Asian-Australasian Journal of Animal Sciences

  • 제30권6호
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  • Pages.788-796
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  • 2017
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Silage preparation and fermentation quality of natural grasses treated with lactic acid bacteria and cellulase in meadow steppe and typical steppe

  • Hou, Meiling (Laboratory of Grassland Resources, Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University) ;
  • Gentu, Ge (Laboratory of Grassland Resources, Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University) ;
  • Liu, Tingyu (College of Agriculture, Inner Mongolia University of Nationalities) ;
  • Jia, Yushan (Laboratory of Grassland Resources, Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University) ;
  • Cai, Yimin (Institute of Livestock and Grassland Science, NARO)
  • 투고 : 2016.07.27
  • 심사 : 2016.09.20
  • 발행 : 2017.06.01
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초록

Objective: In order to improve fermentation quality of natural grasses, their silage preparation and fermentation quality in meadow steppe (MS) and typical steppe (TS) were studied. Methods: The small-scale silages and round bale silages of mixed natural grasses in both steppes were prepared using the commercial lactic acid bacteria (LAB) inoculants Chikuso-1 (CH, Lactobacillus plantarum) and cellulase enzyme (AC, Acremonium cellulase) as additives. Results: MS and TS contained 33 and 9 species of natural grasses, respectively. Stipa baicalensis in MS and Stipa grandi in TS were the dominant grasses with the highest dry matter (DM) yield. The crude protein (CP), neutral detergent fiber and water-soluble carbohydrate of the mixed natural grasses in both steppes were 8.02% to 9.03%, 66.75% to 69.47%, and 2.02% to 2.20% on a DM basis, respectively. All silages treated with LAB and cellulase were well preserved with lower pH, butyric acid and ammonia-N content, and higher lactic acid and CP content than those of control in four kinds of silages. Compared with CH- or AC-treated silages, the CH+ AC-treated silages had higher lactic acid content. Conclusion: The results confirmed that combination with LAB and cellulase may result in beneficial effects by improving the natural grass silage fermentation in both grasslands.

