Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Quantitative Analysis of Chrysin and Pinocembrin in Korean Propolis

국산 프로폴리스 내 Chrysin과 Pinocembrin의 정량분석

  • Kim, Se Gun (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hong, In Pyo (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Woo, Soon Ok (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Jang, Hye Ri (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Han, Sang Mi (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
  • 김세건 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 홍인표 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 우순옥 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 장혜리 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 한상미 (농촌진흥청 국립농업과학원 농업생물부)
  • Received : 2017.01.05
  • Accepted : 2017.03.03
  • Published : 2017.03.31
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Abstract

In the present study, we carried out quantitative analysis of chrysin and pinocembrin in Korean propolis by ultra performance liquid chromatography (UPLC) equipped with diode array detector. The separation was done using BEH C18 ($2.1{\times}50mm$, $1.7{\mu}m$) column with a mobile phase consisting of MeCN and 0.1% $H_3PO_4$ at 280 nm. The chromatographic method was validated for specificity, limit of detection, limit of quantification, linearity, precision, and accuracy. A quantitative analysis exhibited that the contents of the two compounds in Korean propolis collected from 8 inland areas except Jeju-do ranged from 3.1-46.0 mg/g. These results will be valuable as basic data for standardization of Korean propolis.

Keywords

References

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