Current Optics and Photonics

Optical Society of Korea (한국광학회)
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Demonstration of the Usefulness of Optical Coherence Tomography in Imaging a Mouse Tail Model of Lymphedema

  • Kim, Hui Dong (Department of Physical Medicine and Rehabilitation, College of Medicine, Kosin University) ;
  • Kim, Dong Kyu (Department of Physical Medicine and Rehabilitation, College of Medicine, Kosin University) ;
  • Chae, Yu-Gyeong (Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology, Pukyong National University) ;
  • Park, Seok Gyo (Department of Physical Medicine and Rehabilitation, College of Medicine, Kosin University) ;
  • Kim, Ghi Chan (Department of Physical Medicine and Rehabilitation, College of Medicine, Kosin University) ;
  • Jeong, Ho Joong (Department of Physical Medicine and Rehabilitation, College of Medicine, Kosin University) ;
  • Sim, Young-Joo (Department of Physical Medicine and Rehabilitation, College of Medicine, Kosin University) ;
  • Ahn, Yeh-Chan (Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology, Pukyong National University)
  • Received : 2016.09.07
  • Accepted : 2017.01.05
  • Published : 2017.04.25
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Abstract

To investigate the usefulness of optical coherence tomography (OCT) for imaging lymphedema, we directly compared it to other histological methods in a mouse model of lymphedema. We performed detailed imaging of the lymphedema lesion on a mouse tail. We imaged the mouse tail in vivo with OCT and created histopathological samples. We constructed a spectrometer-based OCT system using a fiber-optic Michelson interferometer. The light was directed to 50:50 couplers that split the light into reference and sample arms. Backscattered light from a reference mirror and the sample produced an interference fringe. An OCT image of the lymphedema model revealed an inflammatory reaction of the skin that was accompanied by edema, leading to an increase in the light attenuation in the dermal and subcutaneous layers. Similar to OCT image findings, histological biopsy showed an inflammatory response that involved edema, increased neutrophils in epidermis and subdermis, and lymphatic microvascular dilatation. Furthermore, the lymphedema model showed an increase in thickness of the dermis in both diagnostic studies. In the mouse tail model of lymphedema, OCT imaging showed very similar results to other histological examinations. OCT provides a quick and useful diagnostic imaging technique for lymphedema and is a valuable addition or complement to other noninvasive imaging tools.

Keywords

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