인천지역 유통 생약 중 Ochratoxin A 오염실태 조사

A Survey on Ochratoxin a Contamination of Commercial Medicinal Herbs in Incheon

  • 임수선 (인천광역시 보건환경연구원, 약품분석과) ;
  • 주광식 (인천광역시 보건환경연구원, 약품분석과) ;
  • 최은정 (인천광역시 보건환경연구원, 약품분석과) ;
  • 이수연 (인천광역시 보건환경연구원, 약품분석과) ;
  • 임윤선 (인천광역시 보건환경연구원, 약품분석과) ;
  • 엄선아 (인천광역시 보건환경연구원, 약품분석과) ;
  • 조남규 (인천광역시 보건환경연구원, 약품분석과) ;
  • 이성모 (인천광역시 보건환경연구원, 약품분석과)
  • Lim, Soo-sun (Pharmaceutical Analysis Division, Incheon Metropolitan Government Research Institute of Public Health and Environment) ;
  • Joo, Gwang-sig (Pharmaceutical Analysis Division, Incheon Metropolitan Government Research Institute of Public Health and Environment) ;
  • Choi, Eun-Jeong (Pharmaceutical Analysis Division, Incheon Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Soo-Yeon (Pharmaceutical Analysis Division, Incheon Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lim, Yun-sun (Pharmaceutical Analysis Division, Incheon Metropolitan Government Research Institute of Public Health and Environment) ;
  • Eum, Sun-Ah (Pharmaceutical Analysis Division, Incheon Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jo, Nam-Kyu (Pharmaceutical Analysis Division, Incheon Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Sung-Mo (Pharmaceutical Analysis Division, Incheon Metropolitan Government Research Institute of Public Health and Environment)
  • 투고 : 2016.11.19
  • 심사 : 2017.02.06
  • 발행 : 2017.03.31

초록

The aim of this study was to screen the contamination by ochratoxin A of mycotoxins in various medicinal herbs. We conducted a survey of ochratoxin A in medicinal herb on the retail market in Incheon in 2016. 116 medicinal herb samples were evaluated for the ochratoxin A contamination. They were analyzed for ochratoxin A using immunoaffinity column and high-performance liquid chromatography(HPLC)-fluorescence detection and the positive samples were confirmed using HPLC-tandem mass spectrometry. Ochratoxin A was detected in 4 medicinal herb samples; the concentrations of ochratoxin A were containing between 20.11 and $372.90{\mu}g/kg$. This study shows that in general, this kind of commodity may be contaminated by mycotoxins. Also this contamination is not limited to only aflatoxin of mycotoxins.

키워드

참고문헌

  1. Santos, L., Marin, S., Sanchis, V. and Ramos, A. J. (2009) Screening of mycotoxin multicontamination in medicinal and aromatic herbs sampled in spain. J. Sci. Food Agric. 89: 1802-1807. https://doi.org/10.1002/jsfa.3647
  2. O'Brien, E., Heussner, A. H. and Dietrich, D. R. (2001) Species-, sex-, and cell type-specific effects of ochratoxin A and B. TOXICOL. Sci. 63: 254-264.
  3. Ciegler, A. (1972) Bioproduction of ochratoxin A and penicillic acid by members of the aspergillus ochraceus group. Can. J. Microbiol. 18: 631-636. https://doi.org/10.1139/m72-100
  4. Moss, O. M. (1996) Mode of formation of ochratoxin A. Food Additives and Contaminants 13: 5-9. https://doi.org/10.1080/02652039609374376
  5. Pitt, J. I. (1987) Penicillium viridicatum, penicillium verrucosum, and production of ochratoxin A. Appl. Environ. Microbiol. 53: 266-269.
  6. Kang, S. J., Park, B. J., Lee, J. O., Kang, J. S. and Chung, D. H. (1998) Screening of ochratoxin A producing fungi from greenhouse horticulture. Korean J. Food Sci. Technol. 30: 1415-1419.
  7. Lee, H. G., Hwang, Y. H., Kim, M. J., Kim, M. G., Lee, S. E. and Lee, H. S. (2002) Toxicity and metabolism of mycotoxins occurring in foods and feeds. J. Korean Soc. Agric. Chem. Biotechnol. 45: 1-10.
  8. Park, J. W., Chung, S. H. and Kim, Y. B. (2005) Ochratoxin A in Korean food commodities:Occurrence and safety evaluation. J. Agric. Food Chem. 53: 4637-4642. https://doi.org/10.1021/jf050227j
  9. Park, J. W. (2006) Revision of the analysis of makgeolli for ochratoxin A. Korean J. Food Sci. Technol. 38: 140-142.
  10. Kim, D. H., Jang, H. S., Kim, Y. M. and Ahn, J. S. (2009) Survey for contamination and study for reduction of ochratoxin A and aflatoxin in red pepper. J. Food Hyg. Safety 24: 299-306.
  11. Sugita-Konishi, Y., Nakajima, M., Tabata, S., Ishikuro, E., Tanaka T., Norizuki, H., Itoh, Y., Aoyama, K., Fujita, K., Kai, S. and Ku magai, S. (2006) Occurrence of aflatoxins, ochratoxin A, and fumonisins in retail foods in japan. Journal of Food Protection$^{(R)}$ 69: 1365-1370. https://doi.org/10.4315/0362-028X-69.6.1365
  12. I. A. R. C. (2016) Monographs on the evaluation of carcinogenic risks to humans. 1-117. available from: http//monographs.iarc.fr/ENG/Classification/latest_classif.php accessed Dec. 23.
  13. Lee, J. G., Kang, Y. W., Jeong, J. H., Noh, M. J., Ahn, E. S., Lee, K. H. and Kim, M. H. (2012) Monitoring of ochratoxin in alcoholic beverages. Korean J. Food Sci. Technol. 44: 235-239. https://doi.org/10.9721/KJFST.2012.44.2.235
  14. Kang, K. J., Kim, H. J., Lee, Y. G., Jung, K. H., Han, S. B., Park, S. H. and Oh, H. Y. (2010) Administration of mycotoxins in food in korea. J. Food Hyg. Safety 25: 281-288.
  15. European commission (2010) Commission regulation No. 105/2010 of 5 february 2010 amending regulation(EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards ochratoxin A, Official journal of the european union No. L35/7 of february 6th, 2010.
  16. 송정언 (2011) 한약재에서 탕액으로의 ochratoxin A의 이행율 평가. 경상대 석사학위논문.
  17. 식품의약품안전처 (2014) 대한민국약전.
  18. Paek, O. J., Park, S. Y., Park, K. H., Kim, S. H., Suh, S. J. and Yoon, H. J. (2016) Simultaneous determination of aflatoxins and ochratoxin A in pork by LC-MS/MS. J. Food Hyg. Safety 31: 194-200. https://doi.org/10.13103/JFHS.2016.31.3.194
  19. Procedual Manual 21th ed. (2013) Codex alimentarius commission, joint WHO/FAO food standard programe, rome.
  20. Park, J. W., Yoo, M. S., Kuk, J. H., Ji, Y. A. and Lee, J.H. (2013) Simult-aneous determination and monitoring of aflatoxin and ochratoxin A in Food. J. Food Hyg. Safety 28: 75-82. https://doi.org/10.13103/JFHS.2013.28.1.075
  21. Hierro, J. M. H., Garcia Villanova, R. J., Torrero, P. R. and Fonseca, I. M. T. (2008) Aflatoxins ochratoxin A in red paprika for retail sale in spain: Occurrence and evaluation of a simultaneous analytical method. J. Agric. Food Chem. 56: 751-756. https://doi.org/10.1021/jf073002c