Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Current Status of Automatic Serial Sections for 3D Electron Microscopy

  • Choi, Hyosun (BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University) ;
  • Jung, Min Kyo (Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Mun, Ji Young (BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University)
  • Received : 2017.03.20
  • Accepted : 2017.03.30
  • Published : 2017.03.30
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Abstract

The automatic equipment for three-dimensional electron microscopy (3DEM) can acquire serial sections of a large sample in a relatively short time, and is especially suitable for the connectomics, which is a field related to understanding the brain structure as a whole. As many results obtained through 3DEM using automatic serial sections have been published in the field of brain research, many researchers continue to apply this technique to various samples. We reviewed the equipment for automatic serial sectioning, the block preparation method, the limitations of 3DEM, and future directions.

Keywords

References

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