한국어류학회지 (Korean Journal of Ichthyology)

  • 제29권1호
  • /
  • Pages.1-12
  • /
  • 2017
  • /
  • 1225-8598(pISSN)
  • /
  • 2288-3371(eISSN)
한국어류학회 (The Ichthyological Society of Korea)
권호 표지

낙동강 상류 황지천에 서식하는 쉬리속(genus Coreoleuciscus) 어류 집단의 종 동정 및 잡종 판별

Species and Hybrid Identification of Genus Coreoleuciscus Species in Hwnag-ji Stream, Nakdong River Basin in Korea

  • 송하윤 (국립수산과학원 중앙내수면연구소) ;
  • 김재훈 (순천향대학교 생명시스템학과) ;
  • 서인영 (순천향대학교 생명시스템학과) ;
  • 방인철 (순천향대학교 생명시스템학과)
  • Song, Ha-Yoon (Inland Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Jae-Hun (Department of Life Science, Soonchunhyang University) ;
  • Seo, In-Young (Department of Life Science, Soonchunhyang University) ;
  • Bang, In-Chul (Department of Life Science, Soonchunhyang University)
  • 투고 : 2017.02.01
  • 심사 : 2017.03.15
  • 발행 : 2017.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

낙동강 상류 지류인 황지천에서 쉬리(Coreoleuciscus splendidus)와 참쉬리(C. aeruginosa)의 종 간 자연잡종 개체를 채집하였다. 쉬리와 참쉬리의 종 간 잡종 개체는 외부형태 비교와 함께 핵 DNA의 RAG1 유전자(1,334 bp)와 미토콘드리아 DNA인 CO1 유전자(1,551 bp)를 이용한 염기서열 분석을 실시하였다. 외부형태 분석결과 잡종 개체는 등지느러미, 꼬리지느러미 및 뒷지느러미 3곳에서 지느러미 반문의 형태가 쉬리와 참쉬리의 중간 형태를 나타내었다. RAG1과 CO1 유전자를 이용한 분자계통 분석결과 황치천에 분포하는 쉬리속 어류는 쉬리, 참쉬리 두 종과 두 종 간의 잡종 개체군으로 구성되어 있음을 확인하였으며, CO1 유전자의 염기서열 분석결과 순종인 정교배체와 잡종인 상반교배체가 잘 구분되었다. 또한 RAG1 유전자 분석결과 13개의 염기서열 변이를 확인하였고, 잡종 개체는 9개의 염기서열에서 double peaks가 확인되었다. 유전학적 분석과 외부형태 변이 분석에 의해 쉬리와 참쉬리 사이에 잡종화가 발생한 것을 확인하였으나 잡종 F2세대와 잡종 F1 세대의 생식적 격리 여부는 확인하지 못하였다.

A natural hybrid of interspecific between the Coreoleuciscus splendidus and C. aeruginos (Cypriniformes: Cyprinidae) was captured in the Hwang-ji Stream, a tributary of the Nakdong River basin in Korea. An interspecific hybrid between C. splendidus and C. aeruginos was genetically identified based on morphological characteristics and the sequence analysis of nuclear recombination activating gene 1 (RAG1) gene (1,334 bp) and mitochondrial cytochrome c oxidase subunit 1 (CO1) gene (1,551 bp). As a result of morphological variations, the natural hybrid appeared to have an intermediate character between two parental species (C. splendidus and C. aeruginos) in three variations of black array (s) on dorsal, caudal and anal fin rays. Phylogenetic analysis inferred from RAG1 and CO1 sequence data revealed that Coreoleuciscus populations from Hwang-ji stream consist of two pure Coreoleuciscus species and a hybrid individual group. The individuals were clearly identified the cross and reciprocal hybrid by CO1 gene analysis. In RAG1 gene, 13 nucleotide variation loci were detected and the hybrid individuals displayed the double peaks of sequence chromatograms at the 9 diagnostic positions. In this study, molecular data and morphological variations were clearly demonstrated that hybridization did occur between C. splendidus and C. aeruginos. However, F2 hybrid generation and reproductive capacity of F1 hybrid individuals were not demonstrated.

