Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Exercise induced upregulation of glutamate-cysteine ligase catalytic subunit and glutamate-cysteine ligase modifier subunit gene expression in Thoroughbred horses

  • Park, Jeong-Woong (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Choi, Jae-Young (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Hong, Seul A (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Kim, Nam Young (National Institute of Animal Science, Rural Development Administration) ;
  • Do, Kyoung-Tag (Department of Animal Biotehnology, Faculty of Biotechnology, Jeju National University) ;
  • Song, Ki-Duk (Department of Animal Biotechnology, Chonbuk National, University) ;
  • Cho, Byung-Wook (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University)
  • Received : 2016.10.08
  • Accepted : 2016.12.29
  • Published : 2017.05.01
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Abstract

Objective: This study was performed to reveal the molecular structure and expression patterns of horse glutamate-cysteine ligase catalytic subunit (GCLC) and glutamate-cysteine ligase modifier subunit (GCLM) genes whose products form glutamate cysteine ligase, which were identified as differentially expressed genes in the previous study. Methods: We performed bioinformatics analyses, and gene expression assay with quantitative polymerase chain reaction (qPCR) for horse GCLC and GCLM genes in muscle and blood leukocytes of Thoroughbred horses Results: Expression of GCLC showed the same pattern in both blood and muscle tissues after exercise. Expression of GCLC increased in the muscle and blood of Thoroughbreds, suggesting a tissue-specific regulatory mechanism for the expression of GCLC. In addition, expression of the GCLM gene increased after exercise in both the blood and muscle of Thoroughbreds. Conclusion: We established the expression patterns of GCLC and GCLM in the skeletal muscle and blood of Thoroughbred horses in response to exercise. Further study is now warranted to uncover the functional importance of these genes in exercise and recovery in racehorses.

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References

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