Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Evaluation of antimicrobial activity and efficacy of herbal oils and extracts in disinfection of gutta percha cones before obturation

  • Makade, Chetana S. (Department of Conservative Dentistry and Endodontics, VSPM Dental College & Research Centre) ;
  • Shenoi, Pratima R. (Department of Conservative Dentistry and Endodontics, VSPM Dental College & Research Centre) ;
  • Morey, Elakshi (Department of Conservative Dentistry and Endodontics, VSPM Dental College & Research Centre) ;
  • Paralikar, Ameya V. (Department of Conservative Dentistry and Endodontics, VSPM Dental College & Research Centre)
  • Received : 2016.07.22
  • Accepted : 2017.07.02
  • Published : 2017.11.08
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Abstract

Objectives: Literature has shown that micro-organisms contaminate gutta percha (GP) during storage and manipulation. Till date herbal extracts are not explored as an alternative medicament for pre-operative chairside disinfection of GP cones. The purpose of our study was to evaluate the antimicrobial activity and efficacy of lemon grass oil (LG), basil oil (BO), and obicure tea extract (OT) in disinfecting GP cones before obturation. Materials and Methods: Agar diffusion method was used to evaluate the antimicrobial efficacy of LG, BO, OT, and sodium hypochlorite (control) against common contaminants, namely, Enterococcus faecalis, Staphylococcus aureus, and Candida albicans. One hundred and twenty GP cones were contaminated and cut into 2. First half was placed in the broth and incubated; whereas the second was treated with herbal extracts for 1 minute and then incubated for 24 hours in the broth. Any inhibition in bacterial growth was noted with presence/absence of turbidity. Two-way analysis of variance and ${\chi}^2$ test were used to assess the effectiveness of herbal extracts to decontaminate GP. Results: LG showed the highest inhibition zones ($29.9{\pm}6.9mm$) for all tested organisms, followed by OT extract ($16.3{\pm}1.8mm$), sodium hypochlorite ($16.0{\pm}1.6mm$), and BO ($14.5{\pm}5.3mm$). Statistically significant difference was observed between LG and other herbal extracts (p < 0.05). Conclusions: All extracts proved to be potential rapid chairside disinfectants of GP cones with LG showing the highest antimicrobial activity.

