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Determination of Carbendazim in Commercial Agricultural Products by LC-MS/MS

LC-MS/MS를 이용한 농산물 중 카벤다짐 분석

  • Hwang, Lae-Hwong (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Sung-Deuk (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Jeong-Gon (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Ji-Young (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Park, So-Hyun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Ji-Hae (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Park, Jung-Hyun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Han, Chang-Ho (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Mu-Sang (Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 황래홍 (서울특별시 보건환경연구원) ;
  • 이성득 (서울특별시 보건환경연구원) ;
  • 김정곤 (서울특별시 보건환경연구원) ;
  • 김지영 (서울특별시 보건환경연구원) ;
  • 박소현 (서울특별시 보건환경연구원) ;
  • 김지혜 (서울특별시 보건환경연구원) ;
  • 박정현 (서울특별시 보건환경연구원) ;
  • 한창호 (서울특별시 보건환경연구원) ;
  • 김무상 (서울특별시 보건환경연구원)
  • Received : 2016.12.16
  • Accepted : 2017.02.15
  • Published : 2017.04.30

Abstract

A rapid and precise method using LC-MS/MS was developed for carbendazim analysis in agricultural products. This compound was extracted with acetonitrile from agricultural products and cleaned up by solid-phase extraction procedure. The limit of detection and quantification were 0.001 mg/kg and 0.004 mg/kg, respectively. The mean recoveries and precision from 4 agricultural products, soybean sprout and mungbean sprout were in the range of 83.3-86.4% and 0.2-3.0% spiked at 1.0 mg/kg and those were in the range of 77.3-90.1% and 1.3-3.8% spiked at 0.02 mg/kg. The present method is faster and more precise compared with the multi-residue method of Korean Food Code. Therefore, we conclude that this method is suitable for carbendazim determination in a wide range of agricultural products.

LC-MS/MS를 이용하여 신속하고 정밀한 카벤다짐 분석법을 연구하였다. 농산물 중 카벤다짐은 acetonitrile로 추출한 후 SPE cartridge을 이용하여 전처리를 수행하였으며, 최소 검출한계 및 정량한계는 각각 0.001과 0.004 mg/kg이었다. 농산물에 각각 1.0 mg/kg 및 0.02 mg/kg 농도의 카벤다짐을 첨가하여 분석 시 회수율과 정밀성은 각각 상추가 84.9%, 1.0% 및 87.8%, 3.8% 얼갈이배추가 83.4%, 1.5% 및 86.8%, 2.7% 양배추가 86.4%, 2.0% 및 77.3%, 1.5% 근대가 83.3%, 0.2% 및 86.8%, 2.0 % 이었으며, 콩나물은 85.9%, 3.0% 및 90.1%, 1.3%, 그리고 숙주나물이 83.3%, 2.0% 및 85.9%, 1.9% 로 조사되었다. 본 연구의 시험법은 식품공전의 다성분분석법에 비해 신속하고 정밀한 분석법으로 농산물에 대한 카벤다짐 분석에 적합한 것으로 조사되었다.

Keywords

References

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