키워드

참고문헌

  1. April, J., R.L. Mayden, R.H. Hanner and L. Bernatchez. 2011. Genetic calibration of species diversity among North america's freshwater fishes. Nat. Acad. Sci., 108: 10602-10607. https://doi.org/10.1073/pnas.1016437108
  2. Asahida, T., T. Kobayashi, K. Saitoh and I. Nakayama. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fish. Sci., 62: 727-730.
  3. Byeon, H.K. and J.K. Oh. 2015. Fluctuation of fish community and inhabiting status of introduced fish in Gangeumgnamdae stream, Korea. Korean J. Environ Ecol., 29: 718-728. (in Korean) https://doi.org/10.13047/KJEE.2015.29.5.718
  4. Candolin, U. and J.D. Reynolds. 2002. Why do males tolerate sneakers? Tests with the European bitterling, Rhodeus sericeus. Behav. Ecol. Sociobiol., 51: 146-152. https://doi.org/10.1007/s00265-001-0422-6
  5. Cebrat, A., A. Cebula, A. Laszkiewicz, M. Kasztura, A. Miazek and P. Kisielow. 2008. Mechanism of lymphocyte-specific inactivation of RAG-2 intragenic promoter of NWC: Implications for epigenetic control of RAG locus. Mol. Immunol., 45: 2297-2306. https://doi.org/10.1016/j.molimm.2007.11.009
  6. Chae, B.S. 2003. Introduction of native fish species to the other river system. Case study. In: The introduced fishes of Korea. The Ichthyological Society, pp. 65-86. (in Korean)
  7. Chae, B.S., S.K. Kim, Y.H. Kang, N.S. Heo, J.M. Park, H.U. Ha and U.W. Hwang. 2015. Ichthyofauna and fish community structure in upper reach of the Nakdong River, Korea. Korean J. Ichthyol., 27: 116-132. (in Korean)
  8. Chevassus, B. 1983. Hybridization in fish. Aquaculture, 33: 245-262. https://doi.org/10.1016/0044-8486(83)90405-2
  9. Choi, C.G., Y.J. Hwang, M.S. and M.S. Na. 1995. A natural hybrid between spinous laoch, Cobitis longicorpus and cyprinid loach Misgurunus anguilicaudatus (Pices, Cobitis). Korean J. Ichthyol., 7: 203-207. (in Korean)
  10. Epifanio, J. and D. Philipp. 2001. Simulating the extinction of parental lineages from introgressive hybridization: the effects of fitness, initial proportions of parental taxa, and mate choice. Rev. Fish Biol. Fisheries, 10: 339-354.
  11. Hall, T.A. 1999. BioEdit: A user-friendly biogical sequence aligment editor and analysis program for windows 95/98/NT. Nucl. Acids. Symp. Set., 41: 95-98.
  12. Hauswith, W.W. and D.A. Clayton. 1985. Length heterogeneity of a conserved displacement-loop sequence in human mitochondrial DNA. Nucl. Acids Res., 13: 8093-8104. https://doi.org/10.1093/nar/13.22.8093
  13. Huelsenbeck, J.P. and F.R. Ronquise. 2001. MRBYES: bayesian inference of phylogeny. Bio-informatics, 17: 754-755.
  14. Jang, M.H., G.I. Cho and G.J. Joo. 2001. Fish fauna of the main channel in the Nakdong river. Korean J. Limnol., 34: 223-238. (in Korean)
  15. Jansson, H., U. Holmgrea, K. Wedin and T. Andersson. 1991. High frequency of natural hybrids between trout, S. trutta L., in a Swedish river. J. Fish Biol., 39: 343-348. https://doi.org/10.1111/j.1095-8649.1991.tb05096.x
  16. Kanoh, Y. 2000. Reproductive success associated with territoriality, sneaking, and grouping in male rose bitterings, Rhodeus ocellatus (Pisces: Cyprinidae). Environ. Biol. Fishes, 57:143-154. https://doi.org/10.1023/A:1004585405848
  17. Kim, C.H., K.E. Hong, J.H. Kim and K.H. Kim. 2006a. Ichthyofauna in Yeongok stream, ganneung, Korea. Korean J. Ichthyol., 18: 244-250. (in Korean)
  18. Kim, C.H., W.O. Lee, K.E. Hong, C.H. Lee and J.H. Kim. 2006b. Icthyofauna and fish community structure in Namdae stream, Yangyang, Korea. Koran J. Ichthyol., 18: 112-118. (in Korean)