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References

  1. Siqueira JF Jr, da Silva CH, Cerqueira M das D, Lopes HP, de Uzeda M. Effectiveness of four chemical solutions in eliminating Bacillus subtilis spores on gutta-percha cones. Endod Dent Traumatol 1998;14:124-126. https://doi.org/10.1111/j.1600-9657.1998.tb00824.x
  2. Moorer WR, Genet JM. Evidence for antibacterial activity of endodontic gutta-percha cones. Oral Surg Oral Med Oral Pathol 1982;53:503-507. https://doi.org/10.1016/0030-4220(82)90467-4
  3. Higgins JR, Newton CW, Palenik CJ. The use of paraformaldehyde powder for the sterile storage of gutta-percha cones. J Endod 1986;12:242-248. https://doi.org/10.1016/S0099-2399(86)80255-2
  4. da Motta PG, de Figueiredo CB, Maltos SM, Nicoli JR, Ribeiro Sobrinho AP, Maltos KL, Carvalhais HP. Efficacy of chemical sterilization and storage conditions of gutta-percha cones. Int Endod J 2001;34:435-439. https://doi.org/10.1046/j.1365-2591.2001.00412.x
  5. Panuganti V, Vivek VJ, Jayashankara CM, Anilkumar S, Girish SA, Nanjundasett JK. Gutta-percha disinfection: a knowledge, attitude, and practice study among endodontic postgraduate students in India. Saudi Endod J 2016;6:127-130. https://doi.org/10.4103/1658-5984.189353
  6. Subha N, Prabhakar V, Koshy M, Abinaya K, Prabu M, Thangavelu L. Efficacy of peracetic acid in rapid disinfection of Resilon and gutta-percha cones compared with sodium hypochlorite, chlorhexidine, and povidone-iodine. J Endod 2013;39:1261-1264. https://doi.org/10.1016/j.joen.2013.06.022
  7. Chandrappa MM, Mundathodu N, Srinivasan R, Nasreen F, Kavitha P, Shetty A. Disinfection of gutta-percha cones using three reagents and their residual effects. J Conserv Dent 2014;17:571-574. https://doi.org/10.4103/0972-0707.144607
  8. Hamza MO, Gufran K, Baroudi K. Assessment of the potential of CFC (Calcium hydroxide Flagyl Ciprofloxacin) for the rapid disinfection of Resilon and gutta-percha. J Clin Diagn Res 2015;9:ZC40-ZC43.
  9. Senia ES, Marraro RV, Mitchell JL, Lewis AG, Thomas L. Rapid sterilization of gutta-percha cones with 5.25% sodium hypochlorite. J Endod 1975;1:136-140. https://doi.org/10.1016/S0099-2399(75)80098-7
  10. Short RD, Dorn SO, Kuttler S. The crystallization of sodium hypochlorite on gutta-percha cones after the rapid-sterilization technique: an SEM study. J Endod 2003;29:670-673. https://doi.org/10.1097/00004770-200310000-00015
  11. Pallotta RC, Ribeiro MS, de Lima Machado ME. Determination of the minimum inhibitory concentration of four medicaments used as intracanal medication. Aust Endod J 2007;33:107-111. https://doi.org/10.1111/j.1747-4477.2007.00095.x
  12. Nabeshima CK, Machado ME, Britto ML, Pallotta RC. Effectiveness of different chemical agents for disinfection of gutta-percha cones. Aust Endod J 2011;37:118-121.
  13. Gomes BP, Vianna ME, Matsumoto CU, Rossi Vde P, Zaia AA, Ferraz CC, Souza Filho FJ. Disinfection of gutta-percha cones with chlorhexidine and sodium hypochlorite. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005;100:512-517. https://doi.org/10.1016/j.tripleo.2004.10.002
  14. Pang NS, Jung IY, Bae KS, Baek SH, Lee WC, Kum KY. Effects of short-term chemical disinfection of gutta-percha cones: identification of affected microbes and alterations in surface texture and physical properties. J Endod 2007;33:594-598. https://doi.org/10.1016/j.joen.2007.01.019
  15. Kumar G, Jalaluddin M, Rout P, Mohanty R, Dileep CL. Emerging trends of herbal care in dentistry. J Clin Diagn Res 2013;7:1827-1829.
  16. Rajesvari R, Lakshmi T. Lemon grass oil for improvement of oral health. Dent Hypotheses 2013;4:115-117. https://doi.org/10.4103/2155-8213.122671
  17. Shenoi PR, Morey ES, Makade C, Gunwal MK, Wanmali SS. To evaluate the antimicrobial activity of herbal extracts and their efficacy in disinfecting gutta percha cones before obturation-an in vitro study. J Med Sci Clin Res 2014;2:2676-2684.
  18. Athiban PP, Borthakur BJ, Ganesan S, Swathika B. Evaluation of antimicrobial efficacy of Aloe vera and its effectiveness in decontaminating gutta percha cones. J Conserv Dent 2012;15:246-248. https://doi.org/10.4103/0972-0707.97949
