Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Characterization of auxin production plant growth promotion by a bacterium isolated from button mushroom compost

  • Yoo, Ji-Yeong (Department of Bio-Environmental Chemistry, College of Agriculture and Life sciences, Chungnam National University) ;
  • Lee, Heon-Hak (Department of Bio-Environmental Chemistry, College of Agriculture and Life sciences, Chungnam National University) ;
  • Han, Chang-Hoon (Department of Bio-Environmental Chemistry, College of Agriculture and Life sciences, Chungnam National University) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Life sciences, Chungnam National University)
  • Received : 2017.02.21
  • Accepted : 2017.03.23
  • Published : 2017.03.31
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Abstract

An auxin-producing bacterial strain, designated 4-3, was isolated from waste button mushroom compost in Boryeong-si, Chungnam. The strain 4-3 was classified as a novel strain of Leucobacter tardus, based on chemotaxonomic and phylogenetic analyses. TLC and HPLC the isolated L. tardus strain 4-3 produced indole-3-acetic acid (IAA), the auxin. Maximum IAA productionof $94.3mg\;L^{-1}$ was detected for bacteria cultured in R2A medium with 0.1% l-tryptophan, incubated for 24 h at $35^{\circ}C$. Negative correlationwas observed between IAA production and pH of the culture medium, indicating that the increase inIAA caused acidification ofthe medium. The effect of supplementation with varying concentrations of l-tryptophan, a known precursor of IAA, was also assessed. production was maximal at 0.1% l, but decreased at lconcentrations above 0.2%. To investigate the plant growth-promoting effects of the bacterium, L. tardus strain 4-3 culture broth was used to inoculate water cultures and seed pots of mung bean. We found thatadventitious root induction and root growth were 2.2-times higher in thethan in the non-inoculated plants.

Keywords

References

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