동아시아식생활학회지 (Journal of the East Asian Society of Dietary Life)

  • 제27권2호
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  • Pages.235-242
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  • 2017
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  • 1225-6781(pISSN)
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  • 2288-8802(eISSN)
동아시아식생활학회 (The East Asian Society of Dietary Life)
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감국(Chrysanthemum indicum L.) 추출물과 분획물의 항산화 및 α-Glucosidase와 PTP1B 저해 활성

Antioxidant Activity and Inhibition Activity against α-Glucosidase and PTP1B of Chrysanthemum indicum L. Extract and Fractions

  • 황성우 (원광대학교 한약학과) ;
  • 이훈연 (원광대학교 한약학과) ;
  • 차지윤 (원광대학교 한약학과) ;
  • 김보람 (원광대학교 한약학과) ;
  • 박민 (원광대학교 한약학과) ;
  • 이보리 (원광대학교 한약학과) ;
  • 문광현 ((재)순창건강장수연구소) ;
  • 이정호 ((재)순창건강장수연구소) ;
  • 김대근 ((재)순창건강장수연구소) ;
  • 김욱 ((재)순창건강장수연구소) ;
  • 정경옥 ((재)순창건강장수연구소) ;
  • 이영미 (원광대학교 한약학과)
  • Hwang, Sung-Woo (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Cha, Ji-Yun (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Lee, Hoon-Yeon (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Kim, Bo-Ram (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Park, Min (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Lee, Bo-Ri (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Moon, Kwang- Hyun (Sunchang Research Institute of Health and Longevity) ;
  • Lee, Jeong-Ho (Sunchang Research Institute of Health and Longevity) ;
  • Kim, Dae-Geun (Sunchang Research Institute of Health and Longevity) ;
  • Kim, Wook (Sunchang Research Institute of Health and Longevity) ;
  • Jeong, Kyoung-Ok (Sunchang Research Institute of Health and Longevity) ;
  • Lee, Young-Mi (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
  • 투고 : 2017.03.08
  • 심사 : 2017.04.18
  • 발행 : 2017.04.30
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초록

본 연구에서는 국화과에 속하는 Chrysanthemum indicum L.(CI)의 70% EtOH 추출물 및 분획물(dichloromethane, ethyl acetate 및 H2O)의 폴리페놀 함량, 플라보노이드 함량, 항산화 활성 및 생리활성을 평가하였다. 항산화 활성으로는 DPPH, ABTS 및 superoxide 소거 활성을 확인하였고, 생리활성 평가는 ${\alpha}$-glucosidase와 PTP1B 저해 활성을 측정하였다. 그 결과, CI 70% EtOH 추출물에서 폴리페놀과 플라보노이드의 함량은 각각 30.20 mg 및 31.58 mg인 것으로 확인되었다. 여러 분획물 중 EA 분획물이 폴리페놀(87.87 mg)과 플라보노이드(65.12 mg)의 함량이 가장 높았다. EA 분획물의 DPPH, ABTS 및 SOD 소거능은 각각 93.84%, 93.38% 및 84.68%의 억제를 나타냈다. EA 분획물은 70% EtOH 추출물 및 다른 분획과 비교하여 가장 높은 항산화 활성을 보였다. 또한 EA 분획물($20{\mu}g/mL$)의 ${\alpha}$-glucosidase 및 PTP1B 억제 결과는 다른 시료에 비해 각각 30.29% 및 99.24%의 가장 높은 억제를 나타내었다. 본 연구 결과를 통해 감국의 EA 분획물이 70% EtOH 추출물 및 다른 분획물보다 많은 항산화 및 항당뇨 성분을 함유하는 것으로 보여진다. 결론적으로 우리는 CI의 천연 항산화 물질 및 기능성 물질에 성능이 좋아 화장품 첨가제나 음료로도 개발이 가능할 수 있을 것으로 사료된다.

In this study, we evaluated the polyphenol content, flavonoid content, antioxidant and biological of 70% EtOH extract and its fractions (dichloromethane, ethyl acetate and water) of the plant Chrysanthemum indicum L. (CI) belonging to family chrysanthemum. antioxidant activity was determined 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and superoxide scavenging activity. The biological activity tests included measurement of ${\alpha}$-glucosidase and PTP1B. The results show that content of polyphenol and flavonoid in 70% EtOH of CI 30.20 mg and 31.58 mg, respectively. Among the different samples, ethyl acetate (EA) fraction showed the highest level of polyphenol (87.87 mg) and flavonoid (65.12 mg). The DPPH, ABTS and superoxide dismutase(SOD) antioxidant assay of EA fraction showed 93.84%, 93.38% and 84.68% inhibition respectively. The EA fraction also showed the highest antioxidant activity compared to 70% EtOH extract and other fractions. EA fraction ($20{\mu}g/mL$) showed the highest 30.29% and 99.24%, respectively compared to other samples. The results of this study show that the EA fraction contained more antioxidant and antidiabetic components than 70% EtOH extract and other fractions. In conclusion, we believe that CI may be useful natural antioxidant and functional material.

키워드

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피인용 문헌

  1. Chrysanthemum indicum Inhibits Adipogenesis and Activates the AMPK Pathway in High-Fat-Diet-Induced Obese Mice vol.46, pp.1, 2017, https://doi.org/10.1142/s0192415x18500076
  2. 매실 순차분획물의 용매별 항산화 활성 및 α-glucosidase 억제 효과 vol.29, pp.10, 2017, https://doi.org/10.5352/jls.2019.29.10.1111