Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Optimization of Fungal Enzyme Production by Trichoderma harzianum KUC1716 through Surfactant-Induced Morphological Changes

  • Lee, Hanbyul (Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University) ;
  • Lee, Young Min (Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University) ;
  • Heo, Young Mok (Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University) ;
  • Hong, Joo-Hyun (Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University) ;
  • Jang, Seokyoon (Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University) ;
  • Ahn, Byoung Jun (Division of Wood Chemistry and Microbiology, National Institute of Forest Science) ;
  • Lee, Sung-Suk (Division of Wood Chemistry and Microbiology, National Institute of Forest Science) ;
  • Kim, Jae-Jin (Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University)
  • Received : 2016.07.29
  • Accepted : 2017.01.04
  • Published : 2017.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The morphological optimization of Trichoderma harzianum was carried out using several surfactants to achieve increased cellulase production. Addition of the surfactants to the culture medium successfully modified the fungal morphology from an aggregated form to a dispersed form. Optimization of the fungal morphology increased cellulase activity up to 177%. The morphologically optimized conditions enhanced the accessibility of the fungus to substrates and thus promoted cellulase production.

Keywords

References

  1. Lynd LR, Weimer PJ, Van Zyl WH, Pretorius IS. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev 2002;66:506-77. https://doi.org/10.1128/MMBR.66.3.506-577.2002
  2. Lee YM, Lee H, Kim JS, Lee J, Ahn BJ, Kim GH, Kim JJ. Optimization of medium components for ${\beta}$-glucosidase production in Schizophyllum commune KUC9397 and enzymatic hydrolysis of lignocellulosic biomass. BioResources 2014;9: 4358-68.
  3. Deswal D, Khasa YP, Kuhad RC. Optimization of cellulase production by a brown rot fungus Fomitopsis sp. RCK2010 under solid state fermentation. Bioresour Technol 2011;102: 6065-72. https://doi.org/10.1016/j.biortech.2011.03.032
  4. Posch AE, Spadiut O, Herwig C. A novel method for fast and statistically verified morphological characterization of filamentous fungi. Fungal Genet Biol 2012;49:499-510. https://doi.org/10.1016/j.fgb.2012.05.003
  5. Johansen CL, Coolen L, Hunik JH. Influence of morphology on product formation in Aspergillus awamori during submerged fermentations. Biotechnol Prog 1998;14:233-40. https://doi.org/10.1021/bp980014x
  6. Domingues FC, Queiroz JA, Cabral JM, Fonseca LP. The influence of culture conditions on mycelial structure and cellulase production by Trichoderma reesei Rut C-30. Enzyme Microb Technol 2000;26:394-401. https://doi.org/10.1016/S0141-0229(99)00166-0
  7. Ahamed A, Vermette P. Effect of culture medium composition on Trichoderma reesei’s morphology and cellulase production. Bioresour Technol 2009;100:5979-87. https://doi.org/10.1016/j.biortech.2009.02.070
  8. Bhargava S, Nandakumar MP, Roy A, Wenger KS, Marten MR. Pulsed feeding during fed-batch fungal fermentation leads to reduced viscosity without detrimentally affecting protein expression. Biotechnol Bioeng 2003;81:341-7. https://doi.org/10.1002/bit.10481
  9. Reese ET, Maguire A. Surfactants as stimulants of enzyme production by microorganisms. Appl Microbiol 1969;17:242-5.
  10. Lucatero S, Galindo E, Larralde-Corona CP. Quantitative characterisation of the morphology of Trichoderma harzianum cultured in shake-flasks and containing Tween 40. Biotechnol Lett 2004;26:41-4. https://doi.org/10.1023/B:BILE.0000009458.58602.d9
  11. Ghose TK. Measurement of cellulase activities. Pure Appl Chem 1987;59:257-68. https://doi.org/10.1351/pac198759020257
  12. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54. https://doi.org/10.1016/0003-2697(76)90527-3
  13. Ohnishi ST, Barr JK. A simplified method of quantitating protein using the biuret and phenol reagents. Anal Biochem 1978;86:193-200. https://doi.org/10.1016/0003-2697(78)90334-2
  14. De Terra N, Tatum EL. A relationship between cell wall structure and colonial growth in Neurospora crassa. Am J Bot 1963;50:669-77. https://doi.org/10.2307/2440044
  15. Qing Q, Yang B, Wyman CE. Impact of surfactants on pretreatment of corn stover. Bioresour Technol 2010;101: 5941-51. https://doi.org/10.1016/j.biortech.2010.03.003