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참고문헌

  1. Zhang J, Guo G, Chen L, et al. Effect of applying lactic acid bacteria and propionic acid on fermentation quality and aerobic stability of oats-common vetch mixed silage on the Tibetan plateau. J Anim Sci 2015;86:595-602. https://doi.org/10.1111/asj.12340
  2. Scarbrough DA, Coblentz WK, Coffey KP, et al. Effects of summer management and fall harvest date on ruminal in situ degradation of crude protein in stockpiled bermudagrass. Anim Feed Sci Technol 2002;96:119-33. https://doi.org/10.1016/S0377-8401(01)00349-2
  3. Kaiser AG, Curll ML. Improving the efficiency of forage conservation from pastures. In Wheeler JL, Pearson CJ, Robards GE, editors, Temperate pastures: their production, use and management. Melbourne, Australia; CSIRO Publishing Publishing, 1987. p. 397-411.
  4. King C, McEniry J, O’Kiely P. A note on the fermentation characteristics of red clover silage in response to advancing stage of maturity in the primary growth. Irish J Agric Food Res 2012;51:79-84.
  5. Filya I, Sucu E. The effects of lactic acid bacteria on the fermentation, aerobic stability and nutritive value of maize silage. Grass Forage Sci 2010;65:446-55. https://doi.org/10.1111/j.1365-2494.2010.00763.x
  6. Li M, Zi X, Zhou H, Hou G, Cai Y. Effects of sucrose, glucose, molasses and cellulase on fermentation quality and in vitro gas production of king grass silage. Anim Feed Sci Technol 2014;197:206-12. https://doi.org/10.1016/j.anifeedsci.2014.06.016
  7. Weinberg Z, Ashbell G, Hen Y, Azrieli A. The effect of cellulase and heicellulase plus pectinase on the aerobic stability and fiber analysis of peas and wheat silages. Anim Feed Sci Technol 1995;55:287-93. https://doi.org/10.1016/0377-8401(95)00785-L
  8. Muck RE. Initial bacteria numbers on lucerne prior to ensiling. Grass Forage Sci 1989;44:19-25. https://doi.org/10.1111/j.1365-2494.1989.tb01905.x
  9. Colombatto D, Mould FL, Bhat MK, et al. Influence of fibrolytic enzymes on the hydrolysis and fermentation of pure celluloseand xylan by mixed ruminal microorganisms in vitro. J Anim Sci 2003;81: 1040-50. https://doi.org/10.2527/2003.8141040x
  10. Liu G, Xie X, Ye D, et al. Plant functional diversity and species diversity in the Mongolian steppe. PLoS ONE 2013;8:e77565. https://doi.org/10.1371/journal.pone.0077565
  11. Munson SM, Lauenroth WK. Plant Community recovery following restoration in semiarid grasslands. Res Ecol 2012;20:656-63. https://doi.org/10.1111/j.1526-100X.2011.00808.x
  12. Wang Z, Zhang Q, Xin X, et al. Response of the annual biomass production of a typical steppe plant community to precipitation fluctuations. Rangeland J 2014;36:527-36.
  13. Cai Y. Analysis method for silage. In: Japanase Society of Grassland Science, editors. Field and laboratory methods for grassland science. Tokyo, Japan: Thsho Printing Co., Ltd.; 2004. p. 279-82.
  14. Cai Y, Benno Y, Ogawa M, Kumai S. Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage. J Dairy Sci 1999;82:520-6. https://doi.org/10.3168/jds.S0022-0302(99)75263-X
  15. Horwitz W, Latimer Jr. GW. AOAC International. Official methods of analysis of AOAC International. 18th ed. Gaithersburg, MD: AOAC International; 2005.
  16. Soest VJP, Robertson JB, Lewis BA. Methods fro dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  17. Thomas T. An automated procedure for the determination of soluble carbohydrates in herbage. J Sci Food Agric 1997;28:639-42.
  18. Saidin S, Kadir MRA, Sulaiman E, Kasim, NHA. Effects of different implant-abutment connections on micromotion and stress distribution: Prediction of microgap formation. J Dent 2012;40:467-74. https://doi.org/10.1016/j.jdent.2012.02.009
  19. Wu X, Liu G, Butterbach-Bahl K, et al. Effects of land cover and soil properties on denitrification potential in soils of two semi-arid grasslands in Inner Mongolia, China. J Arid Environ 2013;92:98-101. https://doi.org/10.1016/j.jaridenv.2013.02.003
  20. Zhou, TR. Analyze of bale silage manufactur technique and quality control of natural grassland herbage [master's thesis]. Hohhot, China: Inner Mongolia Agriculture University; 2015.
  21. Buheaosi. Grassland resources and evaluation in Ewenke Autonomous Banner, 2 th ed. Beijing: China Agriculture Press; 2013.
  22. Li X, Li G, Wang H, Wang H, Yu J. Influence of meadow changes on net primary productivity: a case study in a typical steppe area of XilinGol of Inner Mongolia in China. Geosciences J 2015;19:561-73. https://doi.org/10.1007/s12303-014-0057-z
  23. Li F, Zeng Y, Luo JH, Ma RH, Wu BF. Modeling grassland aboveground biomass using a pure vegetation index. Ecol Indic 2016;62:279-88. https://doi.org/10.1016/j.ecolind.2015.11.005
