Molecules and Cells

  • 제40권4호
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  • Pages.307-313
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  • 2017
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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Caloric Restriction-Induced Extension of Chronological Lifespan Requires Intact Respiration in Budding Yeast

  • Kwon, Young-Yon (Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Sung-Keun (Department of Pharmacology, College of Medicine, Inha University) ;
  • Lee, Cheol-Koo (Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University)
  • 투고 : 2016.11.16
  • 심사 : 2017.04.07
  • 발행 : 2017.04.30
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초록

Caloric restriction (CR) has been shown to extend lifespan and prevent cellular senescence in various species ranging from yeast to humans. Many effects of CR may contribute to extend lifespan. Specifically, CR prevents oxidative damage from reactive oxygen species (ROS) by enhancing mitochondrial function. In this study, we characterized 33 single electron transport chain (ETC) gene-deletion strains to identify CR-induced chronological lifespan (CLS) extension mechanisms. Interestingly, defects in 17 of these 33 ETC gene-deleted strains showed loss of both respiratory function and CR-induced CLS extension. On the contrary, the other 16 respiration-capable mutants showed increased CLS upon CR along with increased mitochondrial membrane potential (MMP) and intracellular adenosine triphosphate (ATP) levels, with decreased mitochondrial superoxide generation. We measured the same parameters in the 17 non-respiratory mutants upon CR. CR simultaneously increased MMP and mitochondrial superoxide generation without altering intracellular ATP levels. In conclusion, respiration is essential for CLS extension by CR and is important for balancing MMP, ROS, and ATP levels.

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참고문헌

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