  19. Kim, C.H., W.O. Lee, Y.J. Kang and J.M. Baek. 2010. Occurrence of a natural intergeneric hybrid, Rhodeus uyekiixAcheilognathus signifer (Pisces: Cyprinidae) from Jojongcheon Bukhan River, Korea. Korean J. Ichthyol., 22: 225-229. (in Korean)
  20. Kim, H.S., S.W. Yun, J.G. Ko and J.Y. Park. 2014. Occurrence of a natural intergeneric hybrid between Rhodeus pseudosericeus and Acheilognathus signifer (Pisces: Cyprinidae) from the Namhangang (river), Korea. Koran J. Ichthyol., 26: 153-158.
  21. Kim, I.S. 1997. Illustrated encycolopedia of fauna & flora of Korea, Vol. 37, Freshwater fishes. Ministry of Education, Yeongi, pp. 216-271. (in Korean)
  22. Kim, I.S. and W.O. Lee. 1984. Effects of stream modification in the Seomjin river on the fish communities of the Dongjin river in Korea. Bull. Korean Fish. Soc., 17: 549-556. (in Korean)
  23. Kim, I.S., Y. Choi, C.L. Lee, Y.J. Lee, B.J. Kim and J.H. Kim. 2005. Illustrated book of Korean fishes. Kyohak Publishing Co. Ltd., Seoul, 615pp. (in Korean)
  24. Kim, K.S., H.R. Lee, A. Sade and I.C. Bang. 2014. Identification of hybrid between the tiger grouper Epinephelus fuscoguttatus and the giant grouper E. lanceolatus by analyzing COX I and RAG 2 sequences. Korean J. Ichthyol., 26: 70-73. (in Korean)
  25. Kim, K.Y., M.H. Ko, S.J. Cho, W.J. Kin, M.H. Son and I.C. Bang. 2015. A natural hybrid of intergeneric mating between a female Puntungia herzi and a male Pseudorasbora parva (Cypriniformes: Cyprinidae). Fish. Aquat. Sci., 18: 99-107.
  26. Kim, M.J. and C.B. Song. 2011. Origin of the Korean mandarin fish, Siniperca scherzeri and molecular phylogenetic relationships to other Siniperca fishes. Koran J. Ichthyol., 23: 95-105. (in Korean)
  27. Ko, M.H. 2009. Reproductive mechanisms of the unisexual diploidtriploid hybrid complex of between the spine loach Cobitis hankugensis and Iksookimia longicorpa (Teleostei, Cobitis) in Korea. Doctors's thesis, Chonbuk National University, 160pp. (in Korean)
  28. Kuriiwa, K., N. Hanzawa, T. Yoshino, S. Kimura and M. Nishida. 2007. Phylogenetic relationships and natural hybridization in rabbitfishes (Teleostei: Siganidae) inferred from mitochondrial and nuclear DNA analysis. Mol. Phyl. Ecol., 45: 69-80. https://doi.org/10.1016/j.ympev.2007.04.018
  29. Kwan, Y.S., M.H. Ko and Y.J. Won. 2014. Genomic replacement of native Cobitis lutheri with introduced C. tetralineata through a hybrid swarm following the artificial connection of ricer systems. Ecol. Evol., 4: 1451-1465. https://doi.org/10.1002/ece3.1027
  30. Lee, I.R., H. Yang, J.H. Kim, K.Y. Kim and I.C. Bang. 2009. Identification of a natural hybrid between the striped spine loach Cobitis tetralineata and the king spine loach Iksookimia longicorpa by analyzing mitochondrial COI and nuclear RAG1 sequence. Koran J. Ichthyol., 21: 287-290. (in Korean)
  31. Lee, T.Y. and S.Y. Kim. 1987. Experimental studies on the mechanism of reproductive cycle in the bluegill, Lepomis macrochirus. Korean J. Fish. Aquat. Sci., 20: 489-500. (in Korean)
  32. Lee, W.O., I.R. Lee, H.Y. Song and I.C. Bang. 2008. Genetic differentiation of the largemouth bass Micropterus salmoides from the major rivers and reservoirs in Korea assessed by AFLP. Korean J. Limnol., 41: 395-401. (in Korean)
  33. Mori, T. 1935. Studies on the geographical distribution of freshwater fish in Korea. Bull. Biogeogr. Soc. Jap., 7: 35-61.
  34. NFRDI. 2007. Ecological survey and management strategy of introduced freshwater fishes. Report of national fisheries research and development institute (inland fisheries research institute). Gapyeong, Korea, 81pp. (in Korean)