  24. Zhang Q, Wu B, Nishino N, Wang X, Yu Z. Fermentation and microbial population dynamics during the ensiling of native grass and subsequent exposure to air. J Anim Sci 2016;87:389-97. https://doi.org/10.1111/asj.12427
  25. Zhu T. Grassland resources and development of grassland agriculture in temperate China. Rangelands 1998;10:124-7.
  26. Goomaral A, Undarmaa J, Matsumoto T, Yamato M. Effect of plant species on communities of arbuscular mycorrhizal fungi in the Mongolian steppe. Mycoscience 2013;54:362-7. https://doi.org/10.1016/j.myc.2012.12.005
  27. Jin YX, Yang XC, Qiu JJ, et al. Remote sensing-based biomass estimation and its spatio-temporal variations in temperate grassland, Northern China. Remote Sens 2014;6:1496-513. https://doi.org/10.3390/rs6021496
  28. Cherney DJR, Alessi MA, Cherney JH. Influence of grass species and sample preparation on ensiling characteristics. Crop Sci 2006;46: 256-63. https://doi.org/10.2135/cropsci2005.04-0032
  29. Borreani G, Giaccone D, Mimosi A, Tabacco E. Comparison of hay and haylage from permanent Alpine meadows in winter dairy cow diets. J Dairy Sci 2007;90:5643-50. https://doi.org/10.3168/jds.2007-0134
  30. McEniry J, Forristal PD, O'Kiely P. Factors influencing the conservation characteristics of baled and precision-chop grass silages. Irish J Agric Food Res 2011;50:175-88.
  31. Weinberg ZG, Ashbell G, Azrieli A, Brukental I. Ensiling peas, ryegrass and wheat with additives of lactic acid bacteria (LAB) and cell wall degrading enzymes. Grass Forage Sci 1993;48:70-8. https://doi.org/10.1111/j.1365-2494.1993.tb01838.x
  32. McDonald P, Henderson AR. Determination of water-soluble carbohydrates in grass. J Sci Food Agric 1964;15:395-8. https://doi.org/10.1002/jsfa.2740150609
  33. Amer S, Hassanat F, Berthiaume R, Seguin P, Mustafa AF. Effects of water soluble carbohydrate content on ensiling characteristics, chemical composition and in vitro gas production of forage millet and forage sorghum silages. Anim Feed Sci Technol 2012;177:23-9. https://doi.org/10.1016/j.anifeedsci.2012.07.024
  34. Cao Y, Takahashi T, Horiguchi K-i, Yoshida N, Cai Y. Methane emissions from sheep fed fermented or non-fermented total mixed ration containing whole-crop rice and rice bran. Anim Feed Sci Technol 2010;157:72-8. https://doi.org/10.1016/j.anifeedsci.2010.02.004
  35. Sun ZH, Liu SM, Tayo GO, et al. Effects of cellulase or lactic acid bacteria on silage fermentation and in gas production of several morphological fractions of maize stover. Anim Feed Sci Technol 2009;152:219-31. https://doi.org/10.1016/j.anifeedsci.2009.04.013
  36. Napasirth V, Napasirth P, Sulinthone T, Phommachanh K, Cai YM. Microbial population, chemical composition and silage fermentation of cassava residues. J Anim Sci 2015;86:842-8.
  37. Li DX, Ni KK, Pang HL, et al. Identification and antimicrobial activity detection of lactic acid bacteria isolated from corn stover silage. Asian-Australas J Anim Sci 2015;28:620-31. https://doi.org/10.5713/ajas.14.0439
  38. Bureenok S, Yuangklang C, Vasupen K, Schonewille JT, Kawamoto Y. The effects of additives in napier grass silages on chemical composition, feed intake, nutrient digestibility and rumen fermentation. Asian-Australas J Anim Sci 2012;25:1248-54. https://doi.org/10.5713/ajas.2012.12081
  39. McEniry J, King C, O'Kiely P. Silage fermentation characteristics of three common grassland species in response to advancing stage of maturity and additive application. Grass Forage Sci 2014;69:393-404. https://doi.org/10.1111/gfs.12038
  40. Cao LM, Goto M, Karita S, et al. Effect of fermented juice of epiphytic lactic acid bacteria on the fermentation quality of alfalfa (Medicago sativa L.) silage and its energy and nitrogen utilization by dry cows. Grass Forage Sci 2002;48:227-35.
  41. Wang J, Wang JQ, Zhou H, Feng T. Effects of addition of previously fermented juice prepared from alfalfa on fermentation quality and protein degradation of alfalfa silage. Anim Feed Sci Technol 2009; 151:280-90. https://doi.org/10.1016/j.anifeedsci.2009.03.001
  42. Borreani G, Tabacco E. Improving corn silage quality in the top layer of farm bunker silos through the use of a next-generation barrier film with high impermeability to oxygen. J Dairy Sci 2014;97:2415-26. https://doi.org/10.3168/jds.2013-7632

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  7. Microbial Community and Fermentation Characteristics of Native Grass Prepared Without or With Isolated Lactic Acid Bacteria on the Mongolian Plateau vol.12, pp.None, 2017, https://doi.org/10.3389/fmicb.2021.731770