  19. Falcao MA, Fianco AL, Lucas AM, Pereira MA, Torres FC, Vargas RM, Cassel E. Determination of antibacterial activity of vacuum distillation fractions of lemongrass essential oil. Phytochem Rev 2012;11:405-412. https://doi.org/10.1007/s11101-012-9255-3
  20. Garcia LS, Isenberg HD. Clinical microbiology procedures handbook - volume 1. 3rd ed. Washington, D.C.: ASM Press; 2010.
  21. Gomes BP, Pedroso JA, Jacinto RC, Vianna ME, Ferraz CC, Zaia AA, de Souza-Filho FJ. In vitro evaluation of the antimicrobial activity of five root canal sealers. Braz Dent J 2004;15:30-35. https://doi.org/10.1590/S0103-64402004000100006
  22. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006;32:93-98.
  23. Love RM. Enterococcus faecalis--a mechanism for its role in endodontic failure. Int Endod J 2001;34:399-405. https://doi.org/10.1046/j.1365-2591.2001.00437.x
  24. Waltimo TM, Haapasalo M, Zehnder M, Meyer J. Clinical aspects related to endodontic yeast infections. Endod Topics 2004;9:66-78. https://doi.org/10.1111/j.1601-1546.2004.00086.x
  25. Baumgartner JC, Siqueira JF Jr, Sedgley CM, Kishen A. Microbiology of endodontic disease. In: Ingle JI, Bakland LK, Baumgartner JC, editors. Ingle's endodontics.6th ed. Hamilton: BC Decker; 2008. p221-308.
  26. Street RA, Prinsloo G. Commercially important medicinal plants of South Africa: a review. J Chem 2013;2013:205048.
  27. Jaikaria A, Thakur S, Jayam C. Natural products used in dentistry - a review. Int J Oral Health Dent 2016;2:209-212.
  28. Saddiq AA, Khayyat SA. Chemical and antimicrobial studies of monoterpene: citral. Pestic Biochem Physiol 2010;98:89-93. https://doi.org/10.1016/j.pestbp.2010.05.004
  29. Silva CB, Guterres SS, Weisheimer V, Schapoval EE. Antifungal activity of the lemongrass oil and citral against Candida spp. Braz J Infect Dis 2008;12:63-66.
  30. Tyagi AK, Malik A. Liquid and vapour-phase antifungal activities of selected essential oils against Candida albicans: microscopic observations and chemical characterization of Cymbopogon citratus. BMC Complement Altern Med 2010;10:65. https://doi.org/10.1186/1472-6882-10-65
  31. Naik MI, Fomda BA, Jaykumar E, Bhat JA. On antibacterial activity of lemongrass (Cymbopogon citratus) oil against some selected pathogenic bacteria. Asian Pac J Trop Med 2010;3:535-538. https://doi.org/10.1016/S1995-7645(10)60129-0
  32. Suppakul P, Miltz J, Sonneveld K, Bigger SW. Antimicrobial properties of basil and its possible application in food packaging. J Agric Food Chem 2003;51:3197-3207. https://doi.org/10.1021/jf021038t
  33. Hossain MA, Kabir MJ, Salehuddin SM, Rahman SM, Das AK, Singha SK, Alam MK, Rahman A. Antibacterial properties of essential oils and methanol extracts of sweet basil Ocimum basilicum occurring in Bangladesh. Pharm Biol 2010;48:504-511. https://doi.org/10.3109/13880200903190977
  34. Elgayyar M, Draughon FA, Golden DA, Mount JR. Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. J Food Prot 2001;64:1019-1024. https://doi.org/10.4315/0362-028X-64.7.1019
  35. Hovijitra RS, Choonharuangdej S, Srithavaj T. Effect of essential oils prepared from Thai culinary herbs on sessile Candida albicans cultures. J Oral Sci 2016;58:365-371. https://doi.org/10.2334/josnusd.15-0736
  36. Burt S. Essential oils: their antibacterial properties and potential applications in foods--a review. Int J Food Microbiol 2004;94:223-253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
  37. Prabhakar J, Senthilkumar M, Priya MS, Mahalakshmi K, Sehgal PK, Sukumaran VG. Evaluation of antimicrobial efficacy of herbal alternatives (Triphala and green tea polyphenols), MTAD, and 5% sodium hypochlorite against Enterococcus faecalis biofilm formed on tooth substrate: an in vitro study. J Endod 2010;36:83-86.
  38. Shiva Rani SK, Saxena N. Udaysree . Antimicrobial activity of black pepper (Piper nigrum L). Glob J Pharmacol 2013;7:87-90.
  39. Roder E. The synergistic and individual anti-microbial impacts of green tea and ginger on common gastro-intestinal bacteria. Curr Med Chem J 2004;11:34-38.

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