  35. Park, S.C., K.Y. Lee, T.B. Choi, W.M. Kim, M.J. Kim and J.S. Choi. 2013. The fish fauna and disturbance of geographical distributions in the eastern civilian control line of Korea. J. Environ. Impact Assess., 22: 27-37. (in Korean) https://doi.org/10.14249/eia.2013.22.1.027
  36. Slechtova, V., J. Bohlen and H.H. Tan. 2007. Families of Cobitidea (Teleostei: Cypriniformes) as revealed from nuclear genetic data and the position of the mysterious genera Barbucca, Psilorhynchus, Swepenticobitis and vaillantella. Mol. Evol., 44: 1358-1365. https://doi.org/10.1016/j.ympev.2007.02.019
  37. Smith, C., M. Reichard, P. Jurajda and M. Przybylski. 2004. The reproductive ecology of the European bitterling (Rhodeus sericeus). J. Zool., Lond., 262: 107-124. https://doi.org/10.1017/S0952836903004497
  38. Song, H.B. and O.K. Kwon. 1993. Ecology of Coreoleuciscus splendidus Mori (Cyprinidae) in Hongchon River. Korean J. Limnol., 3: 235-244. (in Korean)
  39. Song, H.B. and G.M. Park. 2006. A molecular genetic variation among intra-populations of Korean shiner, Coreoleucuscus splendidus Mori (Cyprinidae)., Koran J. Ichthyol., 18: 78-86.
  40. Song, H.B., M.S. Byeon, D.W. Kang, C.Y. Jang, J.S. Moon and H.K. Park. 2012. Population structure of bluegill, Lepomis macrochirus in lakes of the Han river system, Korea. Koran J. Ichthyol., 24: 278-286. (in Korean)
  41. Song, H.H. 1976. Study on the spawning and early development of Coreoleuciscus splendidus Mori. Master's thesis, Kyunghee University, 23pp. (in Korean)
  42. Song, H.Y. and I.C. Bang. 2009. Genetic variation of Coreoleuciscus splendidus populations from four major river in Korea as assessed by RAPD PCR. Korean J. Ichrhyol., 21: 129-133. (in Korean)
  43. Song, H.Y. and I.C. Bang. 2015. Coreoleuciscus aeruginos (Teleostei: Cypriniformes: Cyprinidae), a new species from the Seomjin and Nakdong rivers, Korea. Zootaxa, 3931: 140-150. https://doi.org/10.11646/zootaxa.3931.1.10
  44. Song, H.Y., K.Y. Kim, M.G. Yoon, Y.K. Nam, D.S. Kim and I.C. Bang. 2010. Genetic variation of Coreoleuciscus splendidus population (Teleostei; Cypriniformes) from four major river drainage system in South Korea as assessed by AFLP markers. Genes & Genomics, 32: 199-205. https://doi.org/10.1007/s13258-009-0881-0
  45. Sonnenberg, R., A.W. Nolte and D. Tautz. 2007. An evaluation of LSU rDNA D1-D2 sequences for their use in species identification. Frontiers in Zool., 4: 6. https://doi.org/10.1186/1742-9994-4-6
  46. Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins. 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nuclecis Acids Res., 24: 173-216.
  47. Wendel, J.F. and J.J. Doyle. 1998. Phylogenetic incongruence: window into genome history and molecular evolution. In: Soltis, D.E., Soltis, P.S., Doyle, J.J. (eds.), Molecular systematics of plants II: DNA sequencing. Kluwer academic publishers, Boston, pp. 265-296.
  48. Wu, G., J. Luo, K. Chen, C. Xian, G. Lin, Z. Wang, Z. Lin, J. Luo and K. Pan. 1990. Analysis and comparison of morphology and cytology between the F sub (1) hybrid catfish (Claris fuscusxC. lazera) and its parents. Acta Hydrobiologica Sinica, Wnhan., 14: 328-335.
  49. Yoon, Y.E., I.R. Lee, S.Y. Park, E.J. Kang, E.O. Kim, S.K. Yang, Y.K. Nam and I.C. Bang. 2009. Genetic identification of hybrids between Rhodeus uyekii and R. notatus by sequence analysis of RAG-1 gene. J. Aquacult., 22: 79-82. (in Korean)