Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effects of hot water extracts of roasted radish against renal oxidative stress induced by high-fat diet

고지방식사로 유도된 신장 산화스트레스를 개선하는 가압볶음 무말랭이 열수추출물 효과

  • Jeon, Yeonhui (Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University) ;
  • Kim, Mijeong (Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University) ;
  • Han, Seongkyung (Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University) ;
  • Song, Yeong-Bok (Sejeon Co., Ltd.) ;
  • Song, Yeong Ok (Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University)
  • 전연희 (부산대학교 식품영양학과 및 김치연구소) ;
  • 김미정 (부산대학교 식품영양학과 및 김치연구소) ;
  • 한성경 (부산대학교 식품영양학과 및 김치연구소) ;
  • 송영복 ((주)세전식품연구소) ;
  • 송영옥 (부산대학교 식품영양학과 및 김치연구소)
  • Received : 2016.09.24
  • Accepted : 2016.12.12
  • Published : 2017.04.30
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Abstract

The antioxidant and anti-inflammatory effects of roasted dried radish (RDR) against renal oxidative stress were examined in high-fat diet (HFD)-fed mice. The HFD was prepared by adding lard to chow diet to provide 50% of the calories from fat. Hot water extracts of dried radish (DR) or RDR were administered orally to mice at 237 mg/kg bw/day, whereas distilled water was administered as a vehicle for 12 weeks. Compared to the control group, renal reactive oxygen species, peroxynitrite, and thiobarbituric acid reactive substance level in the DR or RDR group were significantly decreased, whereas the glutathione level was increased (p<0.05). Protein expressions of antioxidant factors such as nuclear factor erythroid 2-related factor-2, heme oxygenase-1, glutathione S-transferase, superoxide dismutase, catalase, and glutathione peroxidase were significantly increased in the DR and RDR groups; however, nuclear factor-kappa B expression was suppressed (p<0.05). These antioxidant and anti-inflammatory effects of RDR were found to be significantly greater than those of DR.

고지방식사를 섭취한 쥐의 신장에서 가압볶음 무말랭이 추출물의 산화방지, 항염증 효과를 확인하였다. 실험군은 chow diet와 증류수를 경구 투여하는 NOR group, 고지방식사와 증류수를 경구 투여하는 CON group, 고지방식사와 237 mg/kg bw/day농도의 DR과 RDR를 각각 경구 투여하는 DR group, RDR group으로 나누어 12주간 사육하였다. 신장의 산화스트레스 지표인 ROS, $ONOO^-$, 그리고 지방질과산화물의 농도를 확인한 결과 CON group이 NOR group에 비해 유의적으로 증가하였다. 반면 DR group과 RDR group에서 유의적으로 감소하였다. 체내 산화방지 지표인 글루타싸이온의 농도와 산화방지단백질의 발현은 DR group과 RDR group 모두 CON group에 비해 증가하였다. NF-${\kappa}B$ 발현은 CON group이 NOR group에 비해 증가하였으나 DR group과 RDR group은 모두 감소하였다. RDR group은 DR group에 비해 $ONOO^-$ 및 지방질과산화물 농도가 감소하였고 글루타싸이온 농도와 산화방지 관련 단백질 발현 중 Nrf2, HO-1, 카탈레이스, 그리고 GPx가 증가하였고 염증반응 전사인자인 NF-${\kappa}B$의 발현이 낮아졌다. RDR group의 산화스트레스 개선효과는 유의적으로 높은 것으로 확인되었다. 따라서 가압볶음무말랭이를 차로 제조하여 섭취할 때 고지방식사로 유도된 신장의 산화스트레스를 억제할 수 있을 것으로 사료되며, 그 효과는 무말랭이보다 높은 것으로 확인되었다.

Keywords

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