International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Expression of Recombinant Human Bone morphogenetic protein 2 (hBMP2) in Insect cells

  • Kim, Seong-Wan (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Park, Seung Won (Department of Biotechnology, Catholic University of Daegu) ;
  • Goo, Tae-Won (Department of Biochemistry, School of Medicine, Dongguk University) ;
  • Choi, Kwang-Ho (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
  • Received : 2017.03.28
  • Accepted : 2017.03.29
  • Published : 2017.03.31
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Abstract

Bone morphogenetic protein 2 (BMP2) plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is involved also in cardiac cell differentiation and epithelial to mesenchymal transition. In this study, We expressed human BMP2 (hBMP2) recombinant protein using Baculovirus Expression Vector System (BEVS) in Sf9 insect cells. The hBMP2 cDNA was cloned into baculovirus transfer vector, pBacgus-4x-1 and recombinant baculovirus was screened out through X-gal and GUS-fusions assay. Western blot analysis shown that molecular weight of hBMP2 recombinant protein was about 44.71 kDa.

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References

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