Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Antioxidative Activity and Tyrosinase Inhibition Effect of Fractions from the Extract of Inonotus obliquus

차가버섯 용매별 분획물의 항산화 활성과 Tyrosinase 저해 활성

  • Im, Do-Youn (Division of Liberal Arts and Teacher Training, Kwangju Women's University) ;
  • Lee, Kyoung-In (Bio-center, Dongshin University)
  • 임도연 (광주여자대학교 교양교직과정부) ;
  • 이경인 (동신대학교 바이오센터)
  • Received : 2016.11.20
  • Accepted : 2017.02.06
  • Published : 2017.03.31
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Abstract

This study was conducted to investigate the antioxidative activities and tyrosinase inhibition effects of fractions from the distilled water extract of Inonotus obliquus. Moreover, GC-MS based analysis with trimethylsilyl (TMS) derivatization was carried out for active compound in the extract of I. obliquus. In DPPH radical scavenging ability, $SC_{50}$ values of the ethyl acetate fraction was 0.393 mg/ml as a result of the most effective than other fractions. Meanwhile, aqueous fraction showed higher effect in tyrosinase inhibitoty activity. In GC-MS based analysis with TMS derivatization, 7 compounds including syringic acid, vanillic acid and protocatechuic acid were observed in ethyl acetate fraction, and oxalic acid is the main compound in aqueous fraction. As a result, it was confirmed that oxalic acid in aqueous fraction from the distilled water extract of I. obliquus was a compound showing tyrosinase inhibition effect.

Keywords

References

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