Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Anti-inflammatory Activity of Essential Oil Extracted from Chamaecyparis obtusa (Sieb. et Zucc.) Wood

편백 목부 정유의 항염증 효과 평가

  • Yang, Jiyoon (Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science) ;
  • Ahn, Changhwan (College of Veterinary Medicine, Chungbuk National University) ;
  • Jeung, Eui-Bae (College of Veterinary Medicine, Chungbuk National University) ;
  • Choi, Won-Sil (National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Kim, Jae-Woo (Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science) ;
  • Park, Mi-Jin (Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science)
  • 양지윤 (국립산림과학원 임산공학부 화학미생물과) ;
  • 안창환 (충북대학교 수의대학교) ;
  • 정의배 (충북대학교 수의대학교) ;
  • 최원실 (서울대 NICEM) ;
  • 김재우 (국립산림과학원 임산공학부 화학미생물과) ;
  • 박미진 (국립산림과학원 임산공학부 화학미생물과)
  • Received : 2016.11.19
  • Accepted : 2017.02.06
  • Published : 2017.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to determine the anti-inflammatory effect of essential oil extracted from the wood of Chamaecyparis obtusa Sieb. et Zucc. Endl. (Cupressaceae). The essential oil was extracted from the wood of C. obtusa by hydrodistillation method, and conducted the analysis on the chemical composition of the extracted C. obtusa wood oil through GC-MS. The major constituents of the oil were found to be: ${\alpha}-pinene$ (11.4%), cadinene (5.4%), ${\delta}-cadiene$ (9.0%), ${\tau}-muurolol$ (22.2%), ${\alpha}-cadinol$ (20.8%) etc. We attempted to identify the anti-inflammatory activities of the oil when it is injected in the lipopolysaccharide (LPS)-stimulated RBL-2H3 cells, along with its effects on the secretion of interleukin-4 (IL-4), interleukin-13 (IL-13), ${\beta}-hexosaminidase$. According to the cell viability analysis conducted by MTT assay, the oil in $10^{-7}{\sim}10^{-5}%$concentration showed no effect on the cell viability. After RBL-2H3 cells treated by LPS stimulation were exposed to $10^{-7}%$ concentration of C. obtusa wood oil, the expression levels of IL-4, IL-13 within the cell were observed to remarkably decrease. Also, it was attenuated the release of ${\beta}-hexosaminidase$ from mast cells to a significantly meaningful level. These results suggest that C. obtusa wood oil exerts the anti-inflammatory effect, by regulating the expression of inflammatory cytokines, which is a valuable feature to be highly utilized as the functional materials in the future.

