeongnam National University of Science and Technology) ;
  • Ahn, Joong-Hoon (Department of Integrative Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University)
  • Received : 2017.01.13
  • Accepted : 2017.03.09
  • Published : 2017.05.28
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Abstract

Curcumin, a hydrophobic polyphenol derived from the rhizome of the herb Curcuma longa, possesses diverse pharmacological properties, including anti-inflammatory, antioxidant, antiproliferative, and antiangiogenic activities. Two curcuminoids (dicinnamoylmethane and bisdemethoxycurcumin) were synthesized from glucose in Escherichia coli. PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS (curcumin synthase) genes, were introduced into E. coli, and each strain produced dicinnamoylmethane or bisdemethoxycurcumin, respectively. In order to increase the production of curcuminoids in E. coli, the shikimic acid biosynthesis pathway, which increases the substrates for curcuminoid biosynthesis, was engineered. Using the engineered strains, the production of bisdemethoxycurcumin increased from 0.32 to 4.63 mg/l, and that of dicinnamoylmethane from 1.24 to 6.95 mg/l.

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References

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