Keywords

References

  1. Hong, C. U., Kim, C. S., Kim, N. G. and Kim, Y. H. (2001) Composition of essential oils from the leaves and the fruits of Chamaecyparis obtusa and Chamaecyparis pisifera. J. Korean Soc. Agric. Chem. Biotechnol. 44: 116-121.
  2. Kim, K. H., Kim, B. C., Shin, C. G., Jeong, S. I., Kim, H. J. and Ju, Y. S. (2004) Susceptibility of oral bacteria to essential oil of Artemisia capillaris Thunb. J. Korean Orient. Med. Soc. 25: 121-128.
  3. Park, M. J., Lee, S. M., Gwak, K. S., Jeung, E. B., Chang, J. W. and Choi, I. G. (2005) Investigation of active antifungal compounds of essential oil from Chamaecyparis obstusa against dermatophytes, Microsporum canis and Trichophyton mentagrophytes. J. Korean Wood Sci. Technol. 33: 72-78.
  4. Lee, S. S., Kang, H. Y. and Choi, I. G. (2002) Studies on biological activities of woody essential oils (I). J. Korean Wood Sci. Technol. 30: 48-55.
  5. Park, Y., Jung, S. M., Yoo, S. A., Kim, W. U., Cho, C. S., Park, B. J., Woo, J. M. and Yoon, C. H. (2015) Antinociceptive and anti-inflammatory effects of essential oil extracted from Chamaecyparis obtusa in mice. Int. Immunopharmacol. 29: 320-325. https://doi.org/10.1016/j.intimp.2015.10.034
  6. Park, M. J., Choi, W. S., Min, B. C., Kim, H. Y., Kang, H. Y. and Choi, I. G. (2008) Antioxidant activities of essential oils from Chamaecyparis obtusa. J. Korean Wood Sci. Technol. 36: 159-167.
  7. Koyama, S., Tanaka, S., Yamaguchi, Y. and Motoyoshya, J. (1997) Apoptosis-like (Possible Quantum Thermodynamic) cell death induced by Yoshixol and wood oil of Chamaecyparis obtusa (Kiso-Hinoki) on HeLa cell. Gen. Pharmacol. 28: 805-811. https://doi.org/10.1016/S0306-3623(97)85608-X
  8. Mishra, A. K. and Dubey N. K. (1994) Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Appl. Environ. Microbiol. 60: 1101-1105.
  9. Price, S., L. Price, and S. Pe'noel D. (1995) Aromatherapy for health professionals 1st ed, 133-167, Chrchill Livingstone, New York.
  10. Lim, G. S., Kim, R., Cho, H., Moon, Y. S. and Choi, C. N. (2013) Comparison of volatile compounds of Chamaecyparis obtusa essential oil and its application on the improvement of atopic dermatitis. KSBB. J. 28: 115-122. https://doi.org/10.7841/ksbbj.2013.28.2.115
  11. Kim, H. G., Lee, J. H., Park, S. J., Kim, J. Y., Kim, B. H. and Park, D. I. (2008) Anti-allergic effect of Bopyeoyangyeongjun. Korean. J. Orient. Physiol. Pathol. 22: 660-665.
  12. Kim, H. S., Park, J. H., Kim, H. K., Kim, J. H., Lee, B., Min, J. H., Kim, E. Y., Jung, H. S., Lee, H. S. and Sohn, Y. J. (2014) Effects of ethanol extract of Liriope platyphylla on allergic inflammation. J. Physiol. & Pathol. Korean. Med. 28: 512-519.
  13. Lee, J. H., Kim, H. G., Shin, W. J., Kim, J. Y. and Park, D. I. (2009) Anti-allergic effect of Bopyeoyangyeong-jun to cytokines and transcription. Korean. J. Orient. Physiol. Pathol. 23: 127-134.
  14. Brown, J. M., Wilson, T. M. and Metcalfe, D. D. (2008) The mast cell and allergic diseases: role in pathogenesis and implications for therapy. Clin. Exp. Allergy 38: 4-18.
  15. Parham, P. (2011) Immunology, 351-352, Life Science, Seoul.
  16. Kim, Y. (2005) Basic Pathology, 138-140, 438-439, Hyunmoonsa, Seoul.
  17. Hack, C. E., Aarden, L. A. and Thus, L. G. (1997) Role of cytokines in Sepsis. Adv. Immunol. 66: 101-195.
  18. Georas, S. N., Guo, J., Fanis, D. and Casolaro, V. (2005) Thelper cell type-2 regulation in allergic disease. Eur. Respir. J. 26: 1119-1137. https://doi.org/10.1183/09031936.05.00006005
  19. Sohn, E. H., Jang, S. A., Joo, H., Park, S., Kang, S. C., Lee, C. H. and Kim, S. Y. (2011) Anti-allergic and anti-inflammatory effects of butanol extract from Arctium lappa L. Clin. Mol. Allergy 9: 1-11. https://doi.org/10.1186/1476-7961-9-1
  20. Boguniewicz, M. and Leung, D. Y. (2011) Atopic dermatitis: a disease of altered skin barrier and immune dysregulation. Immunol. Rev. 242: 233-246. https://doi.org/10.1111/j.1600-065X.2011.01027.x
  21. Toru, H., Pawankar, R., Ra, C., Yata, J. and Nakahata, T. (1998) Human mast cells produce IL-13 by high-affinity IgE receptor cross-linking: enhanced IL-13 production by IL-4-primed human mast cells. J. Allergy Clin. Immunol. 102: 491-502. https://doi.org/10.1016/S0091-6749(98)70140-X
  22. Metz, M., Grimbaldeston, M. A., Nakae, S., Piliponsky, A. M., Tsai, M. and Galli, S. J. (2007) Mast cells in the promotion and limitation of chronic inflammation. Immunol. Rev. 217: 304-328. https://doi.org/10.1111/j.1600-065X.2007.00520.x
  23. Kim, S. H., Park, H. H., Lee, S., Jun, C. D., Choi, B. J., Kim, S. Y., Kim, S. H., Kim, D. K., Park, J. S., Chae, B. S. and Shin, T. Y. (2005) The anti-anaphylactic effect of the gall of Rhus javanica is mediated through inhibition of histamine release and inflammatory cytokine secretion. Int. Immunopharmacol. 5: 1820-1829. https://doi.org/10.1016/j.intimp.2005.06.007
  24. Sode, K., Karube, I., Araki, R. and Mikami, Y. (1989) Microbial conversion of ${\beta}$-ionone by immobilized Aspergillus niger in the presence of an organic solvent. Biotechnol. Bioeng. 33: 1191-1195. https://doi.org/10.1002/bit.260330915
  25. Chang, S. T., Chen, P. F., Wang, S. Y. and Wu, H. H. (2001) Antimite activity of essential oils and their constituents from Taiwania cryptomerioides. J. Med. Entomol. 38: 455-457. https://doi.org/10.1603/0022-2585-38.3.455
  26. Kim, J. W., Choi, W. S., Lee, S. S. and Park, M. J. (2015) Comparative study on the composition of essential oil by supercritical carbon dioxide extraction and hydro-distillation from Chamaecyparis obtusa leaves. J. Korean Wood Sci. Technol. 43: 494-503. https://doi.org/10.5658/WOOD.2015.43.4.494
  27. Chien, T. C., Lo, S. F. and Ho, C. L. (2014) Chemical composition and anti-inflammatory activity of Chamaecyparis obtusa f. formosana wood essential oil from Taiwan. Nat. Prod. Commun. 9: 723-726.
  28. Yang, H., Jung, E. M., Ahn, C., Lee, G. S., Lee, S. Y., Kim, S. H., Choi, I. G., Park, M. J., Lee, S. S., Choi, D. H. and Jeung, E. B. (2015) Elemol from Chamaecyparis obtusa ameliorates 2, 4-dinitrochlorobenzene-induced atopic dermatitis. Int. J. Mol. Med. 36: 463-472. https://doi.org/10.3892/ijmm.2015.2228
  29. Macdonald, A. J., Haig, D. M., Bazin, H., Mcguigan, A. C., Moqbel R. and Miller H. R. P. (1989) IgE-mediated release of rat mast cell protease II, ${\beta}$-hexosaminidase and leukotriene $C_4$ from cultured bone marrow-derived rat mast cells. Immunology 67: 414-418.
  30. Choi, I., Kim, K., Kim, Y., Park, M. J., Lee, Y. S. and Jeoung, D. (2006) Fractions of Chamaecyparis obtusa display antiallergic effect in RBL-2H3 cells. J. Microbiol. Biotechnol. 16: 1747-1752.